Determination of β-lactams by ultra performance liquid chromatography-tandem mass spectrometry with QuEChERS in goat milk

Authors

WANG Shuaishuai, ShangHai Ocean University, Shanghai 201306, China; ShangHai Bino Testing Service co., LTD, Shanghai 200436, China
ZHENG Xiaoping, ShangHai Bino Testing Service co., LTD, Shanghai 200436, China
MENG Jin, ShangHai Bino Testing Service co., LTD, Shanghai 200436, China
HE Yabin, ShangHai Bino Testing Service co., LTD, Shanghai 200436, China

Abstract

A method was developed for simultaneous determination of 8 β-lactams in goat milk by QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry (UPLC—MS/MS). The milk was extracted with acetonitrile, purified with C₁₈ and PSA sorbent and separated by an ACQUITY UPLC BEH C₁₈ column using water (containing 0.1% formic acid) and acetonitrile as mobile phase. The mass spectrometric acquisitions were carried out by means of multiple reaction monitoring (MRM) in positive ionization mode. The linear relation of eight β-lactams was good and the correlation coefficient was more than 0.986. The recovery rates of the target analyte were between 83.8%～95.4%. The relative standard deviations were less than 6.5%. The limit of detection (LOD) of 8 β-lactams was 0.25～1.00 μg/kg and the limit of quantification (LOQ) was 1.0～2.0 μg/kg. This method was reliable for the determination of β-lactams in goat milk and other dietary products.

Publication Date

6-28-2015

First Page

66

Last Page

69

DOI

10.13652/j.issn.1003-5788.2015.03.016

Recommended Citation

Shuaishuai, WANG; Xiaoping, ZHENG; Jin, MENG; and Yabin, HE (2015) "Determination of β-lactams by ultra performance liquid chromatography-tandem mass spectrometry with QuEChERS in goat milk," Food and Machinery: Vol. 31: Iss. 3, Article 16.
DOI: 10.13652/j.issn.1003-5788.2015.03.016
Available at: https://www.ifoodmm.cn/journal/vol31/iss3/16

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