Abstract
[Objective] This study aimed to improve the water solubility of propolis, and expand the application of propolis in food industry. [Methods] By utilizing the film dispersion method, propolis liposome was prepared with cholesterol and soy lecithin as the wall material and propolis as the core component. The optimal preparation process for propolis liposome was optimized through a combination of one-factor experiments and response surface methodology with the encapsulation rate as the test index, and its antioxidant activity was also analyzed and evaluated. [Results] The optimal process for preparing propolis liposome consisted of 2.5 g/100 mL soy lecithin, a cholesterol to soy lecithin ratio of 1∶10.3, and a cholesterol to propolis ratio of 1∶3.8. These optimized conditions resulted in a high encapsulation rate of 93.09% for propolis liposome. In addition, it was found that the scavenging efficiencies of the 50 μL/mL propolis liposome were 88.60%, 60.53%, and 82.25% for clearance of DPPH radicals, ABTS+ radicals, and hydroxyl radicals, respectively. [Conclusion] The prepared propolis liposome has high encapsulation efficiency, good water solubility and good antioxidant capacity.
Publication Date
7-22-2024
First Page
192
Last Page
198,232
DOI
10.13652/j.spjx.1003.5788.2023.81037
Recommended Citation
Siyuan, SUN; Lingjie, KONG; Hequan, ZHU; and Jie, DONG
(2024)
"Optimization of the preparation process for propolis liposome and antioxidant activity analysis,"
Food and Machinery: Vol. 40:
Iss.
6, Article 27.
DOI: 10.13652/j.spjx.1003.5788.2023.81037
Available at:
https://www.ifoodmm.cn/journal/vol40/iss6/27
References
[1] RIVERA-YAEZ C R, RUIZ-HURTADO P A, MENDOZA-RAMOS M I, et al. Flavonoids present in propolis in the battle against photoaging and psoriasis[J]. Antioxidants （Basel）, 2021, 10（12）: 2 014.
[2] BOUCHELAGHEM S. Propolis characterization and antimicrobial activities against Staphylococcus aureus and Candida albicans: a review[J]. Saudi Journal of Biological Sciences, 2022, 29（4）: 1 936-1 946.
[3] RUIZ-BUSTOS P, ALDAY E, GARIBAY-ESCOBAR A, et al. Propolis: antineoplastic activity, constituents, and mechanisms of action[J]. Current Topics in Medicinal Chemistry, 2023, 23（18）: 1 953-1 764.
[4] AL-HARIRI M. Immune's-boosting agent: immunomodulation potentials of propolis[J]. J Family Community Med, 2019, 26（1）: 57-60.
[5] 代旭栋, 李云, 李双双, 等. 皮肤外用脂质体的研究进展[J]. 国际药学研究杂志, 2020, 47（11）: 914-921.
DAI X D, LI Y, LI S S, et al. Liposomes for external use on skin: research advances[J]. Journal of International Pharmaceutical Research, 2020, 47（11）: 914-921.
[6] 颜红, 宫思璡, 于亮, 等. 脂质体作为药物载体的最新研究进展[J]. 药物生物技术, 2023, 30（3）: 325-330.
YAN H, GONG S J, YU L, et al. Recent advances in the study of liposomes as drug carriers[J]. Chinese Journal of Pharmaceutical Biotechnology, 2023, 30（3）: 325-330.
[7] 葛怡青, 汪浅, 仝涛. 蜂胶功能成分及生物活性研究进展[J]. 食品安全质量检测学报, 2022, 13（4）: 1 027-1 035.
GE Y Q, WANG Q, TONG T. Research progress on functional components and bioactivities of propolis[J]. Journal of Food Safety and Quality, 2022, 13（4）: 1 027-1 035.
[8] 袁菊. 蜂胶黄酮脂质体的制备及免疫增强作用的研究[D]. 南京: 南京农业大学, 2012: 17-28.
YUAN J. Studies on immunogical adjuvanticity of propolis flavonoids liposome[D]. Nanjing: Nanjing Agricultural University, 2012: 17-28.
[9] 陶阳. 蜂胶黄酮脂质体免疫增强活性的研究[D]. 南京: 南京农业大学, 2015: 15-24.
TAO Y. The studies on immunogical adjuvanticity of propolis flavonoids liposome[D]. Nanjing: Nanjing Agricultural University, 2015: 15-24.
[10] 张冰慧. 蜂胶脂质体的制备及其对高血糖小鼠降糖作用的研究[D]. 合肥: 合肥工业大学, 2009: 9-19.
ZHANG B H. Study on preparation of propolis liposome and its hypoglycemic effect[D]. Hefei: Hefei University of Technology, 2009: 9-19.
[11] 郭夏丽, 蓝雅惠, 邹艺红, 等. 蜂胶醇提物微胶囊的制备及表征[J]. 现代食品科技, 2016, 32（10）: 226-232.
GUO X L, LAN Y H, ZOU Y H, et al. Preparation and characterization of eep calcium alginate-chitosan microcapsules[J]. Modern Food Science and Technology, 2016, 32（10）: 226-232.
[12] 刘璐, 孙敬蒙, 景啸澜, 等. 改良—薄膜分散法优化栀子提取物类脂质体的制备工艺[J]. 华西药学杂志, 2019, 34（2）: 131-135.
LIU L, SUN J M, JING X L, et al. Optimization of the gardenia extract liposome based on Modified - film dispersion method[J]. West China Journal of Pharmaceutical Sciences, 2019, 34（2）: 131-135.
[13] 谢佳秀, 巫小媚, 陈红丽, 等. 三七总皂苷脂质体的制备及表征研究[J]. 时珍国医国药, 2021, 32（3）: 610-613.
XIE J X, WU X M, CHEN H L, et al. Preparation and characterization of liposomes of Panax ginseng total saponin[J]. Lishizhen Medicine and Materia Medica Research, 2021, 32（3）: 610-613.
[14] 罗照明. 中国蜂胶中多酚类化合物的研究[D]. 北京: 中国农业科学院, 2013: 11.
LUO Z M. Polyphenolic composition of Chinese propolis[D]. Beijing: Chinese Academy of Agricultural Sciences, 2013: 11.
[15] ILHAMI G, ERCAN B M, HILAL S, et al. Polyphenol contents and antioxidant activity of lyophilized aqueous extract of propolis from Erzurum, Turkey[J]. Food and Chemical Toxicology, 2010, 48（8/9）: 2 227-2 238.
[16] 李进, 刘世军, 罗锐, 等. 大枣叶水提醇沉物的抗氧化活性研究[J]. 陕西农业科学, 2023, 69（7）: 1-7, 17.
LI J, LIU S J, LUO R, et al. Antioxidant activity of water-extraction alcohol-precipitation extracts from jujube leaves[J]. Shaanxi Journal of Agricultural Sciences, 2023, 69（7）: 1-7, 17.
[17] 陈杰. 不同产地灵芝子实体化学成分及抗氧化活性研究[D]. 天津: 天津科技大学, 2016: 35-36.
CHEN J. The components and antioxidant activity of ganoderma lucidum from different origins[D]. Tianjin: Tianjin University of Science and Technology, 2016: 35-36.
[18] 孙丽萍, 徐响, 黄少康, 等. 蜂胶不同溶剂萃取物组成和抗氧化性的研究[J]. 食品科学, 2009, 30（21）:75-78.
SUN L P, XU X, HUANG S K, et al. Chemical compositions and antioxidant activity of different solvent extracts from propolis[J]. Food Science, 2009, 30（21）: 75-78.
[19] 谭曜, 方爱琴, 谢建敏, 等. 蜂胶乙醇溶液体外清除羟基自由基能力的测定[J]. 广东化工, 2021, 48（12）: 189-190, 182.
TAN Y, FANG A Q, XIE J M, et al. Study on effect of propolis ethanol solution on scavenging hydroxyl radical in vitro[J]. Guangdong Chemical Industry, 2021, 48（12）: 189-190, 182.