Abstract
Probiotic feed yeasts were made from the raw materials of defatted soy meal, rice hull and corn straw by solid state fermentation. The culture medium ingredients were optimized by the orthogonal experiment. The model of fermentative condition was developed by the simplex-centrioid mixture design and analyzed by the response surface analysis.The results were as follows: defatted soymeal∶rice hull∶corn straw was in the ratio 5∶2∶2. Forty grams of the raw materials were added with 4 grams of glucose,0.2 grams of (NH4)2SO4 and 1× mono-microelement combination. The inoculum volum was 3.5%. The culture thickness was 1.3 cm. The ratio of water addition to raw materials was 1∶1(m/m). The fermentation temperature was 30.6 ℃ and the fermentation period was 28 h. Under the above conditions, living yeast cells was obtained at (2.92±0.05)×109 CFU/g.
Publication Date
6-28-2015
First Page
202
Last Page
207
DOI
10.13652/j.issn.1003-5788.2015.03.047
Recommended Citation
Su, XU; Libin, SUN; Haiming, YANG; Hui, SUN; and Lianzhou, JIANG
(2015)
"Process optimization of high-density Candida utilis feed yeasts by solid state fermentation,"
Food and Machinery: Vol. 31:
Iss.
3, Article 47.
DOI: 10.13652/j.issn.1003-5788.2015.03.047
Available at:
https://www.ifoodmm.cn/journal/vol31/iss3/47
References
[1] 胡学智,王俊.益生菌饲料的现状、生产和应用效果[J].中国微生态学杂志,2011,23(9):861~864.
[2] Melanie Le Bon,Helen E Davies,Caitriona Glynn,et al. Influence of probiotics on gut health in the weaned pig[J].Livestock Science,2010(133):179~181.
[3] Francesca Gaggìa,Paola Mattarelli,Bruno Biavati. Probiotics and prebiotics in animal feeding for safe food production[J].International Journal of Food Microbiology,2010(141):S15~S28.
[4] Choi J Y, Shinde P L, Ingale S L, et al. Evaluation of multi-microbe probiotics prepared by submerged liquid or solid substrate fermentation and antibiotics in weaning pigs[J]. Livestock Science,2011(138):144~151.
[5] M Ibrahim Rajoka,Sohail Hassan Khan,M A Jabbar,et al. Kinetics of batch single cell protein production from rice polishings with Candida utilis in continuously aerated tank reactors[J].Bioresource Technology,2006(97):1 934~1 941.
[6] 李永凯,毛胜勇,朱伟云. 益生菌发酵饲料研究及应用现状[J]. 畜牧与兽医,2009,41(3):90~92.
[7] 陈洪章,徐建.现代固态发酵原理与应用[M].北京:化学工业出版社,2004:38.
[8] 陶发琴,王明鹏,王卫星,等.响应面法优化酵母油脂的提取工艺[J].中国粮油学报,2013,28(4):51~58.
[9] Guo Hong-wei,Chang Juan,Yin Qing-qiang,et al.Effect of the combined physical and chemical treatments with microbial fermentation on corn straw degradation[J].Bioresource Technology,2013(148):361~365.
[10] 杨世平,刘慧玲,刘付文. 沼泽生红冬孢酵母的固体培养条件[J]. 广东海洋大学学报,2013,33(3):22~26.
[11] Gélinas P, Barrette J.Protein enrichment of potato processing waste through yeast fermentation [J].Bioresource Technology,2007(98):1 138~1 143.