Research progress in the detection technology of chloramphenicol residues in animal derived food

Authors

YUAN Zhe, Research Institute of Agricultural Quality Standards and Testing Technology, Hubei Academy of Agricultural Science, Wuhan, Hubei 430064, China;Hubei Key Laboratory of Nutritional Quality and Safety of Agro products, Wuhan, Hubei 430064, China;Yangtze University, Jingzhou, Hubei 434023, China
PENG Maomin, Research Institute of Agricultural Quality Standards and Testing Technology, Hubei Academy of Agricultural Science, Wuhan, Hubei 430064, China;Hubei Key Laboratory of Nutritional Quality and Safety of Agro products, Wuhan, Hubei 430064, China
LIU Li, Research Institute of Agricultural Quality Standards and Testing Technology, Hubei Academy of Agricultural Science, Wuhan, Hubei 430064, China;Hubei Key Laboratory of Nutritional Quality and Safety of Agro products, Wuhan, Hubei 430064, China
PENG Xitian, Research Institute of Agricultural Quality Standards and Testing Technology, Hubei Academy of Agricultural Science, Wuhan, Hubei 430064, China;Hubei Key Laboratory of Nutritional Quality and Safety of Agro products, Wuhan, Hubei 430064, China
XIA Hong, Research Institute of Agricultural Quality Standards and Testing Technology, Hubei Academy of Agricultural Science, Wuhan, Hubei 430064, China;Hubei Key Laboratory of Nutritional Quality and Safety of Agro products, Wuhan, Hubei 430064, ChinaFollow

Corresponding Author(s)

夏虹（1969—），女，湖北省农业科学院农业质量标准与检测技术研究所研究员，学士。E-mail：870189737@qq.com刘丽（1988—），女，湖北省农业科学院农业质量标准与检测技术研究所副研究员，博士。E-mail：liuli@hbaas.ac.cn

Abstract

Chloramphenicol is a widely used bactericide and highly effective antibiotic in aquaculture and livestock breeding. To protect human health, detecting chloramphenicol residues in animal-derived food products has become increasingly important. This review introduces the properties of chloramphenicol, the limit standards, and the hazards of residual chloramphenicol in animal-derived food products. Furthermore, it describes commonly used chloramphenicol detection methods. In addition, the review thoroughly analyzes and summarizes the advantages and disadvantages of different detection methods, outlining methods to improve the efficiency and precision of chloramphenicol detection to ensure the quality and safety of animal-derived food products. Finally, the prospects for developing advanced detection techniques and analytical methods are prospected.

Publication Date

1-30-2024

First Page

219

Last Page

225

DOI

10.13652/j.spjx.1003.5788.2023.80522

Recommended Citation

Zhe, YUAN; Maomin, PENG; Li, LIU; Xitian, PENG; and Hong, XIA (2024) "Research progress in the detection technology of chloramphenicol residues in animal derived food," Food and Machinery: Vol. 40: Iss. 1, Article 32.
DOI: 10.13652/j.spjx.1003.5788.2023.80522
Available at: https://www.ifoodmm.cn/journal/vol40/iss1/32

References

[1] 李昊. 中国水产品质量安全政策对比研究[J]. 中国渔业质量与标准, 2021, 11（4）: 54-61. LI H. Chinese aquatic products quality safety and policies comparative study[J]. Chinese Fishery Quality and Standards, 2021, 11（4）: 54-61.
[2] 罗昭军, 陈幸, 单乃荣, 等. 高效液相色谱法检测鸡蛋中氯霉素类药物残留[J]. 中国畜牧兽医文摘, 2018, 34（3）: 72-74. LUO Z J, CHEN X, SHAN N R, et al. Determination of chloramphenicol drugs in eggs by UPLC[J]. China Abstract Animal Husbandry and Veterinary Medicine, 2018, 34（3）: 72-74.
[3] 李广. 氟苯尼考及氟苯尼考胺药物残留检测方法及应用[J]. 辽宁农业科学, 2019（5）: 59-62. LI G. Method and application of florfenicol and florfenicol drug residue detection[J]. Liaoning Agricultural Sciences, 2019（5）: 59-62.
[4] 林敏霞, 徐媛原, 钟仕花. 优化高效液相色谱—串联质谱法检测水产品中酰胺醇类药物残留[J]. 现代食品, 2022, 28（1）: 209-212. LIN M X, XU Y Y, ZHONG S H. Optimization of high-performance liquid chromatography-tandem mass spectrometry for the detection of amide alcohol drug residues in aquatic products[J]. Modern Food, 2022, 28（1）: 209-212.
[5] 曹丽丽, 张书芬, 邢家溧, 等. 超高效液相色谱—串联质谱结合谱库检索快速测定鸡蛋中氯霉素、氟苯尼考和甲砜霉素残留[J]. 食品工业科技, 2020, 41（14）: 197-203. CAO L L, ZHANG S F, XING J L, et al. Fast determination of chloramphenicol, florfenicol, thiamphenicol in eggs by ultra performance liquid chromatography-tandem mass spectrometry combined with library search[J]. Science and Technology of Food Industry, 2020, 41（14）: 197-203.
[6] 王东鹏, 叶诚, 李小莎. Captiva EMR-Lipid固相萃取结合高效液相色谱—串联质谱法同时测定猪肉中氯霉素类药物残留量[J]. 食品安全质量检测学报, 2021, 12（19）: 7 660-7 666. WANG D P, YE C, LI X S. Simultaneous determination of chloramphenicol drugs residues in pork by Captiva EMR-Lipid solid phase extraction combined with high performance liquid chromatography-tandem mass spectrometry[J]. Journal of Food Safety and Quality, 2021, 12（19）: 7 660-7 666.
[7] 杨方, 方宇, 刘正才, 等. 液相色谱荧光检测法检测水产品中甲砜霉素、氟甲砜霉素及代谢物氟甲砜霉素胺残留[J]. 中国兽药杂志, 2008（8）: 13-16. YANG F, FANG Y, LIU Z C, et al. Determination of thiamphenicol, florfenicol and florfenicol amine residues in aquetic products by HPLC with fluorescence detection[J]. Chinese Journal of Veterinary Drug, 2008（8）: 13-16.
[8] 王亚群, 王静雪, 林洪, 等. 发光细菌法检测水产品中氯霉素体系的建立[J]. 中国海洋大学学报, 2009, 39（1）: 66-70. WANG Y Q, WANG J X, LIN H, et al. The establishment of chloramphenicol in aquatic products was detected by photobacterial method[J]. Journal of Ocean University of China, 2009, 39（1）: 66-70.
[9] 焦彦朝, 何家香, 梅先芝. 微生物检定法测定鲤鱼肌肉组织残留氯霉素[J]. 中国饲料, 2000（5）: 22-24. JIAO Y Z, HE J X, MEI X Z. The microbiological assay method was used to determine the residual chloramphenicol of carp muscle[J]. China Feed, 2000（5）: 22-24.
[10] 徐美奕, 孟庆勇. 养殖对虾中氯霉素残留的放射免疫分析[J]. 广州食品工业科技, 2003, 19（3）: 66-67. XU M Y, MENG Q Y. Application of RIA method for the determination of remained chloramphenicol in breed prawn[J]. Guangzhou Food Science and Technology, 2003, 19（3）: 66-67.
[11] ARNOLD D, SOMOGYI . Trace analysis of chloramphenicol residues in egg, milk and meat: Comparison of gas chromatography and radioimmunoassay[J]. Association of Official Analytical Chemists, 1985, 68（5）: 984-989.
[12] 刘智宏, 黄耀凌, 汪霞, 等. 水产品中甲砜霉素、氟苯尼考和氟苯尼考胺酶联免疫多残留测定[J]. 中国兽药杂志, 2010, 44（12）: 1-5. LIU Z H, HUANG Y L, WANG X, et al. Multi-residue determination of thiamphenicol, florfenicol and florfenicol in aquatic products by ELISA[J]. Chinese Journal of Veterinary Drug, 2010, 44（12）: 1-5.
[13] 区兑鹏, 卢义博, 严忠雍, 等. 酶联免疫法快速检测水产品中硝基呋喃类代谢物、氯霉素及氟苯尼考[J]. 中国渔业质量与标准, 2021, 11（1）: 27-33. OU D P, LU Y B, YAN Z Y, et al. Enzyme-linked immunoassay for the rapid detection of furan, chloramphenicol and florfenicol in aquatic products[J]. Chinese Fishery Quality and Standards, 2021, 11（1）: 27-33.
[14] 梁娟, 张倩玉, 魏海英, 等. 酶联免疫法测定水产苗种中氯霉素残留量[J]. 水产养殖, 2022, 43（3）: 49-52. LIANG J, ZHANG Q Y, WEI H Y, et al. Determination of chloramphenicol residues in aquatic seedlings by enzyme-linked immunoassay[J]. Journal of Aquaculture, 2022, 43（3）: 49-52.
[15] 许小炫, 苏晓娜, 谭庶, 等. 间接竞争化学发光酶联免疫分析方法检测禽肉中金刚烷胺和氯霉素残留[J]. 食品科学, 2021, 42（4）: 305-312. XU X X, SU X N, TAN S, et al. Determination of amantadine and chloramphenicol residues in poultry meat by indirect competitive chemiluminescence enzyme-linked immunosorbent assay[J]. Food Science, 2021, 42（4）: 305-312.
[16] 李倩, 陈煜, 阎安婷. 胶体金免疫层析法检测带鱼中氯霉素残留的前处理方法研究[J]. 现代食品, 2022, 28（20）: 182-184. LI Q, CHEN Y, YAN A T. Study on the pretreatment method for the detection of chloramphenicol residues in hairtail by colloidal gold immunochromatography[J]. Modern Food, 2022, 28（20）: 182-184.
[17] 杨秋红, 艾晓辉, 李荣, 等. 固相萃取—气相色谱法同时检测水产品中的氯霉素、甲砜霉素、氟苯尼考和氟苯尼考胺[J]. 分析试验室, 2015, 34（5）: 533-537. YANG Q H, AI X H, LI R, et al. Simultaneous determination of chloramphenicol, thiamphnicol, florfenicol and forfenicol-amine in aquatic products by gas chromatographic method with solid phase extraction[J]. Chinese Journal of Analysis Laboratory, 2015, 34（5）: 533-537.
[18] 庄姜云, 刘建芳, 李红权, 等. 内标液相色谱—串联质谱法测定蜂蜜中氯霉素、甲砜霉素和氟苯尼考残留[J]. 食品安全质量检测学报, 2021, 12（5）: 1 966-1 971. ZHUANG J Y, LIU J F, LI H Q, et al. Determination of chloramphenicol,sulfoxycin and fluorphenicol residues in honey by liquid chromatography-tandem mass spectrometry with interior label[J]. Journal of Food Safety and Quality, 2021, 12（5）: 1 966-1 971.
[19] 刘洋, 邢海艳, 苏晶, 等. 液相色谱—串联质谱法测定鸡蛋中氯霉素和氟苯尼考残留量的研究[J]. 中国食品添加剂, 2021, 32（5）: 101-106. LIU Y, XING H Y, SU J, et al. Determination of chloramphenicol and florfenicol residues in eggs by liquid chromatography-tandem mass spectrometry[J]. China Food Additives, 2021, 32（5）: 101-106.
[20] 王安伟, 覃锐, 刘天密, 等. 高效液相色谱法检测罗非鱼中氯霉素的残留量[J]. 食品安全质量检测学报, 2018, 9（7）: 1 665-1 668. WANG A W, QIN R, LIU T M, et al. Determination of chloramphenicol residues in tilapia by high performance liquid chromatography[J]. Journal of Food Safety and Quality, 2018, 9（7）: 1 665-1 668.
[21] 章琦, 赵薇, 韩迎红. 氯霉素磁性分子印迹聚合物的合成及应用[J]. 分析科学学报, 2020, 36（2）: 212-216. ZHANG Q, ZHAO W, HAN Y H. Preparation and application of chloramphenicol magnetic molecular imprinted polymers[J]. Journal of Analytical Science, 2020, 36（2）: 212-216.
[22] 王安伟, 刘天密, 覃锐, 等. 水产品中氯霉素残留检测方法研究进展[J]. 食品安全质量检测学报, 2017, 8（11）: 4 259-4 264. WANG A W, LIU T M, QIN R, et al. Research progress on the detection of chloramphenicols residues in aquatic products[J]. Journal of Food Safety and Quality, 2017, 8（11）: 4 259-4 264.
[23] 刘进玺, 王铁良, 胡京枝, 等. QuEChERS结合HPLC-MS/MS同时测定鱼肉中多种兽药残留[J]. 食品与发酵工业, 2021, 47（19）: 252-257. LIU J X, WANG T L, HU J Z, et al. Determination of veterinary drug residues in fish by QuEChERS method with HPLC-MS/MS method with HPLC-MS/MS[J]. Food and Fermentation Industries, 2021, 47（19）: 252-257.
[24] 邵会, 冷凯良, 周明莹, 等. 水产品中氯霉素、甲砜霉素、氟苯尼考、氟苯尼考胺多残留的同时测定-GC/MS法[J]. 渔业科学进展, 2015, 36（3）: 137-141. SHAO H, LENG K L, ZHOU M Y, et al. Simultaneous determination of residuchloramphenicol, thiamphenicol, florfenicol and florfenicol amine es in aquatic products with gas chromatography/mass spectrometry[J]. Progress in Fishery Sciences, 2015, 36（3）: 137-141.
[25] 劳哲, 江恩源, 朱国强. 在线凝胶渗透色谱—气相色谱串联质谱法测定动物源性食品中氯霉素[J]. 分析试验室, 2020, 39（6）: 726-730. LAO Z, JIANG E Y, ZHU G Q. Determination of chloramphenicol in animal derived food by on-line gel permeation chromatography-gas chromatography-tandem mass spectrometry[J]. Chinese Journal of Analysis Laboratory, 2020, 39（6）: 726-730.
[26] 张伟玮, 曾明华, 许世富, 等. 高效液相色谱—串联质谱法同时测定鸡肉中氯霉素类药物及其代谢物残留方法的建立[J]. 安徽农业大学学报, 2012, 39（4）: 641-645. ZAHNG W W, ZENG M H, XU S F, et al. Development of method for simultaneous determination of chloramphenicol, thiamphenicol, florfenicol and florfenicol amine residues in chicken tissues by LC-MS/MS[J]. Journal of Anhui Agricultural University, 2012, 39（4）: 641-645.
[27] 崔悦, 刘墨一, 曹冬, 等. 液相色谱—串联质谱法检测蜂蜜中氟苯尼考及其代谢物残留量[J]. 食品安全质量检测学报, 2021, 12（7）: 2 695-2 700. CUI Y, LIU M Y, CAO D, et al. Determination of florfenicol and its metabolite residues in honey by liquid chromatography-tandem mass spectrometry[J]. Journal of Food Safety and Quality, 2021, 12（7）: 2 695-2 700.
[28] 李龙. UPLC-MS/MS法快速测定水产品中氯霉素、甲砜霉素、氟苯尼考残留量[J]. 食品安全导刊, 2022（34）: 67-70. LI L. Rapid determination of chloramphenicol, thiamphenicol, and florfenicol residues in aquatic products by UPLC-MS/ MS method[J]. China Food Safety Magazine, 2022（34）: 67-70.
[29] 张贵会, 金定, 高友汉, 等. 超高压液相色谱—串联四极杆质谱法检测鸡蛋中氯霉素的残留量[J]. 现代食品, 2023, 29（5）: 208-211. ZHANG G H, JIN D, GAO Y H, et al. Determination of chloramphenicol residues in eggs by ultra-high pressure liquid chromatography tandem quadrupole mass spectrometry[J]. Modern Food, 2023, 29（5）: 208-211.
[30] 郑陆红, 张慧琼, 陈茹, 等. 超高效液相色谱—串联质谱法同时测定动物组织中氯霉素、甲砜霉素、氟苯尼考和其代谢产物氟苯尼考胺残留量[J]. 现代食品, 2022, 28（20）: 198-203. ZHENG L H, ZHANG H Q, CHEN R, et al. Determination of chloramphenicol, thiamphenicol, florfenicol and its metabolite florfenicol amine residues in animal tissues by UHPLC-MS/MS[J]. Modern Food, 2022, 28（20）: 198-203.
[31] 常波. QuEChERS-UPLC-MS/MS法快速测定水产品中氯霉素[J]. 食品安全导刊, 2023（3）: 92-94. CHANG B. Using QuEChERS-UPLC-MS/MS for rapid determination of chloramphenicol in aquatic products[J]. China Food Safety, 2023（3）: 92-94.
[32] 辛晓晨, 卫瑾瑾, 鹿尘, 等. 通过式固相萃取/超高效液相色谱—串联质谱法测定鸡蛋中氯霉素、氟苯尼考和氟苯尼考胺残留[J]. 中国食品卫生杂志, 2023, 35（9）: 1 297-1 303. XIN X C, WEI J J, LU C, et al. Determination of chloramphenicol, florfenicol and florfenicol amine residues in hen eggs by ultra-performance liquid chromatography-tandem mass spectrometry with pass-through solid extraction[J]. Chinese Journal of Food Hygiene, 2023, 35（9）: 1 297-1 303.
[33] 程慧, 刘顺, 黎波, 等. 基于DNA酶催化反应建立适配体比色法检测鸡蛋中氯霉素[J]. 食品与机械, 2021, 37（11）: 61-66. CHEN H, LIU S, LI B, et al. An aptamer colorimetric method was established to detect chloramphenicol in eggs based on DNase-catalyzed reaction[J]. Food & Machinery, 2021, 37（11）: 61-66.
[34] 戴小辉. 基于镍—金属有机框纳米酶活性的氯霉素核酸适配体传感器构建和应用[J]. 食品安全质量检测学报, 2022, 13（13）: 4 265-4 272. DAI X H. Construction and application of chloramphenicol aptasensor based on the nanozyme activity of nickel-metal-organic framework[J]. Journal of Food Safety and Quality, 2022, 13（13）: 4 265-4 272.
[35] 熊威威, 汪鹏, 李文恒, 等. 基于染料标记的核酸适配体对氯霉素的比率定量检测[J]. 武汉大学学报（理学版）, 2022, 68（4）: 405-412. XIONG W W, WANG P, LI W H, et al. Ratiometric quantitative detection of chloramphenicol based on dye-labeled aptamer[J]. Journal of Wuhan University （Natural Science Edition）, 2022, 68（4）: 405-412.
[36] 王玲, 管笛, 周欣, 等. 磁性化学发光酶免疫法检测猪肉中的氯霉素[J]. 食品科学, 2017, 38（10）: 305-309. WANG L, GUAN D, ZHOU X, et al. Development of magnetic bead-based chemiluminescent enzyme immunoassay for chloramphenicol detection in swine muscle[J]. Food Science, 2017, 38（10）: 305-309.
[37] 艾晨昊, 吴叶宇, 谭学才, 等. 基于CdS量子点的电致化学发光—分子印迹传感器检测氯霉素[J]. 分析试验室, 2019, 38（5）: 513-518. AI C H, WU Y Y, TAN X C, et al. A molecularly imprinted polymer electrochemiluminescence sensor based on CdS quantum dots for detection of chloramphenicol[J]. Chinese Journal of Analysis Laboratory, 2019, 38（5）: 513-518.
[38] 王鑫, 刘河冰, 陶晓奇. 基于核酸适配体检测动物性食品中氯霉素残留的研究进展[J]. 食品与发酵工业, 2019, 45（18）: 254-262. WANG X, LIU H B, TAO X Q. Research progress on detection of chloramphenicol residues in animal food based on nucleic acid aptamer[J]. Food and Fermentation Industries, 2019, 45（18）: 254-262.
[39] 詹峰萍, 吴杨仪, 戴小辉, 等. 基于Fe（III）-2-氨基对苯二甲酸金属有机框架的免标记氯霉素电化学传感器的构建及应用[J]. 分析化学, 2023, 51（4）: 539-548. ZHAN F P, WU Y Y, DAI X H, et al. Construction and application of Fe（III）-2-aminoterephthalic acid metal-organic framework-based label-free chloramphenicol electrochemical sensor[J]. Chinese Journal of Analytical Chemistry, 2023, 51（4）: 539-548.
[40] 崔乃元, 赵义良, 马立才, 等. 水产品中氯霉素时间分辨荧光免疫层析定量检测方法[J]. 食品与发酵工业, 2019, 45（24）: 241-245. CUI N Y, ZHAO Y L, MA L C, et al. Quantitative determination of chloramphenicol in aquatic products by time-resolved fluorescence immunochromatography[J]. Food and Fermentation Industries, 2019, 45（24）: 241-245.