Optimization on culture medium for laccase production from Coriolus hirsutus by residues of traditional Chinese medicine

Authors

YUE Kun, Tianjin Modern Vocation Technology College, Tianjin 300350, China
PAN Zhiheng, Tianjin Modern Vocation Technology College, Tianjin 300350, China
SUN Yongmin, Tianjin Modern Vocation Technology College, Tianjin 300350, China

Abstract

In order to make full use of residue from traditional Chinese medicine, the different kinds of residues of traditional Chinese Medicine for laccase production by Coriolus hirsutus were investigated. It is capable of producing laccase with the three kinds of residues of traditional Chinese Medicine. The result showed that the optimal medium composition was as follows: wheat bran 3%, peptone 02%, residues of Chinese rhubarb 12%, MgSO₄ 3 g/L，KH₂PO₄ 3 g/L, pH value 5.0. Enzyme activity under the optimal culture conditions was 4 828.2 U/mL, which was 1.4 fold of that of the original medium.

Publication Date

10-28-2015

First Page

47

Last Page

50

DOI

10.13652/j.issn.1003-5788.2015.05.011

Recommended Citation

Kun, YUE; Zhiheng, PAN; and Yongmin, SUN (2015) "Optimization on culture medium for laccase production from Coriolus hirsutus by residues of traditional Chinese medicine," Food and Machinery: Vol. 31: Iss. 5, Article 11.
DOI: 10.13652/j.issn.1003-5788.2015.05.011
Available at: https://www.ifoodmm.cn/journal/vol31/iss5/11

References

[1] 司静, 李伟, 崔宝凯, 等. 真菌漆酶性质、分子生物学及其应用研究进展［J］. 生物技术通报, 2011(2): 48～55.
[2] 刘忠川, 王刚刚. 真菌漆酶结构与功能研究进展［J］. 生物物理学报, 2013, 29(9): 629～645.
[3] 王锋, 刘英, 汪印, 等. 漆酶及其应用［J］. 生物加工过程, 2012, 10(4): 70～76.
[4] 赵玉萍, 陈晓旺. 产漆酶培养基条件的优化［J］. 食品与机械, 2012, 28(6): 226～230.
[5] Liu Zhi-yu, Zhang Dong-xu, Hua Zhao-zhe, et al. Improvement of laccase production and its properties by low-energy ion implantation［J］. Bioprocess and Biosystems Engineering, 2010, 33(5): 639～646.
[6] 刘海新, 马浩, 黄海娟, 等. 中药药渣的综合利用研究进展［J］. 发酵科技通讯, 2014, 43(1): 30～32.
[7] 杨丽红, 叶选怡, 凌庆枝, 等. 中药渣固体发酵亮菌产漆酶研究［J］. 安徽农业科学, 2013, 41（6）: 2 396～2 398.
[8] 朱友双. 杂色云芝液态和固态发酵产漆酶及离子液体对生物质预处理的研究［D］. 济南: 山东大学, 2011.
[9] 李慧蓉. 白腐真菌生物学和生物技术［M］. 北京: 化学工业出版社, 2005: 78～83.
[10] 林俊芳, 刘志明, 陈晓阳, 等. 真菌漆酶的酶活测定方法评价［J］. 生物加工过程, 2009, 7(4): 1～8.
[11] 朱雄伟, 胡道伟, 梅运军, 等. 通过碳源与氮源研究白腐菌产漆酶和菌丝体生长的相关性［J］. 微生物学杂志, 2003, 23(3): 12～14.
[12] 刘家扬, 蔡宇杰, 廖祥儒, 等. 漆酶高产菌的筛选及产酶优化［J］. 食品与机械, 2010, 26(4): 10～14.
[13] 刘华钢, 申庆荣, 刘丽敏. 中药抗菌研究进展［J］. 时珍国医国药, 2010, 21(2): 215～217.
[14] 赵亮亮. 红芝发酵产漆酶及其在染料脱色中的应用研究［D］. 桂林: 广西师范大学, 2010.
[15] 费娜, 杨俊杰. 大黄等四种中草药体外抑菌活性比较［J］. 黑龙江医药科学, 2010, 33(1): 18～19.