Abstract
Bee pollen soluble dietary fiber was extracted by enzymatic extraction, and the physicochemical properties was analyzed, while, the products were compared with the bee pollen soluble dietary fiber by acid extraction and alkali extraction. The optimal enzymatic extraction conditions were determined as follows: extraction temperature 50 ℃, extraction time 2 h, enzyme 2.5%, pH 4. Under the conditions, the yield of bee pollen soluble dietary fiber was 4.11%. The product is milk-white powder, and has good smell. The swelling capacity, water holding capacity and the water-solubility of the fiber were 2.03 mL/g, 1.01 g/g, 91.26%, respectively. The bee pollen soluble dietary fiber was composed of 7 kinds of monosaccharide, such as mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose and arabinose, and has the absorption peak of polysaccharides. Compared with alkali extraction and acid extraction, The bee pollen soluble dietary fiber has better water solubility and sensory quality, and is easier to apply enzymatic extraction to the beverage products development.
Publication Date
12-28-2016
First Page
184
Last Page
188
DOI
10.13652/j.issn.1003-5788.2016.12.040
Recommended Citation
Hui, ZHENG; Xiping, CHEN; Zhendan, YOU; Dongxue, XIA; and Xi, HU
(2016)
"Enzymatic extraction of bee pollen soluble dietary fiber and its physicochemical analysis,"
Food and Machinery: Vol. 32:
Iss.
12, Article 40.
DOI: 10.13652/j.issn.1003-5788.2016.12.040
Available at:
https://www.ifoodmm.cn/journal/vol32/iss12/40
References
[1] THORP R W. The collection of pollen by bees[J]. Plant Systematics and Evolution, 2000, 222: 211-223.
[2] ALMEIDA-MURADIAN L B, PAMPLONA L C, COIMBRA S, et al. Chemical composition and botanical evaluation of dried bee pollen pellets[J]. Journal of Food Composition and Analysis, 2005, 18: 105-111.
[3] YANG Kai, WU Dan, YE Xing-qian, et al. Characterization of chemical composition of bee pollen in China[J]. J. Agric. Food Chem., 2013, 61: 708-718.
[4] 杨晓萍, 吴谋成. 油菜蜂花粉多糖抗肿瘤作用的研究[J]. 营养学报, 2006, 28(2): 160-162, 166.
[5] 彭定利, 孙丽萍, 庞杰, 等. 油菜蜂花粉保肝活性组分的分离及细胞学筛选[J]. 中国食品学报, 2014, 14(3): 100-105.
[6] 刘建涛, 赵利, 苏伟, 等. 蜂花粉生物活性物质的研究进展[J]. 食品科学, 2006, 27(12): 909-912.
[7] 胡元强. 2015年蜂产品市场回顾与2016年蜂产品市场预测[J]. 蜜蜂杂志, 2016(5): 40-43.
[8] 王凯, 任向楠, 董捷, 等. 多指标评价果胶酶对油菜蜂花粉的破壁作用[J]. 食品与机械, 2015, 31(6): 164-168.
[9] XU Xiang, SUN Li-ping, DONG Jie, et al. Breaking the cells of rape bee pollen and consecutive extraction of functional oil with supercritical carbon dioxide[J]. Innovative Food Science and Emerging Technologies, 2009(10): 42-46.
[10] GAMPOS M G, WEBBY R F, MARKHAM K R, et al. Age-induced diminution of free radical scavenging capacity in bee pollens and the contribution of constituent flavonoids[J]. J. Agric. Food Chem., 2003, 51: 742-745.
[11] 陈莲君, 徐德平. 油菜蜂花粉中黄酮的分离纯化及结构鉴定[J]. 食品与机械, 2012, 28(3): 119-121.
[12] HANNAN J M A, ROKEYA B, FARUQUE O, et al. Mainous III： Effect of soluble dietary fibre fraction of Trigonella foenum graecum on glycemic, insulinemic, lipidemic and platelet aggregation status of Type 2 diabetic model rats[J]. Journal of Ethnopharmacology, 2003, 88: 73-77.
[13] GUO Wei-wei, BETA Trust. Phenolic acid composition and antioxidant potential of insoluble and soluble dietary fibre extracts derived from select whole-grain cereals[J]. Food Research International, 2013, 51: 518-525.
[14] 任志远, 李鹏飞. 麦麸可溶性膳食纤维提取及在乳品中的应用[J]. 山西农业大学学报: 自然科学版, 2010, 30(4): 305-308.
[15] ZHANG Juan, WANG Zheng-wu. Soluble dietary fiber from Canna edulis Ker by-product and its physicochemical properties[J]. Carbohydrate Polymers, 2013(92): 289-296.
[16] 郑慧, 陈希平, 常新利, 等. 响应面优化蜂花粉可溶性膳食纤维提取工艺[J]. 湖南中医药大学学报, 2015, 35(2): 20-24.
[17] 郑慧, 陈希平, 胡熙, 等. 蜂花粉可溶性膳食纤维碱提工艺[J]. 食品科技, 2015(9): 222-226.
[18] 贾冬英, 朗黄英, 姚开, 等. 柚中果皮水不溶性膳食纤维对胆固醇的吸附研究[J]. 四川大学学报: 工程科学版, 2008, 40(3): 86-90.
[19] 王庆玲, 董娟, 汪继亮. 豆渣中可溶性膳食纤维提取工艺的研究[J]. 中国酿造, 2011(3): 135-138.
[20] 伍善广, 冯学珍, 陈颖, 等. 高效液相色谱法测定金钱菇多糖的单糖组成[J]. 广西植物, 2014, 34(2): 179-182.
[21] 陈小举, 吴学凤, 姜绍通, 等. 响应面法优化半纤维素酶提取梨渣中可溶性膳食纤维工艺[J]. 食品科学, 2015, 36(6): 18-23.
[22] 王亚敏, 汪洪捷, 张卓勇. 松花粉的红外光谱、扫描电镜和X射线能谱仪分析[J]. 光谱学与光谱分析, 2005, 25(11): 1 797-1 800.
[23] 王多加, 周向阳, 金同铭, 等. 近红外光谱检测技术在农业和食品分析上的应用[J]. 光谱学与光谱分析, 2004, 24(4): 447-450.