•  
  •  
 

Abstract

This review mainly focuses on the progress on the application of aptamers on food safety detection. Firstly, the nature, mechanism and characteristics of aptamers were introduced; Secondly, four types of SELEX technology from the angle of the fixed objects and the composition of library were classified; finally, the application and development of aptamers in food safety detection were reviewed and prospected.

Publication Date

10-28-2016

First Page

194

Last Page

199

DOI

10.13652/j.issn.1003-5788.2016.10.044

References

[1] 江南大学国家社科重大课题《食品安全风险社会共治研究》研究团队. 1-3月我国食品安全事件分析[N]. 中国食品安全报, 2016-5-12(A2).
[2] 满燕, 吕雪飞, 张玉奎, 等. 核酸适配体及其在生物医学研究中的应用[J]. 航天医学与医学工程, 2013, 26(6): 485-490.
[3] GOLD L. Conformational properties of oligonucleotides[J]. Nucleic Acids Symposium, 1995(33): 20-22.
[4] 杨歌, 魏强, 赵新颖, 等. 蛋白质的核酸适配体筛选的研究进展[J]. 色谱, 2016, 34(4): 370-381.
[5] 刘品多, 屈锋. 全细胞的核酸适配体筛选的研究进展[J]. 色谱, 2016(4): 382-388.
[6] 于寒松, 隋佳辰, 代佳宇, 等. 核酸适配体技术在食品重金属检测中的应用研究进展[J]. 食品科学, 2015, 36(15): 228-233.
[7] 王周平, 张维潇. 适配体及其研究进展[J]. 食品与生物技术学报, 2013, 32(9): 897-906.
[8] ELLINGTON A D, SZOSTAK J W. In vitro selection of RNA molecules that bind specific ligands[J]. Nature, 1990, 346: 818-822.
[9] HERMANN T, PATEL DJ. Biochemistry-Adaptive recognition by nucleic acid aptamer[J]. Science, 2000, 287(5 454): 820-825.
[10] 段诺. 食源性致病菌适配体的筛选及分析应用研究[D]. 无锡: 江南大学, 2013: 5.
[11] MCKEAGUE M, MCCONNELL E M, CRUZ-TOLEDO J, et al. Analysis of in vitro aptamer selection parameters[J]. Journal of Molecular Evolution, 2015, 81(5/6): 150-161.
[12] 徐发良, 肖觉. SELEX技术的靶标范围拓展[J]. 第二军医大学学报, 2012, 33(4): 432-435.
[13] TUERK C, GOLD L. Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase[J]. Science, 1990, 249: 505-510.
[14] JACOBSON O, YAN Xue-feng, NIU G, et al. PET imaging of tenascin-C with a radio labeled single-stranded DNA aptamer[J]. Journal of Nuclear Medicine, 2015, 56(4): 616-621.
[15] ZENG Zi-hua, PAREKH Parag, LI Zheng, et al. Specific and sensitive tumor imaging using biostable oligonucleotide aptamer probes[J]. Theranostics, 2014, 4(9): 945-952.
[16] LE TRINH T, ZHU Gui-zhi, XIAO Xi-lin, et al. A synthetic aptamer-drug adduct for targeted liver cancer therapy[J]. PLOS ONE, 2015, 10(11): doi:10.1371/journa.pone.0136673.
[17] NG E W M, SHIMA D T, CALIAS P, et al. Pegaptanib, a targeted anti-VEGF aptamer for ocular vascular disease[J]. Nature Reviews Drug Discovery, 2006, 5(2): 123-132.
[18] HUANG Yu-kun, CHEN Xiu-juan, WU Shi-jia, et al. Homogeneous time-resolved fluorescence assay for the detection of ricin using an aptamer immobilized on europium-doped KGdF4 nanoparticles and graphene oxide as a quencher[J]. Microchimica Acta, 2015, 182(5/6):1 035-1 043.
[19] 朱亚宝, 刘晓富, 翟丽芳, 等.细菌耐药性与抗生素的合理使用[J]. 检验医学与临床, 2010, 7(3): 286-288.
[20] NIAZI J H, LEE Su-jin, GU Man-bock. Single-stranded DNA aptamers specific for antibiotics tetracyclines[J]. Bioorganic & Medicinal Chemistry, 2008, 16(15): 7 245-7 253.
[21] STOLTENBURG R, NIKOLAUS N, STREHLITZ B. Capture-SELEX: Selection of DNA aptamers for Aminoglycoside antibiotics[J]. Journal of Analytical Methods in Chemistry, 2012(1): 155-166.
[22] KWON M, CHUN S M, JEONG S, et al. In vitro selection of RNA against kanamycin B[J]. Molecules and Celles, 2001, 11(3): 303-311.
[23] ZHOU Nan-di, WANG Jing-yuan, ZHANG Juan, et al. Selection and identification of streptomycin-specific single-stranded DNA aptamers and the application in the detection of streptomycin in honey[J]. Talanta, 2013, 108: 109-116.
[24] DUAN Nuo, WU Shi-jia, CHEN Xiu-juan, et al. Selection and characterization of aptamers against salmonella typhimurium using whole-bacterium systemic evolution of ligands by exponential enrichment (SELEX)[J]. J. Agric. Food Chem., 2013, 61: 3 229-3 234.
[25] LEE YJ, HAN SR, MAENG JS, et al. In vitro selection of Escherichia coli O157:H7-specific RNA aptamer[J]. Biochemical and Biophysical Research Communications, 2012, 417(1): 414-420.
[26] HAN SR, LEE SW. In vitro selection of RNA aptamer specific to Staphylococcus aureus[J]. Annals of Microbiology, 2014, 64(2): 883-885.
[27] DUAN Nuo, WU Shi-jia, CHEN Xiu-juan, et al. Selection and identification of a DNA aptamer targeted to Vibrio parahaemolyticus[J]. J. Agr. Food Chem., 2012, 60: 4 034-4 038.
[28] DUAN Nuo, DING Xiao-ying, HE liang-xing, et al. Selection, identification and application of a DNA aptamer against Listeria monocytogenes[J]. Food Control, 2013, 33(1): 239-243.
[29] 韩晓晓. 阪崎肠杆菌适配体制备及其应用[D]. 无锡: 江南大学, 2013: 17.
[30] WU Yuan-gen, ZHAN Shen-shan, WANG Lu-mei, et al. Selection of a DNA aptamer for cadmium detection based on cationic polymer mediated aggregation of gold nanoparticles[J]. Aanlyst, 2014, 139(6): 1 550-1 561.
[31] CHEN Zheng-bo, CHEN Liang, MA He, et al. Aptamer biosensor for label-free impedance spectroscopy detection of potassiumion based on DNA G-quadruplex conformation[J]. Biosensors and Bioelectronics, 2013, 48: 108-112.
[32] SVEN Klussmann. 核酸适配体手册: 功能性寡核苷酸及其应用[M]. 屈锋, 译. 北京: 化学工业出版社, 2013: 84-99.
[33] 陈秀娟. 镰刀菌毒素核酸适配体的筛选及分析应用研究[D]. 无锡: 江南大学, 2015: 44,60.
[34] NUTIU R, LI Ying-fu. In vitro selection of structure-switching signaling aptamers[J]. Angewandte Chemie-International Edition,2005,44(7):1 061-1 065.
[35] 刘晓静. SELEX技术筛选人TGF-b RⅡ亲和核酸库方法的建立[D]. 重庆: 第三军医大学, 2004: 12-19.
[36] MENDONSA S D, BOWSER M T. In vitro evolution of functional DNA using capillary electrophoresis[J]. Journal of the American Chemical Society, 2004, 126(1): 20-21.
[37] GU Hua-jie, DUAN Nuo, WU Shi-jia, et al. Graphene oxide-assisted non-immobilized SELEX of okdaic acid aptamer and the analytical application of aptasensor[J]. Scientific Reports, 2016, 6: doi:10.1038/srep21665.
[38] BENNER S A. Understanding nucleic acids using synthetic chemistry[J].Accounts of Chemical Research, 2004, 37(10): 784-797.
[39] SEFAH K, YANG Zun-yi, BRADLEY K M, et al. In vitro selection with artificial expanded genetic information systems[J]. PNAS, 2014, 111(4): 1 449-1 454.
[40] JOSHI R, JANAGAMA H, DWIVEDI HP, et al. Selection, characterization, and application of DNA aptamers for the capture and detection of Salmonella enterica serovars[J]. Molecular and Cellular Probes, 2009, 23(1): 20-28.
[41] DUAN Nuo, WU Shi-jia, CHEN Xiu-juan, et al. Selection and characterization of aptamers against Salmonella typhimurium using whole-bacterium systemic evolution of ligands by exponential enrichment(SELEX) [J]. Jouranl of Agricultural and Food Chemistry, 2013, 61: 3 229-3 234.
[42] DUAN Nuo, YAN Yi-ling, WU Shi-jia, et al. Vibrio parahaemolyticus detection aptasensor using surface-enhanced Raman scattring[J]. Food Control, 2016, 63: 122-127.
[43] CRUZ-AGUADO JA, PENNER G. Determination of Ochratoxin A with a DNA aptamer[J]. Journal of Agricultural and Food Chemistry, 2008, 56(22): 10 456-10 461.
[44] 段诺, 吴世嘉, 王周平. 核酸适配体识别荧光法检测赭曲霉毒素A[J]. 分析化学, 2011, 39(3): 300-304.
[45] PARK J H, BYUN J Y, MUN H, et al. A regeneratable, label-free, localized surface Plasmon resonance (LSPR) aptasensor for the detection of ochratoxin A[J]. Biosensors & Bioelectronics, 2014, 59: 321-327.
[46] HUANG Yu-kun, CHEN Xiu-juan, XIA Yu, et al. Selection, identification and application of a DNA aptamer against staphylococcus aureus enterotoxin A[J]. Analytical Methods, 2014, 6(3): 690-697.
[47] HUANG Yu-kun, CHEN Xiu-juan, DUAN Nuo, et al. Selection and characterization of DNA aptamers against staphylococcus aureus enterotoxin C1[J]. Food Chemistry, 2015, 166: 623-629.
[48] BRUNO J G, KIEL J L. Use of magnetic beads in selection and detection of biotoxin aptamers by electrochemiluminescence and enzymatic methods[J]. Biotechniques, 2002, 32(1): 178-180,182-183.
[49] HAES A J, GIORDANO B C, COLLINS G E. Aptamer-based detection and quantitative analysis of ricin using affinity probe capillary electrophoresis[J]. Analytical Chemistry, 2006, 78(11): 3 758-3 764.
[50] MIYAKE Y, TOGASHI H, TASHIRO M, et al. MercuryⅡ-mediated formation of thymine-HgⅡ-Thymine base paris in DNA duplexes[J]. Journal of the American Chemical Society, 2006, 128(7): 2 172-2 173.
[51] TANAKA Y, ODA S, YAMAGUCHI H, et al. 15N-15N J-coupling across HgⅡ: Direct observation of HgⅡ-Medeated T-T base pairs in a DNA duplex[J]. Journal of the American Chemical Society, 2006, 129(2): 244-245.
[52] WANG Yong, YANG Fan, YANG Xiu-rong. Colorimetric biosensing of mercuryⅡ ion using unmodified gold nanoparticle probes and thrombin-binding aptamer[J]. Biosensors & Bioelectronics, 2010, 25(8): 1 994-1 998.
[53] 凌绍明, 范燕燕, 蒋治良, 等. 核酸适配体修饰纳米金共振散射光谱探针快速检测痕量Pb2+[J]. 化学学报, 2010, 68(4): 339-344.
[54] KIM M, UM H J, BANG S, et al. Arsenic removal from Vietnamese groundwater using the arsenic-binding DNA aptamer[J]. Environmental Science & Technology, 2009, 43(24): 9 335-9 340.
[55] WU Shi-jia, DUAN Nuo, SHI Zhao, et al. Dual fluorescence resonance energy transfer assay between tunable upconversion nanoparticles and controlled gold nanoparticles for the simultaneous detection of Pb2+ and Hg2+[J]. Chemistry Aanlytical, 2014, 128: 327-336.
[56] 廖且根, 李伟红, 张金艳, 等. 基于核酸适配体与银纳米粒子探针共振光散射技术检测卡那霉素[J]. 农产品质量与安全, 2012(S1): 17-19.
[57] YADAV S K, AGRAWAL B, CHANDRA P, et al. In vitro chloramphenicol detection in a Haemophilus influenza model using an aptamer-polymer based electrochemical biosensor[J]. Biosensors & Bioelectronics, 2014, 55: 337-342.
[58] WANG Li, LIU Xian-jin, ZHANG Qiang, et al. Selection of DNA aptamers that bind to four organophosphorus pesticides[J]. Biotechnology Letters, 2012, 34(5): 869-874.

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.