Indirect determination of trace iron (Ⅲ) based on fluorescence quenching of Fe (Ⅲ)—KI—RB system

Authors

DONG Wenli, ChongQing University of Science and Technology, Chongqing, 400050, China
XU Gang, ChongQing University of Science and Technology, Chongqing, 400050, China

Abstract

This research is focused on the fluorescence properties and reaction conditions of Fe (Ⅲ)-KI-RB system. I₃^– is generated with Fe^{3 +} reaction with excessive KI in hydrochloric acid solution, which can react with rhodamine B to form Ion complexes to lead to quenching of inherent fluorescence of rhodamine B.The results shows that excitation wavelength（λex）is 352 nm and emission wavelength（λem）is 580 nm.The fluorescence intensity decreased linearly with the iron(Ⅲ) concentration in the range of 0.02～4.50 μg/25 mL. The detection limit is 0.006 μg/25 mL. It is applied to the determination of trace iron(Ⅲ) in peanuts with satisfactory results,which the relative standard deviation is less than 2%, and the average recovery rate of iron is between 96.7%～101% when adding standard solution to the samples.

Publication Date

4-28-2015

First Page

122

Last Page

124,133

DOI

10.13652/j.issn.1003-5788.2015.02.026

Recommended Citation

Wenli, DONG and Gang, XU (2015) "Indirect determination of trace iron (Ⅲ) based on fluorescence quenching of Fe (Ⅲ)—KI—RB system," Food and Machinery: Vol. 31: Iss. 2, Article 26.
DOI: 10.13652/j.issn.1003-5788.2015.02.026
Available at: https://www.ifoodmm.cn/journal/vol31/iss2/26

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