Abstract
In this study, Sephadex G-50 and CM Sepharose Fast Flow were used to purify the spermatozoa of Takifugu obscurus. Identification of protamine by arginine color reaction. The effects of protamine on the minimum inhibitory concentration, inhibition zone diameter and growth curve of Staphylococcus aureus, Escherichia coli and Salmonella were determined. The results showed that: The spermatozoa of Takifugu obscurus had an absorption peak at 217 and 273 nm, respectively, and the absorption peak at 217 nm was higher. The purified protamine was found to be better than the protamine before purification by SDS-PAGE electrophoresis. The strips were fine and had no tailing. The Takifugu obscurus protamine had inhibitory effects on Gram-negative bacteria (Salmonella and Escherichia coli) and Gram-positive bacteria (Staphylococcus aureus), which could delay the bacteria growth, and the minimum inhibitory concentrations against Staphylococcus aureus, Escherichia coli and Salmonella were 500, 1 000 and 1 000 mg/L, respectively.
Publication Date
1-28-2020
First Page
171
Last Page
174,197
DOI
10.13652/j.issn.1003-5788.2020.01.030
Recommended Citation
Jia-yuan, ZHANG; Shun-sheng, CHEN; and Hong-cai, ZHANG
(2020)
"Study on purification and antibacterial activity of protamine from Takifugu obscurus,"
Food and Machinery: Vol. 36:
Iss.
1, Article 30.
DOI: 10.13652/j.issn.1003-5788.2020.01.030
Available at:
https://www.ifoodmm.cn/journal/vol36/iss1/30
References
[1] 朱永乐,李通,孟志云,等.鱼精蛋白模拟肽的体外代谢稳定性和代谢酶表型研究[J].国际药学研究杂志,2018,45(4):295-300.
[2] LUNGILE N T,SULAY T,FEDERICO B,et al.Protamine nanocapsules as carriers for oral peptide delivery[J].Journal of Controlled Release:Official Journal of the Controlled Release Society,2018,291:157-168.
[3] GRIFFIN M J,RINDER H M,SMITH B R,et al.The effects of heparin,protamine,and heparin/protamine reversal on platelet function under conditions of arterial shear stress[J].Anesthesia and Analgesia,2001,93(1):20-27.
[4] MC CLEAN M,DEBEVERE J.Evaluation of antimi-crobial activites of protamine[J].Food Miccrobiology,1994(11):417-427.
[5] HOSOMI R,MIYAUCHI K,YAMAMOTO D,et al.Salmon protamine decreases serum and liver lipid contents by inhibiting lipid absorption in an in vitro gastrointestinal digestion model and in rats[J].Journal of Food Science,2015,80(10):H2 346-H2 353.
[6] 律海伦,马家驹,胡晓飞,等.粉红马哈鱼鱼精蛋白的提取纯化及抑菌性初探[J].食品科技,2019,44(3):239-243.
[7] 曹文红,刘忠嘉,林芸,等.中巨石斑鱼精巢鱼精蛋白提取工艺优化及其抗菌活性的初步研究[J].食品科技,2017(11):267-271.
[8] 刘淑集,陈梧英,许旻,等.菊黄东方鲀鱼精蛋白提取工艺优化研究[J].渔业现代化,2019,46(2):75-82.
[9] 徐明生,陈锦屏,上官新展.鲤鱼抗菌精蛋白的分离纯化及鉴定[J].食品工业科技,2004,25(10):51-54.
[10] 胡晓璐,刘淑集,吴成业.鱿鱼鱼精蛋白的提取工艺优化研究[J].渔业研究,2013,35(6):434-440.
[11] 朱艳蕾.细菌生长曲线测定实验方法的研究[J].微生物学杂志,2016(5):108-112.
[12] TAO Yan,ZHAO Dong-mei,WEN Ya.Expression,purification and antibacterial activity of the channel catfish hepcidin mature peptide[J].Protein Expression and Purification,2014,94:73-78.
[13] 张佳,王莹,张峰,等.滤纸片法测定黄花蒿提取物对霉菌的抑制活性[J].湖北农业科学,2009,48(5):1 153-1 154.
[14] 胡晓璐.柔鱼鱼精蛋白的提取纯化及抑菌特性研究[D].福州:福建农林大学,2012:35-38.
[15] 董欣,王丽燕.氨基酸紫外光谱的再测定[J].德州学院学报,2015(2):44-46.
[16] 刘燕妮.鱼精蛋白的制备、纯化及其絮凝和抑菌活性研究[D].青岛:中国海洋大学,2015:46-52.
[17] 刘红玉,姜学芬,崔洪斌.大马哈鱼鱼精蛋白的提取及抑菌作用的研究[J].食品科学,2007,28(2):37-39.
[18] 张黎丽,马梦琳,李晨熙,等.蛋清溶菌酶的分离纯化及其抑菌活性研究[J].生物化工,2017,3(1):13-16.