Preperation of feruloylated saccharides from corn straw by enzymatic hydrolysis

Authors

YOU Mengzhu, The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, China; National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, Jiangsu 214122
LU Jian, The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, China; National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, Jiangsu 214122
CAI Guolin, The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, China; National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, Jiangsu 214122

Abstract

Multi-stage cyclone grinding can be used to change the physical structure of corn straw, which can be degraded easier by enzymes to release feruloylated saccharides. By the same enzymatic hydrolysis, the content of feruloylated saccharides released from multi-stage cyclone grinding corn straw increased by 3.34 fold than that without pretreatment. Commercial complex enzymes, such as Validase TRL, HSP 6000 BG, Rapidase Smart Clear, xylosidase and arabinfuranosidease were used to hydrolyze multi-stage cyclone grinding corn straw, and it showed that Validase TRL had the best effect, and the yield of feruloylated saccharides was 1.46 μmol/g, increased by 3.48 fold than that without hydrolysis. The best combination of synergy enzymatic hydrolysis was Validase TRL 100 U/g, xylosidase 50 U/g and arabinfuranosidease 50 U/g, and the highest yield of feruloylated saccharides was 2.87 μmol/g. After the hydrolysater was pured by macroreticular resin, the recovery of feruloylated saccharides reached 81.44%.

Publication Date

6-28-2016

First Page

189

Last Page

192,200

DOI

10.13652/j.issn.1003-5788.2016.06.045

Recommended Citation

Mengzhu, YOU; Jian, LU; and Guolin, CAI (2016) "Preperation of feruloylated saccharides from corn straw by enzymatic hydrolysis," Food and Machinery: Vol. 32: Iss. 6, Article 45.
DOI: 10.13652/j.issn.1003-5788.2016.06.045
Available at: https://www.ifoodmm.cn/journal/vol32/iss6/45

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