Extraction of animal-derived DNA in processed food and establishment of multiplex PCR method

Authors

HE Haining, Quality and Safety Technological Innovation Service Platform on Circulation of Animal Origin, Gansu Institute of Business and Technology, Lanzhou, Gansu 730010, China; Key Laboratory of Animal Products Safety Analysis and Detection Technology of Gansu Pro
HONG Xia, Quality and Safety Technological Innovation Service Platform on Circulation of Animal Origin, Gansu Institute of Business and Technology, Lanzhou, Gansu 730010, China; Key Laboratory of Animal Products Safety Analysis and Detection Technology of Gansu Pro
FENG Yusheng, Quality and Safety Technological Innovation Service Platform on Circulation of Animal Origin, Gansu Institute of Business and Technology, Lanzhou, Gansu 730010, China; Key Laboratory of Animal Products Safety Analysis and Detection Technology of Gansu Pro
WANG Yongsong, Quality and Safety Technological Innovation Service Platform on Circulation of Animal Origin, Gansu Institute of Business and Technology, Lanzhou, Gansu 730010, China; Key Laboratory of Animal Products Safety Analysis and Detection Technology of Gansu Pro
JIN Ying, Quality and Safety Technological Innovation Service Platform on Circulation of Animal Origin, Gansu Institute of Business and Technology, Lanzhou, Gansu 730010, China; Key Laboratory of Animal Products Safety Analysis and Detection Technology of Gansu Pro
YUAN Caixia, Quality and Safety Technological Innovation Service Platform on Circulation of Animal Origin, Gansu Institute of Business and Technology, Lanzhou, Gansu 730010, China; Key Laboratory of Animal Products Safety Analysis and Detection Technology of Gansu Pro
QIAN Yingwen, Quality and Safety Technological Innovation Service Platform on Circulation of Animal Origin, Gansu Institute of Business and Technology, Lanzhou, Gansu 730010, China; Key Laboratory of Animal Products Safety Analysis and Detection Technology of Gansu Pro
LIU Qi, Quality and Safety Technological Innovation Service Platform on Circulation of Animal Origin, Gansu Institute of Business and Technology, Lanzhou, Gansu 730010, China; Key Laboratory of Animal Products Safety Analysis and Detection Technology of Gansu Pro
GAO Zhiying, Quality and Safety Technological Innovation Service Platform on Circulation of Animal Origin, Gansu Institute of Business and Technology, Lanzhou, Gansu 730010, China; Key Laboratory of Animal Products Safety Analysis and Detection Technology of Gansu Pro

Abstract

In order to get high quantity and quality animal-derived DNA extraction from processed food, the CTAB method was improved for the PCR test. According to duck mitochondrial gene sequences, primers were designed and synthesized for detecting PCR system, optimizing reaction conditions and screening, establishing a triple PCR method. The amplified lengths of fragments were 116 bp for bovine, 212 bp for porcine, 322 bp for duck, respectively. The triple PCR assay to distinguish pork and duck from beef was developed. Lower amounts of meat than declared on the label. These practices are of great importance in the fight of forged food, examining the food labels, protecting the right to know and the health concern of consumers.

Publication Date

12-28-2015

First Page

79

Last Page

83

DOI

10.13652/j.issn.1003-5788.2015.06.017

Recommended Citation

Haining, HE; Xia, HONG; Yusheng, FENG; Yongsong, WANG; Ying, JIN; Caixia, YUAN; Yingwen, QIAN; Qi, LIU; and Zhiying, GAO (2015) "Extraction of animal-derived DNA in processed food and establishment of multiplex PCR method," Food and Machinery: Vol. 31: Iss. 6, Article 17.
DOI: 10.13652/j.issn.1003-5788.2015.06.017
Available at: https://www.ifoodmm.cn/journal/vol31/iss6/17

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