Abstract
The optimized preparation conditions of the concentration of IgG in yak blood after salting out of saturated ammonium sulfate by single factor experiments, including the selection of the hydrolysis time, pH, temperature and the amount of enzyme, as well as the Central Composite experiment, was investigated in this study. The activity of IgG was detected by ELISA and analyzed by using the Response Surface analysis (RSA). The results showed that the optimal concentration of IgG was 27.13 mg/mL when hydrolyzed for at 39 ℃ for 2 h, and enzymolyzed at pH 4.4,
Publication Date
12-28-2016
First Page
35
Last Page
39
DOI
10.13652/j.issn.1003-5788.2016.12.008
Recommended Citation
Xueli, XIAO; Zhen, ZHANG; Shenggui, ZHANG; Rongrong, XU; Rui, DI; Jindi, LV; and Ya, LIU
(2016)
"The preparation of IgG combining the method of Alpha galactose glucoside enzyme with saturated ammonium sulfate and its activity research,"
Food and Machinery: Vol. 32:
Iss.
12, Article 8.
DOI: 10.13652/j.issn.1003-5788.2016.12.008
Available at:
https://www.ifoodmm.cn/journal/vol32/iss12/8
References
[1] 于海燕, 罗永康. 牦牛血理化特性的研究[J]. 肉类研究, 2007(6): 33-36.
[2] 朱森阳, 汪国和, 王志鹏. 畜禽血液资源的开发利用进展[J]. 养殖与饲料, 2007(1): 67-69.
[3] SASAKI M, DAVIS C L, LARSON B L. Production and turnover of IgG1 and IgG2 immunoglobulins in the bovine around parturition.[J]. Journal of Dairy Science, 1977, 59(12): 2 046-2 055.
[4] 照日格图, 敖长金, 斯日古楞. 羊IgG粗品提取方法的研究[J]. 中国饲料, 2009(12):26-27.
[5] 田明玉. 双水相萃取白蛋白和酶的初步研究[D]. 大连: 大连理工大学, 2009: 5-8.
[6] 杜雨. 超声波辅助盐析法提取及双水相法提取纯化牦牛血IgG的工艺条件研究[D]. 兰州: 甘肃农业大学, 2015: 6-8.
[7] 章晓联. 糖基化与免疫[J]. 生命科学, 2011(7): 685-694.
[8] 李孝辉, 陈声明. 微生物源α-半乳糖苷酶的研究进展[J]. 微生物学通报, 2002(2): 71-75.
[9] 任杰, 胡志和, 吴子健, 等. 超高压处理对牛初乳中IgG活性的影响[J]. 食品工业科技, 2013, 34(20): 280-283.
[10] 陈仁芳, 邢益平, 林艳, 等. 乙型肝炎病毒表面抗原中蛋白去糖基化对体液免疫应答的影响[J]. 南京医科大学学报: 自然科学版, 2007, 27(11): 1 209-1 212.
[11] 岳喜庆, 冯巧萍, 张和平, 等. 牛血清免疫球蛋白的盐析法提取与纯化[J]. 中国乳品工业, 2005(3): 16-21.
[12] 金晶. 牦牛血IgG分离纯化工艺研究[D]. 兰州: 甘肃农业大学, 2010.
[13] 刘红霞, 苏卫, 张连峰. α-半乳糖苷酶研究进展[J]. 中国比较医学杂志, 2006, 16(11): 697-720.
[14] 郭斌. 牛初乳免疫球蛋白G的规模化提取关键技术及活性保持技术的研究[D]. 合肥: 安徽农业大学, 2011: 18-19.
[15] 罗磊, 武涛, 丁霄霖. 猪血G型免疫球蛋白稳定性研究[J]. 食品与机械, 2006, 22(6): 20-22.
[16] 庞晨. 牦牛血IgG分离纯化工艺及其稳定性研究[D]. 兰州: 甘肃农业大学, 2011: 32-34.