Effects of breaking cell wall on rape bee pollen treated with pectinase using multi-index evaluation

Authors

WANG Kai, College of Food Engineering, Harbin University of Commerce, Harbin, Heilongjiang 150076, China; Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China; National Research Centre for Bee Product Processing, Beijin
REN Xiangnan, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China; National Research Centre for Bee Product Processing, Beijing 100093, China
DONG Jie, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China; National Research Centre for Bee Product Processing, Beijing 100093, China
ZHANG Gensheng, College of Food Engineering, Harbin University of Commerce, Harbin, Heilongjiang 150076, China

Abstract

To improve wall-broken effects of bee pollen, rape bee pollen was selected as material and treated with pectinase in different pH and temperature. The effects of pectinase on rape bee pollen were evaluated by protein dispersion index, soluble sugar content, microscopic analysis and particle size distribution. The results demonstrated that pectinase hydrolysis could disintegrate the intine at the germinal apertures, the optimal conditions were pH 3.5 and temperature 50 ℃. Under optimal conditions, protein dispersion index was 0.684 6, and soluble sugar content was 24.86%. By microscopic analysis, some germinal apertures seemed apart and some little particles emerged. According to particle size distribution, the holes area of pollen increased 39.20%, and distribution of pollen grains shifted towards higher holes ratio. At the same time, the particle size distribution shifted towards smaller size of particles and the number of particles in small sizes was increasing, in particular, fragments below 1 μm increased by 339.92%.

Publication Date

12-28-2015

First Page

164

Last Page

168

DOI

10.13652/j.issn.1003-5788.2015.06.039

Recommended Citation

Kai, WANG; Xiangnan, REN; Jie, DONG; and Gensheng, ZHANG (2015) "Effects of breaking cell wall on rape bee pollen treated with pectinase using multi-index evaluation," Food and Machinery: Vol. 31: Iss. 6, Article 39.
DOI: 10.13652/j.issn.1003-5788.2015.06.039
Available at: https://www.ifoodmm.cn/journal/vol31/iss6/39

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