Abstract
The experiment is to do some research about features of nuts protein across extraction of some nuts. Using physical method such as high temperature and high pressure and microwave ultrasound combined with protein enzymatic processing nuts, explore the best method of removing nuts allergenic protein. Through experiment study found, cashew, almond, walnut protein lead to allergic reaction in body, but after high temperature and high pressure, ultrasonic - joint of microwave extraction and after protease treatment can reduce the allergenic obviously. The ultr- microwave and enzymatic 90 ℃, 240 s, microwave power 700 W, 0.25% trypsin with 5 minutes can almost completely removing nuts allergen protein, is the best way to desensitization.
Publication Date
4-28-2015
First Page
140
Last Page
143
DOI
10.13652/j.issn.1003-5788.2015.02.030
Recommended Citation
Jing, ZHAO; Ailin, ZHANG; Xinyi, HE; and Jiantao, FAN
(2015)
"Study on removal allergen technology of physical method combining enzymatict of on nuts,"
Food and Machinery: Vol. 31:
Iss.
2, Article 30.
DOI: 10.13652/j.issn.1003-5788.2015.02.030
Available at:
https://www.ifoodmm.cn/journal/vol31/iss2/30
References
[1] Merima Bublin,Heimo Breiteneder. Cross-reactivity of peanut allergens[J]. Current Allergy and Asthma Reports,2014, 14(1):426~453.
[2] 张爱琳,王昌禄,胡云峰,等. 坚果类过敏原的分离及免疫印迹分析[J]. 现代食品科技,2014,30(3):99~102.
[3] 李生平. 银杏种子萌发过程中贮藏物质代谢机理的研究[D]. 南京:南京林业大学, 2004.
[4] 向钱,李宁. 食品致敏性研究进展[J]. 国外医学卫生学分册,2006,33(6):376~380.
[5] Joseph P,Strain G,Ebel F. Application regarding ginkgo biloba[J]. Journal of the American Dietetic Association,2007,107(9):1 492~1 492.
[6] Bjrn M Hausen. The sensitizing capacity of ginkgolic acids in guinea pigs[J]. American Journal of Contact Dermatitis,1998,9(3):146~148.
[7] 李欣,高金燕,陈红兵. 水牛奶中β-乳球蛋白过敏原表位定位的初步研究[J]. 食品科学,2007,28(10):360~363.
[8] 杜若源,谢晶,王婷,等.超声波辅助提取银杏叶总黄酮的工艺优化[J].食品与机械,2015,31(1):167~170
[9] De Luis Ruth, Mata Luis, Estopanan Gloria. Evaluation of indirect competitive and double antibody sandwich ELISA tests to determine-lactoglobulin and ovomucoid in model processed foods[J]. Food and Agricultural Immunlilgy, 2008(19): 339~350.
[10] Hamid Reza Nouri,Danial Afsharzadeh, Abdolreza Varasteh. Diagnosis of Chenopodium album allergy with a cocktail of recombinant allergens as a tool for component-resolved diagnosis[J]. Mol Biol Rep, 2012(39): 3 169~3 178.