Abstract
In order to study the ultra-high pressure extraction of cordycepin from Cordyceps militaris fresh juice, the effect of opration conditions such as pressure, time, solid-liquid ratio and cycle number were analyzed. On the basis of single-factor test, the optimum extraction technology of cordycepin and mathematical regression model were established by response surface methodology. The result showed that the optimal extraction conditions were as followed: extraction pressure of 418 MPa, extraction time of 33 min, solid-liquid ratio of 1∶2 (m∶V), cycle number of 1. Under these conditions, the measured value of dissolved quantity of cordycepin was 84.69 mg/g of extract, to which the relative standard deviation of predicted value (84.03 mg/g of extract) was 0.61%. The standardized regression equation can be used to predict the extraction yield of cordycepin from C. militaris fresh juice.
Publication Date
4-28-2016
First Page
187
Last Page
191,200
DOI
10.13652/j.issn.1003-5788.2016.04.043
Recommended Citation
Shuangjie, ZHU; Lili, DONG; Jian, PAN; Qingfei, DU; Xia, LUO; and Weizhu, ZHANG
(2016)
"Optimizationon improving dissolved quantity of cordycepin from Cordyceps militari juice by ultra-high pressure processing,"
Food and Machinery: Vol. 32:
Iss.
4, Article 43.
DOI: 10.13652/j.issn.1003-5788.2016.04.043
Available at:
https://www.ifoodmm.cn/journal/vol32/iss4/43
References
[1] 陈斌, 贾晓斌. 鲜药物质基础的研究进展与研究策略[J]. 中草药, 2012, 43(3): 592-597.
[2] Wang Mi, Meng Xin-yu, Yang Rui-lei, et al. Cordyceps militaris polysaccharides can enhance the immunity and antioxidation activity in immunosuppressed mice[J]. Carbohydrate Polymers, 2012, 89(2): 461-466.
[3] Yu Hui-mei, Wang Bor-sen, Huang Shiow-Chyn, et al. Comparison of protective effects between cultured Cordyceps militaris and natural Cordyceps sinensis against oxidative damage[J]. Journal of Agriculture Food Chemistry, 2006, 54(8): 3 132-3 138.
[4] Won Soyoung, Park Eunhee. Anti-inflammatory and related pharmacological activities of cultured mycelia and fruiting bodies of Cordyceps militaris[J]. Journal of Ethnopharmacology, 2005, 96(2): 555-561.
[5] Zhou Xiao-xia, Luo Li-ping, Dressel Waike, et al. Cordycepin is an immunoregulatory active ingredient of Cordyceps sinensis[J]. The American Journal of Chinese Medicine, 2008, 36(5): 967-980.
[6] Lee Hongjue, Burger Petra, Vogel Marianne, et al. The nucleoside antagonist cordycepin causes DNA double strand breaks in breast cancer cells[J]. Investigational New Drugs, 2012, 30(1): 1 917-1 925.
[7] Zhu Shang-jie, Pan Jian, Liang Juan, et al. Comparisons on enhancing the immunity of fresh and dry Cordyceps militaris in vivo and in vitro [J]. Journal of Ethnopharmacology, 2013, 149(2): 713-719.
[8] 苗明三, 马霄. 中药鲜药加工方法探讨[J]. 时珍国医国药, 2009, 20(5): 1 208-1 209.
[9] 陆怡, 颜慧庚, 沈士明. 超高压生物处理技术及装备研究进展[J]. 食品与机械, 2007, 23(5): 109-111.
[10] 陈瑞战, 张守勤, 刘志强. 超高压技术在中药有效成分提取中的应用[J]. 中草药, 2007, 38(12): 1 905-1 908.
[11] 陈瑞战. 超高压提取人参皂苷工艺及机理研究[D]. 长春: 吉林大学, 2005: 5-9.
[12] 凌庆枝, 李晓, 魏兆军, 等. 桑叶多糖超高压提取工艺研究[J]. 食品与机械, 2008, 24(2): 50-52.
[13] 宋丽军, 侯旭杰, 李雅雯, 等. 核桃青皮中多酚的超高压提取工艺优化[J]. 食品与机械, 2015, 31(4): 178-182.
[14] 陈尚卫, 虞锐鹏, 朱松, 等. 高效液相色谱法同时测定蛹虫草中四种核苷类活性成分[J]. 食品与机械， 2006, 22(6): 107-109.
[15] 曾庆梅, 潘见, 谢慧明, 等. 超高压处理对辣根过氧化物酶二级结构及其活力的影响[J]. 食品科学, 2005, 26(5): 29-33.
[16] 陈井旺, 木泰华. 超高压技术对食品中酶的作用研究进展[J]. 食品研究与开发, 2010, 31(1): 178-182.
[17] 张洪鑫, 陈小泉, 蒋玲玲. 金属离子对纤维素酶内切酶和外切酶活性的影响[J]. 纤维素科学与技术, 2011, 9(4): 6-13.
[18] 翁梁, 温鲁. 不同pH值提取液浸提虫草素研究[J]. 食品科学, 2006, 27(11): 274-276.
[19] 张泽志, 韩春亮, 李成未. 响应面法在试验设计与优化中的应用[J]. 河南教育学院学报: 自然科学版, 2011, 20(4): 34-37.