Abstract
To develop a loop-mediated isothermal amplification (LAMP) assay for visual detecting Staphylococcus aureus, 2 sets of specific primers were designed and 6 primers targeting the nuc gene was selected. The method could detect Staphylococcus aureus within 45 min at a constant temperature of 63 ℃. The sensitivity of the method was 1 pg/μL, and had no cross reaction with other pathogens. The result indicated that the LAMP assay was a rapid, specific and simple tool for field diagnosis of Staphylococcus aureus.
Publication Date
10-28-2015
First Page
55
Last Page
58
DOI
10.13652/j.issn.1003-5788.2015.05.013
Recommended Citation
Taoping, SONG; Huali, QIU; Shujuan, WANG; Xinkai, PENG; and Lixia, YANG
(2015)
"Development of a loop-mediated isothermal amplification assay for visual detection of Staphylococcus aureus,"
Food and Machinery: Vol. 31:
Iss.
5, Article 13.
DOI: 10.13652/j.issn.1003-5788.2015.05.013
Available at:
https://www.ifoodmm.cn/journal/vol31/iss5/13
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