Separation, monosaccharide composition and molecular weight analysis of polysaccharide from G. lucidum mycelium

Authors

WANG Haiyan, Food Academy of Jiangnan University, Wuxi, Jiangsu 214022, China
DAI Jun, Food Academy of Jiangnan University, Wuxi, Jiangsu 214022, China
CHEN Shangwei, State Key Laboratory of Food Science and Technology in Jiangnan University, Wuxi, Jiangsu 214022, China

Abstract

The polysaccharide was extracted from G. lucidum mycelium by submerged fermentation after previous hybird optimization. The polysaccharide of G. lucidum mycelium was separated by DEAE Sepharose fast flow column. Then we used high volume exclusion chromatography (HPSEC) to explore purity of three polysaccharide fractions and complete acid hydrolysis and PMP pre column derivatization RP—HPLC to measure the monosaccharide composition of them. At last, multi angle light scattering instrument combined with device (SEC—MALLS) was used to measure the Mw of polysaccharide fractions, while the slope of curves between the molecular rotation radius and molecular molar of moles inferred the space conformation of them. HPSEC results showed that the purified of GLMP1, GLMP2 and GLMP3 were 93.58%, 97.64% and 99.19%. Monosaccharide composition analysis showed that mannose, rhamnose, galacturonic acid, glucose, galactose, xylose, arabinose and fucose were contained in GLMP1, GLMP2 and GLMP3, however they had different molar ratios of monosaccharide. SEC—MALLS tested the absolute weight-average molecular weights (Mw) of GLMP1, GLMP2 and GLMP3 respectively were 4.526×10⁵, 4.603×10⁴ and 3.760×10³ g/mol and it suggested that the conformations of the three polysaccharide fractions maybe were polymers which were high tightening and having branched structures.

Publication Date

10-28-2015

First Page

201

Last Page

205

DOI

10.13652/j.issn.1003-5788.2015.05.052

Recommended Citation

Haiyan, WANG; Jun, DAI; and Shangwei, CHEN (2015) "Separation, monosaccharide composition and molecular weight analysis of polysaccharide from G. lucidum mycelium," Food and Machinery: Vol. 31: Iss. 5, Article 52.
DOI: 10.13652/j.issn.1003-5788.2015.05.052
Available at: https://www.ifoodmm.cn/journal/vol31/iss5/52

References

[1] 毛健, 马海乐. 灵芝多糖的研究进展［J］. 食品科学, 2010, 31(1): 295~299.
[2] Shi Min, Zhang Zhen-ya, Yang Ying-nan. Antioxidant and immunoregulatory activity of Ganoderma lucidum polysaccharide (GLP)［J］. Carbohydrate Polymers, 2013, 95(1): 200~206.
[3] Jia Jie, Zhang Xi, Hu Yong-shan, et al. Evaluation of in vivo antioxidant activities of Ganoderma lucidum polysaccharides in STZ-diabetic rats［J］. Food Chemistry, 2009, 115(1): 32~36.
[4] Yang Qian, Wang Si-wang, Xie Yan-hua, et al. HPLC analysis of Ganoderma lucidum polysaccharides and its effect on antioxidant enzymes activity and Bax, Bcl-2 expression［J］. International Journal of Biological Macromolecules, 2010, 46(2): 167~172.
[5] Seto S W, Lam T Y, Tam H L, et al. Novel hypoglycemic effects of Ganoderma lucidum water-extract in obese/diabetic (+ db/+ db) mice［J］. Phytomedicine, 2009, 16(5): 426~436.
[6] 衣艳君, 徐承水. 灵芝降血脂作用的实验研究［J］. 安徽师范大学报, 2001, 24(1): 52~53.
[7] Peng Yan-fei, Zhang Li-na, Zeng Fan-bo, et al. Structure and antitumor activities of the water-soluble polysaccharides from Ganoderma tsugae mycelium［J］. Carbohydrate Polymers, 2005, 59(3): 385~392.
[8] Boh B, Berovic M, Zhang Jing-song, et al. Ganoderma lucidum and its pharmaceutically active compounds［J］. Biotechnology Annual Review, 2007(13): 265~301.
[9] 李云, 曾东方. 食用菌液体深层发酵的研究热点［J］. 食品工业科技, 2006, 27(7): 198~201.
[10] 贺红. 灵芝液体深层发酵技术研究进展及展望［J］. 基层中药杂志, 2000, 14(2): 48~49.
[11] Nie Shao-ping, Zhang Hui, Li Wen-juan, et al. Current development of polysaccharides from Ganoderma: Isolation, structure and bioactivities［J］. Bioactive Carbohydrates and Dietary Fibre, 2013, 1(1): 10~20.
[12] 毛健, 马海乐. 灵芝多糖的研究进展［J］. 食品科学, 2010(1): 295~299.
[13] 张惟杰 糖复合物生化研究技术［M］. 浙江: 浙江大学出版社, 1994: 32,89,256.
[14] 孙小梅, 戴军, 陈尚卫, 等. 灵芝子实体多糖提取方法优化及不同来源赤芝子实体的多糖分子质量比较［J］. 食品与发酵工业, 2014, 40(7): 212~217.
[15] 方积年. 多糖的分离纯化及其纯度鉴别与分子量测定［J］. 药学通报, 1984, 19(10): 46~49.
[16] Ferreira I C F R, Heleno S A, Reis F S, et al. Chemical features of Ganoderma polysaccharides with antioxidant, antitumor and antimicrobial activities［J］. Phytochemistry, 2014, 10(11): 3~17.
[17] Wang Jun-long, Guo Hong-yun, Zhang Ji, et al. Sulfated modification, characterization and structure antioxidant relationships of Artemisia sphaerocephala polysaccharides［J］. Carbohydrate Polymers, 2010, 81(4): 897~905.
[18] Li Yan-qun, Fang Lu, Zhang Ke-chang. Structure and bioactivities of a galactose rich extracellular polysaccharide from submergedly cultured Ganoderma lucidum［J］. Carbohydrate Polymers, 2007, 68(2): 323~328.
[19] Wyatt P J. Light scattering and the absolute characterization of macromolecules［J］. Analytica chimica acta, 1993, 272(1): 1~40.
[20] 罗立新，周少奇，姚汝华. 灵芝多糖的结构分析［J］.分析实验室，1998, 17(4): 17~21.