ERIC-PCR and Sau-PCR analysis of food-borne Listeria monocytogenes

Authors

ZHAO Yiming, College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, Guangdong 510640, China; Xiamen Yinxiang Group CO., Ltd, Xiamen, Fujiang 361100, China
SHI Lei, College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, Guangdong 510640, China; Xiamen Yinxiang Group CO., Ltd, Xiamen, Fujiang 361100, China
MENG Hecheng, College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, Guangdong 510640, China; Xiamen Yinxiang Group CO., Ltd, Xiamen, Fujiang 361100, China
ZHANG Zhigang, Xiamen Yinxiang Group CO., Ltd, Xiamen, Fujiang 361100, China
YAN He, College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, Guangdong 510640, China; Xiamen Yinxiang Group CO., Ltd, Xiamen, Fujiang 361100, China

Abstract

To investigate the distribution of serotypes, genetic relativity and epidemiological characteristic of Listeria monocytogenes (LM). 22 LM strains isolated from Guangzhou and Xiamen were investigated by ERIC-PCR and Sau-PCR. Results of ERIC-PCR showed that these strains were distributed in 8 patterns (a-h). The main pattern was pattern h, and the number of strains was 8. Genotyping using Sau-PCR showed that all tested strains were grouped into 7 clusters (A-G). These results indicated the genetic relativity and epidemiological characteristic of different LM food-borne isolates.

Publication Date

2-28-2015

First Page

54

Last Page

58

DOI

10.13652/j.issn.1003-5788.2015.01.012

Recommended Citation

Yiming, ZHAO; Lei, SHI; Hecheng, MENG; Zhigang, ZHANG; and He, YAN (2015) "ERIC-PCR and Sau-PCR analysis of food-borne Listeria monocytogenes," Food and Machinery: Vol. 31: Iss. 1, Article 12.
DOI: 10.13652/j.issn.1003-5788.2015.01.012
Available at: https://www.ifoodmm.cn/journal/vol31/iss1/12

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