Determination of α-solanine and α-chaconine in potato by HPLC—ELSD

Authors

SHANG Tingting, College of Horticulture and Landscape, Hunan Agricultural University, Changsha, Hunan 410128, China
KUANG Mengting, College of Horticulture and Landscape, Hunan Agricultural University, Changsha, Hunan 410128, China
HU Xingxi, College of Horticulture and Landscape, Hunan Agricultural University, Changsha, Hunan 410128, China; Hunan Provincial Engineering Research Center for Potatoes, Changsha, Hunan 410128, China
XIONG Xingyao, College of Horticulture and Landscape, Hunan Agricultural University, Changsha, Hunan 410128, China; Hunan Provincial Engineering Research Center for Potatoes, Changsha, Hunan 410128, China
LU Ying, College of Horticulture and Landscape, Hunan Agricultural University, Changsha, Hunan 410128, China;National Research Center of Engineering Technology for Utilization of Functional Ingredients From Botanicals, Changsha, Hunan 410128, China

Abstract

A method of reversed-phase high performance liquid chromatography (HPLC) coupled with evaporative light scattering detection (ELSD) was developed for the determination of α-solanine and α-chaconine in potato，and determine the contents in different samples. The results showed α- solanine and α- chaconine can be obtained good separation and high efficiency peaks when took 0.1% trifluoroacetic acid water and acetonitrile as mobile phase with gradient elution, and the drift tube of ELSD detector was 98 ℃, the pressure of nebulizing gas was 6.90 kPa. α-solanine and α-chaconine could be extracted with 100 times the mass 1% formic acid in methanol at 70 ℃ with 90 min, the extract liquid was injected into the chromatograph for testing directly. The linear calibration curve of α- solanine was obtained in the ranges of 0.196～9.800 μg (R²=0.999 5), the average recovery was 99.4%, the contents of α- solanine and α-chaconine be calculated with α- solanine standard curve simultaneously; There were different alkaloids contains in different parts of the potato plant, α-solanine contents were 0.13～1.59 mg/g and α-chaconine contents were 0.39～8.28 mg/g; total alkaloids were 0.52～9.37 mg/g, α-chaconine contents were 1.2 to 7.6 times of α- solanine contents in the same sample。

Publication Date

8-28-2015

First Page

55

Last Page

58,125

DOI

10.13652/j.issn.1003-5788.2015.04.014

Recommended Citation

Tingting, SHANG; Mengting, KUANG; Xingxi, HU; Xingyao, XIONG; and Ying, LU (2015) "Determination of α-solanine and α-chaconine in potato by HPLC—ELSD," Food and Machinery: Vol. 31: Iss. 4, Article 14.
DOI: 10.13652/j.issn.1003-5788.2015.04.014
Available at: https://www.ifoodmm.cn/journal/vol31/iss4/14

References

[1] Mendel Friedman,Carol E Levin. α-Tomatine content in tomato and tomato products determined by hplc with pulsed amperometric detection［J］. J. Agric. Food Chem., 1995,43(6): 1 507～1 511.
[2] Friedman M.Analysis of biologically active compounds in potatoes (Solanum tuberosum), tomatoes (Lycopersicon esculentum), and jimson weed (Datura stramonium) seeds［J］. J. Chromatogr A,2004,1 054(1～2):143～155.
[3] Mendel Friedman. Tomato glycoalkaloids: role in the plant and in the diet［J］. J. Agric. Food Chem., 2002, 50(21):5 751～5 780.
[4] Y O Son, J Kim, J C Lim, et al. Ripe fruits of solanum nigrium L. inhibits cell growth and induces apoptosis MCF-7 cells［J］. Food and Chemical Toxicology, 2003,41(10):1 421～1 428.
[5] Rajananthanan P, Attard GS, Sheikh NA, et al. Novel aggregate structure adjuvants modulate lymphocyte proliferation and Th1 and Th2 cytokine profiles in ovalbumin immunized mice［J］.Vaccine, 1999,18(1～2):140～152.
[6] Heal KG, Sheikh NA, Hollingdale MR, et al. Potentiation by a novel alkaloid glycoside adjuvant of a protective cytotoxic T cell immune response specific for a preerythrocytic malaria vaccine candidate antigen ［J］.Vaccine, 2001,19(30):4 153～4 161.
[7] Bergers W W, Alink G M. Toxic effect of the glycoalkaloids solanine and tomatine on cultured neonatal rat heart cells［J］.Toxicol.Lett.,1980, 6(1): 29～32.
[8] Thome H V,Clarke G F,Skuce R. The inactivation of herpes simplex virus by some Solanaceae glycoalkaloids［J］.Antiviral Res.,1985,5(6):335～343.
[9] M Friedman,T E Fitch, W E Yokoyama. Lowering of plasma LDL cholesterol in hamsters by the tomato glycoalkaloid tomatine［J］.Food Chemical Toxicology, 2000,38(7):549～553.
[10] Alison M Fewell, James G Roddick. Interactive antifungal activity of the glycoalkaloids α-solanine and α-chaconine［J］. Phytochemistry,1993,33(2):323～328.
[11] 王明娟,胡昌勤,金少鸿. 氨基糖苷类抗生素在蒸发光散射检测器中响应因子的一致性考察［J］.药学学报,2002,37(3):204～206.
[12] 洪建文,胡昌勤,盛龙生. 喹诺酮类抗生素在蒸发光散射检测器中响应因子的一致性考察［J］.药学学报,2003,38(9):695～697.
[13] Fang L, Wan M, Pennacchio M, et al.Evaluation of evaporative light-scattering detector for combinatorial library quantitation by reversed phase HPLC［J］.J. Comb Chem.,2000,2(3):254～257.
[14] Robert J Houben, Kommer Brunt. Determination of glycoalkaloids in potato tubers by reversed high-performance liquid chromatography［J］. Journal of Chromatography A,1994,661(1～2):169～174.
[15] 王守兰,朱佳.高效液相色谱法测定马铃薯中α-茄碱的含量［J］.食品科学,1996,17(6):60～61.
[16] 肖文,李勤,熊兴耀,等.高效液相色谱法分析马铃薯中α-茄碱［J］.分析化学,2011,39(9):1 459～1 460.
[17] Lan Dao, Mendel Friedman. Comparison of glycoalkaloid content of fresh and freeze-dried potato leaves determined by HPLC and colormetry［J］. Agric. Food Chem.,1996(8):2 287～2 291.
[18] Sotelo A, Serrano B.High-performance liquid chromatographic determination of the glycoalkaloids alpha-solanine and alpha-chaconine in 12 commercial varieties of Mexican potato ［J］. Agric. Food Chem.,2000,48(6):2 472～2 475.