Studies on extraction and molecular properties of Gualou seed (Semen Trichosanthis) protein prepared from by-products

Authors

LI Xianghong, Department of Food and Biological Engineering, Changsha University of Science and Technology, Changsha, Hunan 410114, China
LI Min, Department of Food and Biological Engineering, Changsha University of Science and Technology, Changsha, Hunan 410114, China
LIU Yongle, Department of Food and Biological Engineering, Changsha University of Science and Technology, Changsha, Hunan 410114, China
YU Jian, Department of Food and Biological Engineering, Changsha University of Science and Technology, Changsha, Hunan 410114, China
WANG Faxiang, Department of Food and Biological Engineering, Changsha University of Science and Technology, Changsha, Hunan 410114, China
WANG Jianhui, Department of Food and Biological Engineering, Changsha University of Science and Technology, Changsha, Hunan 410114, China

Abstract

In present study, Gualou seed proteins were extracted from its by-products, and the molecular properties of the proteins were also observed. The results showed that the Gualou seed proteins mainly consisted of salt-soluble protein (55.3% of extracting yield) and alkali-soluble protein (15.8% of extracting yield), and the alkali-soluble protein may be linked with pigments because the protein solution appeared to be dark brown. Sodium dodecyl sulfonate-polyacrylamide gelelectrophoresis analysis was used to test the subunit molecular weight distribution and the result revealed that subunits of salt-soluble and alkali-soluble proteins mainly distributed from 10 to 32 kDa. The molecular weight distribution of the samples, observed by size exclusion chromatography showed that there were more fractions with large molecules larger than 10⁶ Da in alkali-soluble protein. Microstructural observation by scanning electron microscopy displayed that the salt-soluble proteins were globular proteins with size of 2～5 μm, while alkali-soluble protein were aggregates with compact structure.

Publication Date

12-28-2015

First Page

146

Last Page

149

DOI

10.13652/j.issn.1003-5788.2015.06.034

Recommended Citation

Xianghong, LI; Min, LI; Yongle, LIU; Jian, YU; Faxiang, WANG; and Jianhui, WANG (2015) "Studies on extraction and molecular properties of Gualou seed (Semen Trichosanthis) protein prepared from by-products," Food and Machinery: Vol. 31: Iss. 6, Article 35.
DOI: 10.13652/j.issn.1003-5788.2015.06.034
Available at: https://www.ifoodmm.cn/journal/vol31/iss6/35

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