Abstract
For the purpose of joint production of hydrolysis of porcine blood and microbial ecology preparation of Clostridium butyricum, it was studied that the solid medium formulation suitable for the growth and reproduction of C. butyricum was made of porcine blood powder, and the fermentation products would be used in feed. The formulation of the solid medium formulation was designed and optimized by the mean of mixture design. Moreover, the mathematical regression model of three components of solid medium was established, and the various components and the interaction between them were also discussed. Furthermore, the results showed that the optimum formula was constituted by the growth promoting factor 4.8%, corn flour 46.3%, porcine blood powder 46.3%. On this condition，the number of C. butyricum was expected to reach 8.18×107 CFU/g, and the content of free amino acid nitrogen increased from 0.02 g/100 g to 3.13 g/100 g during the fermentation. Consequently, variance analysis and verification test both showed that the mathematical model established by mixture design method was suitable for optimizing the solid fermentation of porcine blood powder medium for C. butyricum.
Publication Date
10-28-2016
First Page
40
Last Page
43
DOI
10.13652/j.issn.1003-5788.2016.10.009
Recommended Citation
Dongfang, QIN; Aimin, LIU; and Hao, LI
(2016)
"Design and optimization of solid fermentation of porcine blood powder medium for Clostridium butyricum,"
Food and Machinery: Vol. 32:
Iss.
10, Article 9.
DOI: 10.13652/j.issn.1003-5788.2016.10.009
Available at:
https://www.ifoodmm.cn/journal/vol32/iss10/9
References
[1] 宋会仪, 吴天星. 酪酸梭状芽孢杆菌微生态制剂的生物学功能及在饲料中的应用[J]. 饲料工业, 2006 , 27(12): 10-11.
[2] KUROIWA T, IWANAGA M, KOBARI K. Preventive effect of Clostridium butyricum M588 against the proliferation of Clostridium difficile during antimicrobial therapy[J]. The Journal of the Japanese Association for Infectious Diseases, 1990, 64: 1 425-1 432.
[3] KANAI T, MIKAMI Y, HAYASHI A. A breakthrough in probiotics: Clostridium butyricum regulates gut homeostasis and anti-inflammatory response in inflammatory bowel disease[J]. Journal of Gastroenterology, 2015, 50(9): 1-12.
[4] LIU Ting-ting, ZHANG Shuai, DENG Fei-yue. Effects of glutamine and Clostridium butyricum on growth performance, immune function, small intestinal morphology and microflora in weanling piglets[J]. Chinese Journal of Animal Nutrition, 2011, 23(6): 998-1 005.
[5] 赵熙, 冉陆, 杨宝兰, 等. 丁酸梭菌活菌制剂对肠道菌群影响的研究[J]. 中国微生态学杂志, 1999, 11(6): 332-333.
[6] MASAHIDE S, MANO H, TAKASHI Y, et al. Clostridium butyricum MIYAIRI 588 shows antitumor effects by enhancing the release of TRAIL from neutrophils through MMP-8[J]. International Journal of Oncology, 2013, 42(3): 903-911.
[7] 唐宝英, 朱晓慧, 刘佳. 新一代微生态制剂-酪酸梭状芽孢杆菌的研究和开发前景[J]. 中国微生态学杂志, 2000, 12(5): 297.
[8] 张晓阳, 卢忆, 马艳莉, 等. 丁酸梭菌生理功能及应用研究进展[J]. 中国食物与营养, 2012(12): 3-5.
[9] 王赟, 李爱彬, 陈湘来, 等. 猪血的开发和利用[J]. 食品与机械, 2012, 28(1): 272-274.
[10] 李雨露, 吕长鑫. 猪血的功能特性及开发利用[J]. 肉品卫生, 2001(12): 26-27.
[11] 万佳蓉, 马美湖, 周传云. 多菌种混合发酵猪血的研究[J]. 食品与机械, 2007, 23(1): 50-53.
[12] 周毅, 杨萍, 肖前程. 降解猪血蛋白的优良菌株筛选鉴定及产酶条件研究[J]. 食品与发酵工业, 2015(3): 58-63.
[13] 李斌, 章梁, 黄泽元. 酶解猪血制备血红素工艺的研究[J]. 食品与机械, 2009, 25(6): 153-156.
[14] 戚薇, 何玉慧, 李安东, 等. 酪酸梭状芽孢杆菌发酵培养基的优化[J]. 天津科技大学学报, 2010, 25(2): 18-21.
[15] 孔青, 陈琳, 周雯, 等. 丁酸梭菌淀粉培养基的优化研究[J]. 食品工业科技, 2009(12): 197-199.
[16] ZHANG Chun-hui, MA Yu-jiu, YANG Fang-xiao, et al. Optimization of medium composition for butyric acid production by Clostridium thermobutyricum using response surface ethodology[J]. Bioresource Technology, 2009, 100(18): 4 284-4 288.
[17] 徐向宏, 何明珠. 试验设计与Design-Expert、SPSS应用[M]. 北京: 科学技术出版, 2010: 161-167.
[18] 周光理. 食品分析[M]. 北京: 化学工业出版社, 2015: 101.
[19] 岳鹍, 王芃, 孙勇民. 混料设计优化复合酶酶解制备低聚木糖工艺[J]. 食品与机械, 2011, 27(6): 254-256.
[20] 阎宝清, 夏立秋, 龙如花, 等. 利用混料设计优化多杀菌素发酵培养基[J]. 食品与机械, 2014, 30(4): 200-203.