Research on biological activity of anthocyanins in wild blueberry

Authors

JING Zhixing, College of Food science & Technology, Shanghai Ocean University, Marine bio-pharmaceutical Department, Shanghai 201306, China
LV Liuzhuang, College of Food science & Technology, Shanghai Ocean University, Marine bio-pharmaceutical Department, Shanghai 201306, China
XU Jianfeng, College of Food science & Technology, Shanghai Ocean University, Marine bio-pharmaceutical Department, Shanghai 201306, China
WANG Wenhan, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China
FAN Hua, Institut für Laboratoriumsmedizin Klinische Chemie und Pathobiochemie, Campus Virchow Klinikum, Charite-Universittsmedizin, Berlin 13353, Germany

Abstract

Anthocyanins was extracted from wild blueberry. The evaluations were made on activities of antioxidant in vitro with superoxide anion and hydrogen peroxide radical scavenging, mice in vivo anti-inflammatory with mice swollen foot swollen ear inflammatory model and anti-tumor activity in vitro by AlamarBlue method after purified by XAD-7 macroporous resin. Results: the anthocyanins of Chinese wild blueberry have obvious antioxidant capacity, and have a positive correlation between the concentration; in concentration of 800 μg/mL, the superoxide anion scavenging rate was 86.86%, and at a concentration of 100 μg/mL, the hydrogen peroxide scavenging rate come to 73.93%; in addition, the anthocyanins have good anti-inflammatory capabilities, at 100 μg/g oral dose, inhibition of ear swelling was 47.04%, which foot swelling inhibition in the first four hours reach a maximum; moreover, their antitumor activity is also very significant, the inhibition of K562 leukemia cell proliferation IC₅₀ was 79.01 μg/mL. The results above show that Chinese wild blueberry has a variety of biological activity, which offer the foundation in their in-depth study of the development and utilization.

Publication Date

7-28-2016

First Page

132

Last Page

135,195

DOI

10.13652/j.issn.1003-5788.2016.07.031

Recommended Citation

Zhixing, JING; Liuzhuang, LV; Jianfeng, XU; Wenhan, WANG; and Hua, FAN (2016) "Research on biological activity of anthocyanins in wild blueberry," Food and Machinery: Vol. 32: Iss. 7, Article 31.
DOI: 10.13652/j.issn.1003-5788.2016.07.031
Available at: https://www.ifoodmm.cn/journal/vol32/iss7/31

References

[1] 高志江. 云南文山地区野生蓝莓资源分布[J]. 农村实用技术2010(4): 39-41.
[2] 姜文洁, 赵勇, 吴启华, 等. 野生蓝莓提取物对四种致病菌的抑制作用[J]. 天然产物研究与开发, 2014(26): 1 884-1 888.
[3] 魏银花, 申迎宾, 王立, 等. 紫米多酚提取工艺及抗氧化活性研究[J]. 食品与机械, 2013, 29(3): 111-115.
[4] FARAH S, BETA T. Saskatoon and wild blueberries have higher anthocyanin contents than other manitoba berries [J]. Journal of Agricultural and Food Chemistry, 2007, 55(26): 10 832-10 838.
[5] VAN D, PIVONKA E. Overview of the health benefits of fruit and vegetable consumption for the dietetics professional[J]. Journal of the American Dietetic Association, 2000(10): 1 511-1 521.
[6] WU Xian-li, ALEXANDER G S. Mitigation of Inflammation with Foods[J]. Agric. Food Chem., 2012, 60: 6 703-6 717.
[7] WU Xian-li, KANG Jie, XIE Cheng-hui, et al. Dietary blueberries attenuate atherosclerosis in apolipoprotein e-deficient mice by upregulating antioxidant enzyme expression[J]. Journal of Nutrition, 2010(7): 1 628-1 632.
[8] 吴林. 中国蓝莓35年[J]. 吉林农业大学学报, 2016, 38(1): 1-11.
[9] 史海芝, 刘惠民. 国内外蓝莓研究现状[J]. 江苏林业科技, 2009(4): 48-51.
[10] KALT W, BLUMBERG J B, MCDONALD J E, et al. Identification of anthocyanins in the liver, eye, and brain of blueberry-fed pigs[J]. Journal of Agricultural and Food Chemistry, 2008, 56(3): 705-712.
[11] PRIOR R L, WU Xian-li, GU Li-wei, et al. Purified berry anthocyanins but not whole berries normalize lipid parameters in mice fed an obesogenic high fat diet[J]. Molecular Nutrition & Food Research, 2009, 53 (11): 1 406-1 418.
[12] 郭蔼光, 王振镒. 邻苯三酚自氧化—化学发光法测定SOD活性[J]. 植物生理学通讯, 1989(3): 54-57.
[13] 王钦博, 杨焱, 刘艳芳, 等. 一年生和两年生桑黄子实体的成分和生物活性[J]. 食用菌学报, 2010, 17(2): 71-75.
[14] 吴斯琴毕力格, 包勒朝鲁, 那生桑. 沙棘药理作用研究进展[J]. 中国药业, 2015, 24(1): 95-96.
[15] 邹宇晓, 刘学铭, 廖森泰, 等. 荔枝壳花青素对大鼠佐剂性关节炎的治疗作用研究[J]. 营养学报, 2010(3): 257-260.
[16] ZIKRI N, RIEDL M, WANG Li-shu, et al. Black raspberry components inhibit proliferation, induce apoptosis and modulate gene expression in rat esophageal epithelial cells[J]. Nutrition and Cancer-an International Journal, 2009, 61(6): 816-826.
[17] BOIVIN D, BLANCHETTE M, BARRETTE S, et al. Inhibition of cancer cell proliferation and suppression of TNF-induced activation of NF kappa B by edible berry juice[J]. Anticancer Research, 2007, 27(2): 937-948.