Determination of diethylstilbestrol in microbial degradation systems by HPLC—UV

Authors

DENG Weiqin, College of Food Science, Sichuan Agricultural University, Ya’an, Sichuan 625014, China
LI Ashuang, College of Food Science, Sichuan Agricultural University, Ya’an, Sichuan 625014, China
PENG Xian, College of Food Science, Sichuan Agricultural University, Ya’an, Sichuan 625014, China
LIU Shuangshuang, College of Food Science, Sichuan Agricultural University, Ya’an, Sichuan 625014, China
HU Kaidi, College of Food Science, Sichuan Agricultural University, Ya’an, Sichuan 625014, China
ZHANG Mengmei, College of Food Science, Sichuan Agricultural University, Ya’an, Sichuan 625014, China
HE Li, College of Food Science, Sichuan Agricultural University, Ya’an, Sichuan 625014, China
CHEN Shujuan, College of Food Science, Sichuan Agricultural University, Ya’an, Sichuan 625014, China
HAN Xinfeng, College of Food Science, Sichuan Agricultural University, Ya’an, Sichuan 625014, China
LIU Shuliang, College of Food Science, Sichuan Agricultural University, Ya’an, Sichuan 625014, China

Abstract

The concentration of diethylstilbestrol (DES) in the microbial degradation systems was determined using HPLC—UV. Different extraction methods were used in the fungi and bacteria fermentation systems, DES was separated through a Sepax GP-C₁₈ (150 mm×4.60 mm, 5.0 μm), eluted with methanol-water (7︰3, V/V) at 0.7 mL/min, and detected at 242 nm. Results showed that retention time of DES was 7.714 min. Good linearity was obtained with the detection limits of 0.5～50.0 mg/L with a correlation coeffcient of 0.996 3. The average spike recovers of DES for bacteria and fungi fermentation systems were 94.04% and 95.24%, respectively, with RSD of 2.33% and 1.05%, respectively. The limits of detection was not more than 0.30 mg/L. Results showed that the ratios of DES (25 mg/L) degration of four strains could degrade 62.38%, 64.72%, 53.38%, 88.34% , respectively in 5 d. The developed method presented the benefits of simplicity, rapidity, good separation, high accuracy, high precision and good stability.

Publication Date

1-28-2016

First Page

51

Last Page

55

DOI

10.13652/j.issn.1003-5788.2016.01.012

Recommended Citation

Weiqin, DENG; Ashuang, LI; Xian, PENG; Shuangshuang, LIU; Kaidi, HU; Mengmei, ZHANG; Li, HE; Shujuan, CHEN; Xinfeng, HAN; and Shuliang, LIU (2016) "Determination of diethylstilbestrol in microbial degradation systems by HPLC—UV," Food and Machinery: Vol. 32: Iss. 1, Article 12.
DOI: 10.13652/j.issn.1003-5788.2016.01.012
Available at: https://www.ifoodmm.cn/journal/vol32/iss1/12

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