Optimization of protease enzymatic hydrolysis of sheep lung and studies on antioxidant activities of its hydrolysates

Authors

YANG Xuhui, College of Liquor and Food, Guizhou University, Guiyang, Guizhou 550025, China
WEN Fei, College of Liquor and Food, Guizhou University, Guiyang, Guizhou 550025, China
WANG Xianqian, College of Liquor and Food, Guizhou University, Guiyang, Guizhou 550025, China
QI Qi, College of Life Sciences, Guizhou University, Guiyang, Guizhou 550025, China
SU Wei, College of Liquor and Food, Guizhou University, Guiyang, Guizhou 550025, China
TUO Ni, Qinglong County, Guizhou Sea Power Sheep Halal Food Processing Ltd., Southwest Guizhou Autonomous Prefecture, Guizhou 561400, China

Abstract

By using the hydrolysis degree as the evaluation, the sheep lung enzymatic process was optimized to investigate the antioxidate activities of the enzymolysis products, hydrolyzed by neutral protease. On the basis of single factor studies, the response surface analysis (RSM) assay was then used to obtain the best enzymatic proscess. Our results showed that the optimal condition was as follows. The enzyme amount was 1 700.0 U/g, with solid-liquid ratio 2∶1(mL/g) , enzymatically hydrolyzed for 4 h at 50 ℃. Under this optimal conditions, 3 times of the replication experiments turned out that the error of the actual degree of hydrolysis (18.89%) was small, compared with the theory one (18.19%). Moreover, the absorption value of reducing power was found to be 0.472, and IC₅₀ of the DPPH radical scavenging rate, the superoxide anion radical scavenging rate, and the hydroxyl radical scavenging rate were 67 mg/mL, 40.07 mg/mL, and 31.88 mg/mL, respectively. These results of above detection indicated that enzymatic hydrolysates of sheep lungs possessed certain antioxidant activity in virto.

Publication Date

10-28-2016

First Page

151

Last Page

157

DOI

10.13652/j.issn.1003-5788.2016.10.036

Recommended Citation

Xuhui, YANG; Fei, WEN; Xianqian, WANG; Qi, QI; Wei, SU; and Ni, TUO (2016) "Optimization of protease enzymatic hydrolysis of sheep lung and studies on antioxidant activities of its hydrolysates," Food and Machinery: Vol. 32: Iss. 10, Article 35.
DOI: 10.13652/j.issn.1003-5788.2016.10.036
Available at: https://www.ifoodmm.cn/journal/vol32/iss10/35

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