•  
  •  
 

Abstract

The optimum condition of ultrasonic extraction of Polygala tenuifolia Willd. Polysaccharide (PTP) was investigated. The results showed, the optimum condition was ultrasonic extraction time 30 min, ultrasonic extraction, temperature 67 ℃, ratio of water to raw material 10∶1(mL/g), and extraction 2 times, the yield of polysaccharide was(5.87±0.21)% (n=3). IR spectra predicted PTP had typical characteristic absorption peaks which were neutral polysaccharide and free from urinic acid. In vitro antioxidant activities showed PTP had effects on DPPH radical and hydroxyl radical.The IC50 of it against DPPH radical and hydroxyl radical was 0.83 mg/mL, 1.29 mg/mL.

Publication Date

5-28-2016

First Page

152

Last Page

156,193

DOI

10.13652/j.issn.1003-5788.2016.05.036

References

[1] 国家药典委员会. 中国药典: 一部[S]. 北京: 中国医药科技出版社, 2015: 156-157.
[2] 刘大伟, 康利平, 马百平. 远志化学及药理作用研究进展[J]. 国际药学研究杂志, 2012, 39(1): 32-36, 44.
[3] Liu Jun, Stefan Willfr, Xu Chun-lin. A review of bioactive plant polysaccharides: Biological activities, functionalization, and biomedical applications[J]. Bioactive Carbohydrates and Dietary Fibre, 2015, 5(1): 31-36.
[4] 时宵丽, 姚春霞, 林晓, 等. 多糖药物应用与研究进展[J]. 中国新药杂志, 2014, 23(9): 1 057-1 062.
[5] Xin Tao, Zhang Fu-bin, Jiang Qiu-ying, et al. Purification and antitumor activity of two acidic polysaccharides from the roots of Polygala tenuifolia [J]. Carbohydrate Polymers, 2012, 90(4): 1 671-1 676.
[6] 魏永俊, 周秋菊, 黄树颖, 等. 小远志多糖的提取及纯化[J]. 江苏师范大学学报: 自然科学版, 2014, 32(1): 60-63.
[7] 方元, 许铭强, 汪欣蓓, 等. 超声波辅助提取哈密大枣多糖的工艺优化[J]. 食品与机械, 2014, 30(2): 175-180.
[8] Jing Yong-shuai, Zhu Jian-hua, Liu Ting, et al. Structural characterization and biological activities of a novel polysaccharide from cultured Cordyceps militaris and its sulfated derivative[J]. Journal of Agricultural and Food Chemistry, 2015, 63(13): 3 464-3 471.
[9] 吴兰芳, 蒋爱民, 郭善广, 等. 凝固型紫薯酸乳发酵工艺及抗氧化活性研究[J]. 食品与机械, 2013, 29(5): 198-203.
[10] Solomon Rajesh Thambiraj, Michael Phillips, Sundar Rao Koyyalamudi. Antioxidant activities and characterisation of polysaccharides isolated from the seeds of Lupinus angustifolius[J]. Industrial Crops and Products, 2015, 74(11): 950-856.
[11] Jing Yong-shuai, Huang Li-jiao, Lv Wen-jie, et al. Structure characterization of a novel polysaccharide from pulp tissues of Litchi chinensis and its immnunomodulatory activity[J]. Journal of Agricultural and Food Chemistry, 2014, 62(4): 902-911.

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.