Abstract
Two water-soluble polysaccharide fractions named as GLPD2 and GLPD2were isolated and purified from the same Ganoderma Lucidum body which was collected from Jiangxi province. The GPC and SEC-LLS results indicated that GLPD1 and GLPD2 were high purity, moreover, their molecule weights could both reach above 5×106. Their primary structural features and conformational properties were characterized by infrared spectrometry and SEC-LLS. The data obtained indicated that GLPD1 and GLPD2 may both be β-glucan configuration with pyran glucan groups. What's more, conformational analysis detected by SEC-LLS showed that GLPD1 and GLPD2 had rather tight structure with high degree of branch. However, PMP-HPLC analysis found out that GLPD1 and GLPD1 were quite different from each other. To clarify this, the Monosaccharide composition mole pencentage ratios were listed. GLPD1∶Man∶GlcN∶Rib∶Rham∶GalUA∶GalN∶Glc∶Gal∶Xyl∶Fuc=3.7∶0.8∶0.16∶6.8∶0.7∶0.29∶77∶9.27∶0.16∶1.1; GLPD2∶Man∶GlcN∶Rib∶Rham∶GalUA∶GalN∶Glc∶Gal=10.6∶2.0∶0.97∶10.72∶0.65∶0.99∶64.51∶9.52. In the end, compared the immunological activity with normal mice spleen lymphocyte proliferation promotion activity which were significant difference. It provide reliable basis for the culture of this kind of Ganoderma Lucidum.
Publication Date
2-28-2015
First Page
132
Last Page
136
DOI
10.13652/j.issn.1003-5788.2015.01.031
Recommended Citation
Hui, YANG; Jun, DAI; Shangwei, CHEN; and Song, ZHU
(2015)
"Study on isolation, purification, composition and immulogical characterics of Ganoderma Lucidum body polysaccharides,"
Food and Machinery: Vol. 31:
Iss.
1, Article 31.
DOI: 10.13652/j.issn.1003-5788.2015.01.031
Available at:
https://www.ifoodmm.cn/journal/vol31/iss1/31
References
[1] 谭伟，郑林用，郭勇，等. 灵芝生物学及生产新技术［M］. 北京：中国农业科学技术出版社，2007：1.
[2] 张晓云,杨春清.灵芝的化学成分和药理作用［J］. 国外医药·植物药分册，2006,21(4):152~155.
[3] 杨占涛，刘超，李月，等. 灵芝多糖的研究现状［J］. 安徽农业科学，2008，36（20）：8 651~8 652，8 739.
[4] 向俊宇，许安，夏悉，等. 灵芝多糖的研究进展［J］.药学实践杂志，2010，28(4)：241~244.
[5] 鉏晓艳，张日俊，张功，等. 灵芝多糖的结构及功能研究［J］.饲料研究, 2004（12）：37~39.
[6] Bao Xing-feng, Liu Cui-ping, Fang Ji-nian, et al. Structural and immunological studies of a major polysaccharide from spores of Ganoderma Lucidum（Fr.）Karst［J］. Carbohydrate Research, 2001（332）:67~74.
[7] Chen Yi, Xie Ming-Yong, Nie Shao-ping, et al. Purification, composition analysis and antioxidant activity of a polysaccharide from the fruiting bodies of Ganoderma atrum ［J］. Food Chemistry, 2008（107）: 231~241.
[8] Nie Shao-ping, Zhang Hui, Li Wen-juan, et al. Current development of polysaccharides from Ganoderma: Isolation, structure and bioactivities［J］.Bioactive Carbohydrates and Dietary Fibre,2013, 1(1):10~20.
[9] Huang Sheng-quan, Li Jin-wei, Li Yu-qiang,et al. Purification and structural characterization of a new water-soluble neutral polysaccharide GLP-F1-1 from Ganoderma lucidum［J］. International Journal of Biological Macromolecular, 2011(48):165~169.
[10] Dong Qun, Wang Ying, Shi Lei, et al. A novel water-solubleβ-D-glucan isolated from the spores of Ganoderma lucidum［J］. Carbonhydrate Research, 2012(353):100~105.
[11] Zhao Li-yan, Dong Yan-hong, Chen Gui-ting,et al. Extraction, purification, characterization and antitumor activity of polysaccharides from Ganoderma lucidum［J］. Carbonhydrate Polymers, 2010(80): 783~789.
[12] 曹鹏伟，彭奇均，戴军，等. D392树脂对灵芝多糖色素吸附行为研究［J］.化工技术与开发， 2009，38（7）：14~17.
[13] 张惟杰.糖复合物生化研究技术［M］. 杭州：浙江大学出版社，1994：32，46，89，256.
[14] Dai Jun, Wu Yan, Chen Shang-wei, et al. Sugar compositional determination of polysaccharides from Duneliella salina by modified RP—HPLC method of precolumn derivatization with 1-phenyl-3-methyl-5-pyrazolone ［J］. Carbohydrate Polymers, 2010, 82(3):629～635.
[15] 王浩豪 灵芝孢子粉多糖色谱指纹图谱及其免疫活性谱效关系的研究［D］. 无锡：江南大学,2012.
[16] 王建国. 灵芝多糖的结构及生物活性研究［D］. 武汉：武汉大学，2010.
[17] 李盛. 香菇三螺旋葡聚糖氢键键合作用及其功能化研究［D］. 武汉：武汉大学，2013.
[18] 张俐娜，薛奇，莫志深，等. 高分子物理近代研究方法［M］. 武汉：武汉大学出版社，2003.