Abstract
The effects was studied on the biologically active substances content and antioxidant activity of G. procumbens extracts, which was from different parts of Gynura procumbens(Lour.) Merr, by different drying methods, and with different kinds of solvents. The results showed that: G. procumbens roots compared to its stems and leaves had higher content of phenolic and flavonoids and the antioxidant activity was also stronger, and the ferric reducing ability and ABTS+·scavenging capacity and DPPH·scavenging capacity were 21.25±0.31, 35.27±0.26, 56.20±0.22 TEAC（trolox equivalent antioxidant capacity）value (μg Trolox/mL), respectively. Drying methods had significant effect on the content of the biologically active substance of G. procumbens roots extracts, freeze drying and 60 ℃ hot air drying obtained with strong antioxidant activity. Extraction solvents had significant effect on the content of the biologically active substance of G. procumbens roots extracts, and the antioxidant activity (FRAP ferric reducing ability, DPPH·scavenging capacity and ABTS+·scavenging capacity) of extracts and their total phenolic content were significantly correlated, with the correlation coefficient R of 0.919, 0.848 and 0.907, respectively. The antioxidant activity (FRAP, DPPH and ABTS) of extracts and their total flavonoids content were significantly correlated, too, with the correlation coefficient R of 0.915, 0.793 and 0.823, respectively. The antioxidant activity (FRAP, DPPH and ABTS) and chlorogenic acid content correlation coefficient R were 0.887, 0.927 and 0.900, respectively, but had less relevant with the vitamin C content was.
Publication Date
10-28-2016
First Page
145
Last Page
150
DOI
10.13652/j.issn.1003-5788.2016.10.035
Recommended Citation
Xing, MENG; Anping, LI; Jiangfan, YU; and Dunyuan, HUANG
(2016)
"Study on vitro-antioxidant activities of extracts of Gynura procumbens(Lour.) merr,"
Food and Machinery: Vol. 32:
Iss.
10, Article 34.
DOI: 10.13652/j.issn.1003-5788.2016.10.035
Available at:
https://www.ifoodmm.cn/journal/vol32/iss10/34
References
［1］ 中国科学院《中国植物志》编辑委员会. 中国植物志: 第77卷 第一分册［M］. 北京: 科学出版社, 2010: 322.
［2］ KIM J, LEE C W, KIM E K, et al. Inhibition effect of Gynura procumbens extract on UV-B-induced matrix-metalloproteinase expression in human dermal fibroblasts［J］. Journal of Ethnopharmacology, 2011, 137(1): 427-33.
［3］ KIM M J, LEE H J, WIRYOWIDAGDO S, et al. Antihypertensive efects of Gynura procumbens extract in spontaneously hypertensive rats［J］. Journal of Medicinal Food, 2006, 9(4): 587-590.
［4］ HUANG Wu-yang, CAI Yi-zhong, ZHANG Yan-bo. Natural phenolic compounds from medicinal herbs and dietary plants: potential use for cancer prevention［J］. Nutrition & Cancer, 2010, 62(1): 1-20.
［5］ 钟琼芯. 海南岛菊三七属药用植物资源调查［J］. 海南师范大学学报: 自然科学版, 2001, 14(2): 42-44.
［7］ PUANGPRONPITAG D, CHAICHANADEE S, SITTIWET C, et al. Evaluation of nutritional value and antioxidative properties oh the medicinal plant gynura procumbens extractf［J］. Asian Journal of Plant Sciences, 2010, 9(3): 146-151.
［8］ 朱玉婷, 廖为明, 郑国栋, 等. 平卧菊三七中绿原酸提取及纯化工艺的优化［J］. 湖北农业科学, 2012, 51(19): 4 348-4 351.
［9］ 叶芝红, 赵艳, 朱艳萍, 等. 响应面法优化微波辅助提取平卧菊三七三萜的工艺研究［J］. 食品工业科技, 2016, 37(2): 291-295.
［10］ 郑国栋, 林乐珍, 黎冬明. 微波辅助提取平卧菊三七中总黄酮的工艺研究［J］. 江苏农业科学, 2015(8): 279-281.
［11］ 叶芝红, 林乐珍, 熊红霞, 等. 超声波提取平卧菊三七总三萜的工艺研究［J］. 食品工业, 2016(2): 10-13.
［12］ 门凤麟, 王英锋, 祝翔. 平卧菊三七提取物降血糖实验研究［J］. 首都师范大学学报: 自然科学版, 2015, 36(2): 39-42.
［13］ 郑国栋, 钟树生, 张清峰, 等. 平卧菊三七对小鼠血糖及血脂的影响［J］. 现代食品科技, 2013(12): 2 800-2 804.
［14］ 郑国栋, 帅丽乔娃, 黎冬明, 等. 平卧菊三七提取及抗菌作用的研究［J］. 食品科技, 2014(4): 218-221.
［15］ KAEWSEEJAN N, SUTTHIKHUM V, SIRIAMORNPUN S. Potential of Gynura procumbens leaves as source of flavonoid-enriched fractions with enhanced antioxidant capacity［J］. Journal of Functional Foods, 2015, 12: 120-128.
［16］ SU Dong-xiao, ZHANG Rui-fen, HOU Fang-li, et al. Comparison of the free and bound phenolic profiles and cellular antioxidant activities of litchi pulp extracts from different solvents［J］. Bmc Complementary & Alternative Medicine, 2014, 14(1): 1-10.
［17］ ANAIDU M M, SHYAMALA B N, NAIK J P, et al. Chemical composition and antioxidant activity of the husk and endosperm of fenugreek seeds［J］. LWT - Food Science and Technology, 2011, 44(2): 451-456.
［18］ ABU BAKAR M F, MOHAMED M, RAHMAT A,et al. Phytochemicals and antioxidant activity of different parts of bambangan (Mangifera pajang) and tarap (Artocarpus odoratissimus)［J］. Food Chemistry, 2009, 113(2): 479-483.
［19］ KIM D O, JEONG S W, LEE C Y. Antioxidant capacity of phenolic phytochemicals from various cultivars of plums［J］. FoodChemistry, 2003, 81(3): 321-326.
［20］ 胡鲜宝, 吕加平, 刘鹭, 等. 葵花粕中绿原酸检测方法的建立［J］. 食品工业科技, 2011, 32(2): 341-343.
［21］ 向福, 刘亮, 秦婷, 等. 罗田金银花叶中绿原酸的提取工艺改进［J］. 食品与机械, 2013, 29(6): 150-152.
［22］ 韦献雅, 殷丽琴, 钟成, 等. DPPH法评价抗氧化活性研究进展［J］. 食品科学, 2014, 35(9): 317-322.
［23］ 孙术国, 杨涛, 林亲录, 等. 加工方式对稻米抗氧化性的影响［J］. 食品与机械, 2014, 30(6): 132-134.
［24］ KRISHNAN V, AHMAD S, MAHMOOD M. Antioxidantpotential in different parts and callus of gynura procumbens and different parts of gynura bicolor［J］. Biomed Research International, 2015(6): 1-7.
［25］ JALEEL C A. Antioxidant profile changes in leaf and root tissues ofwithania somnifera Dunal［J］. Plant Omics, 2009, 2(4): 163-168.
［26］ 尚红梅, 郭玮, 潘丹, 等. 干燥方式对菊苣根多酚含量和抗氧化活性的影响［J］. 食品科学, 2015, 36(1): 84-88.
［27］ MOHDALY A A, SARHAN M A, SMETANSKA I, et al. Antioxidant properties of various solvent extracts of potato peel, sugar beet pulp and sesame cake［J］. Journal of the Science of Food & Agriculture, 2010, 90(2): 218-226.
［28］ 金莹. 苹果多酚的超声波提取及其抗氧化性研究［D］. 泰安: 山东农业大学, 2006: 49-50.
［29］ 曹清明, 邬靖宇, 钟海雁, 等. 油茶叶中黄酮的超声辅助提取及其抗氧化活性研究［J］. 食品与机械, 2015, 31(3): 162-166.