Detection on phthalates in white spirit by HPLC

Authors

CAI Yingxiang, Faculty of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan 410004, China
LI Zhonghai, Faculty of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan 410004, China
FU Xiangjin, Faculty of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan 410004, China
ZHANG Hui, Faculty of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan 410004, China
LI Jilie, Faculty of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan 410004, China
WANG Shuyuan, Faculty of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan 410004, China

Abstract

A comprehensive analytical method based on high performance liquid chromatography (HPLC) was developed for measurement in foods of 6 phthalates-Dibutyl phthalate, Dioctyl Phthalate, Di-N-pentyl phthalate, Diethyl phthalate, Butyl benzyl phthalate, Diallyl phthalate. The extracts were analyzed by HPLC using an Inertsil C₁₈ column. The two kind of organic mobile phases which are acetonitril and the methanol were compared. The acetonitril-water gradient elusion was found to be more advantageous. Under the optimal conditions, the calibration curves showed a good linearity (R2＞0.999 0) in the concentration ranged from 1.0～20.0 mg/L for 4 target compounds. Recoveries of 6 analyses at three spiked levels ranged from 85.57% to 109.90%, with relative standard deviations (RSD) less than 10%. The detection limits of the method ranged from 0.179 to 0.425 mg/L. This method is sensitive and simple, and is suitable for the rapid determination of the phthalates added in the food.

Publication Date

4-28-2015

First Page

110

Last Page

113

DOI

10.13652/j.issn.1003-5788.2015.02.023

Recommended Citation

Yingxiang, CAI; Zhonghai, LI; Xiangjin, FU; Hui, ZHANG; Jilie, LI; and Shuyuan, WANG (2015) "Detection on phthalates in white spirit by HPLC," Food and Machinery: Vol. 31: Iss. 2, Article 23.
DOI: 10.13652/j.issn.1003-5788.2015.02.023
Available at: https://www.ifoodmm.cn/journal/vol31/iss2/23

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