Abstract
Cereal protein has been extremely difficult to purify and characterize owing to their heterogeneity, poor solubility and tendency to polymerize. In order to have a full understanding of structure and function in cereal protein, to realize the separation and purification of grain protein, the composition and characteristics of cereal proteins were introduced briefly, introduced the research progress comprehensive recitation on principle and model of the grain protein purification domestic and overseas in recent years. The future research prospects of cereal proteins were put forward as well.
Publication Date
2-28-2016
First Page
178
Last Page
182
DOI
10.13652/j.issn.1003-5788.2016.02.043
Recommended Citation
Wenjuan, TANG; Hongqing, ZHAO; Zhou, XU; Li, WEN; and Yunhui, CHENG
(2016)
"Advances of isolation and purification methods in cereal protein,"
Food and Machinery: Vol. 32:
Iss.
2, Article 43.
DOI: 10.13652/j.issn.1003-5788.2016.02.043
Available at:
https://www.ifoodmm.cn/journal/vol32/iss2/43
References
[1] 何国卿, 李庆龙, 李江其, 等. 全谷物杂粮米及其发展前景初探[J]. 农业机械, 2012(6): 26-29.
[2] 邱伟芬, 罗佩竹, 方勇, 等. 大米源硒代多肽的酶法制备及其抗氧化活性的研究[J]. 中国粮油学报, 2013(4): 1-7.
[3] Castro-Rubio A, García M C, Marina M L. Rapid separation of soybean and cereal (wheat, corn, and rice) proteins in complex mixtures: Application to the selective determination of the soybean protein content in commercial cereal-based products[J]. Analytica Chimica Acta, 2006, 558(1): 28-34.
[4] Schmitt C, Bovay C, Rouvet M, et al. Whey protein soluble aggregates from heating with NaCl: physicochemical, interfacial, and foaming properties[J]. Langmuir, 2007(23): 4 155-4 166.
[5] Wang Xian-sheng, Tang Chuan-he, Li Bian-sheng, et al. Effects of high-pressure treatment on some physicochemical and functional properties of soy protein isolates[J]. Food Hydrocolloids, 2008(22): 560-567.
[6] 梁彬霞, 朱豪. 谷物蛋白的结构功能及开发特性的研究进展[J]. 农产品加工·学刊(下), 2013(6): 63-65.
[7] Yu Zi-tong, Han Cai-xia, Wang Shu, et al. Fast separation and characterization of water-soluble proteins in wheat grains by reversed-phase ultra performance liquid chromatography (RP-UPLC)[J]. Journal of Cereal Science, 2013, 57(3): 288-294.
[8] 王章存, 康艳玲. 国内外谷物蛋白发展概况[J]. 中国食品添加剂, 2006(5): 110-113.
[9] Amano M, Ogawa H, Kojima K, et al. Identification of the major allergens in wheat flour responsible for baker's asthma[J]. Biochemistry Journal, 1998, 330(3): 1 229-1 234.
[10] 杜金娟, 吴琼英, 贾俊强. 甜高粱蛋白的氨基酸组成和营养学评价[J]. 食品工业, 2013(7): 143-146.
[11] 杜传来, 王佳红, 周娴, 等.可食性膜及其在果蔬保鲜中的应用[J]. 包装与食品机械, 2004, 22(1): 46-49.
[12] 林峰, 蔡木易, 易维学. 玉米蛋白深加工现状及发展趋势[J]. 食品与发酵工业, 2007, 32(11): 123-127.
[13] 赵文扬, 康明丽, 王蕾娜, 等. 玉米蛋白粉的综合利用[J]. 食品工程, 2014(2): 3-5.
[14] 刘建垒, 张玲, 郝利平. 燕麦蛋白提取分离的研究进展[J]. 食品研究与开发, 2014(1): 132-136.
[15] 张淑红, 冯硕, 李正平, 等. 高效液相色谱(HPLC)分离和检测蛋白质[J]. 河北农业大学学报, 2003(z1): 148-151.
[16] 赵龙刚, 宋希云, 史雪辉, 等. 小麦胚乳醇溶蛋白反相高效液相色谱(RP—HPLC)分离方法的建立[J]. 现代仪器, 2006(6): 78-80.
[17] 晏月明, 刘广田. 毛细管区带电泳分离小麦种子醇溶蛋白的研究[J]. 色谱, 1998(3): 68-70.
[18] Naeem H A, Sapirstein H D. Ultra-fast separation of wheat glutenin subunits by reversed-phase HPLC using a superficially porous silica-based column[J]. J. Cereal Sci., 2007(46): 157-168.
[19] Gao Li-yan, Ma Wu-jun, Chen Jing, et al. Characterization and comparative analysis of wheat high molecular weight glutenin subunits by SDS—PAGE, RP—HPLC, HPCE, and MALDI—TOF—MS[J]. Journal of Agricultural and Food Chemistry, 2010, 58(5): 2 777-2 786.
[20] Schober T J, Bean S R, Kuhn M. Gluten proteins from spelt (Triticum aestivum ssp. Spelta cultivars: A rheological and size-exclusion high-performance liquid chromatography study[J]. Journal of Cereal Science, 2006, 44(2): 161-173.
[21] Morel M H, Dehlon P, Autran J C, et al. Effects of temperature, sonication time, and power settings on size distribution and extractability of total wheat flour proteins as determined by size-exclusion high-performance liquid chromatography[J]. Cereal Chemistry, 2000, 77(5): 685-691.
[22] 张锐昌, 王绮, 张应龙, 等. Tricine—SDS—PAGE测定小麦蛋白酶解物分子量分布[J]. 食品研究与开发, 2012, 33(12): 168-171.
[23] Frederick F, Kim D. Preparation and characterization of rice protein isolations[J]. Journal of the American Oil Chemists' Society, 2000, 77(8): 268-272.
[24] 葛娜, 易翠平, 姚惠源. 碱性蛋白酶提取大米水解蛋白的研究[J]. 粮食与饲料工业, 2006(4): 25-27.
[25] Oszvald M, Tmskzi S, Larroque O, et al. Characterization of rice storage proteins by SE—HPLC and micro z-arm mixer[J]. Journal of Cereal Science, 2008, 48(1): 68-76.
[26] 方勇, 杨文建, 马宁, 等. 体积排阻色谱—电感耦合等离子体质谱分析富硒大米含硒蛋白组成[J]. 分析化学, 2013, 41(6): 882-887.
[27] 刘珊珊, 陈季旺, 陈露, 等. 大米镉结合蛋白的凝胶层析纯化及生化性质[J]. 食品安全质量检测学报, 2015(6): 2 269-2 274.
[28] Blackwell D L, Bean S R. Separation of alcohol soluble sorghum proteins using non-porous cation-exchange columns[J]. Journal of Chromatography A, 2012, 230(1): 48-53.
[29] Bean S R, Ioerger B P, Blackwell D L. Separation of kafirins on surface porous reversed-phase high-performance liquid chromatography columns[J]. Journal of Agricultural and Food Chemistry, 2010, 59(1): 85-91.
[30] Celus I, Brijs K, Delcour J A. The effects of malting and mashing on barley protein extractability[J]. Journal of Cereal Science, 2006, 44(2): 203-211.
[31] 刘宝祥, 朴永哲, 苏政波, 等. 不同国产酿造大麦蛋白质双向电泳差异性分析[J]. 作物杂志, 2013(6): 23-26.
[32] Rodriquez-Nogales J M, Garcia M C, Marina M L. Development of a perfusion reversed-phase high performance liquid chromatography method for the characterization of maize products using multivariate analysis[J]. J. Chromatogr. A, 2006, 104(1): 91-99.
[33] Rodriquez-Nogales J M, Garcia M C, Marina M L. Monolithic supports for the characterization of commercial maize products based on their chromatographic profile. Application of experimental design and classification techniques[J]. J. Agric. Food Chem., 2006(54): 1 173-1 179.
[34] Nieto-Nieto T V, Wang Yi-xiang, Ozimek L, et al. Effects of partial hydrolysis on structure and gelling properties of oat globular proteins[J]. Food Research International, 2014(55): 418-425.
[35] 张晓平, 董银卯, 刘永国, 等. 中空纤维膜分离燕麦蛋白工艺及膜清洗方案[J]. 农业工程学报, 2010, 3(3): 332-340.
[36] Klose C, Schehl B D, Arendt E K. Fundamental study on protein changes taking place during malting of oats[J]. Journal of Cereal Science, 2009, 49(1): 83-91.
[37] 单树花, 武海丽, 李宗伟, 等. 小米米糠中抗癌细胞增殖活性蛋白的分离纯化[J]. 食品科学, 2013, 34(9): 296-300.
[38] He Xuan-hui, Liu Hong-zhi, Liu Li, et al. Effects of high pressure on the physicochemical and functional properties of peanut protein isolates[J]. Food Hydrocolloids, 2014(36): 123-129.
[39] Bean S R, Bietz J A, Lookhart G L. High-performance capillary electrophoresis of cereal proteins[J]. Journal of Chromatography A, 1998, 814(1): 25-41.
[40] Castro-Rubio A, García M C, Marina M L. Rapid separation of soybean and cereal (wheat, corn, and rice) proteins in complex mixtures: Application to the selective determination of the soybean protein content in commercial cereal-based products[J]. Analytica Chimica Acta, 2006, 558(1): 28-34.
[41] Bietz J A. Separation of cereal proteins by reversed-phase high-performance liquid chromatography[J]. Journal of Chromatography A, 1983(255): 219-238.
[42] Bean S R, Lookhart G L. Separation of wheat proteins by two-dimensional reversed-phase high-performance liquid chromatography plus free zone capillary electrophoresis 1[J]. Cereal Chemistry, 1997(74): 758-765.