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Abstract

The response surface methodology was used to optimize microwave-assisted extraction process of polysaccharides from walnut husks. Compared with the ascorbic acid, the preliminary antioxidant activities of the polysaccharides was evaluated by investigated the ·OH, DPPH·, ABTS+· free radical scavenging capacity and total antioxidant capacity. The results showed that the optimal conditions were water-feed ratio 25∶1(mL/g), microwave power 750 W, extraction time 8.5 min, extract 2 times. Under these conditions, the yield of polysaccharides was 10.17%. The results of anti-oxidation experiment showed that the walnut husks polysaccharides had some scavenging effects, but not as good as ascorbic acid. The walnut husks polysaccharides scavenged the ·OH, DPPH·, ABTS+· free radical as IC50 values of 83 975.520, 628.800 and 694.733 μg/mL, and the FRAP(total antioxidant capacity) were 120.42 μmol/mL.

Publication Date

7-28-2016

First Page

146

Last Page

151

DOI

10.13652/j.issn.1003-5788.2016.07.034

References

[1] KESKIN D, CEYHAN N, UGUR A. Chemical composition and in vitro antimicrobial activity of walnut (Juglans Regia) green husks and leaves from west anatolia[J]. Journal of Pure and Applied Microbiology, 2012,6(2): 583-588.
[2] 徐巍. 青龙衣的药用研究概述[J]. 中医药信息, 2002, 19(6): 13-14.
[3] 陆俊, 赵安琪, 成策, 等. 核桃营养成分与生理活性及开发利用[J]. 食品与机械, 2014, 30(6): 238-242.
[4] CARVALHO M, FERREIRA P J, MENDES V S, et al. Human cancer cell antiproliferative and antioxidant activities of Juglans Regia L.[J]. Food and Chemical Toxicology, 2010, 48(1): 441-447.
[5] 刘元慧. 山核桃外果皮的化学成分及生物活性研究[D]. 杭州: 浙江林学院, 2009: 10-13.
[6] 季宇彬, 陈海继, 汲晨锋. 青龙衣多糖的提取及单糖组分和质量分数测定[J]. 哈尔滨商业大学学报: 自然科学版, 2006(4): 1-4.
[7] 于翠莲. 核桃青皮粗多糖的提取、理化性质及其抗氧化活性的研究[D]. 保定: 河北农业大学, 2012.
[8] 岳春, 李靖靖, 方永远. 虫草多糖微波辅助提取工艺的优化[J]. 食品与机械, 2014, 30(1): 192-195.
[9] 孟娟. 蒽酮-硫酸比色法测定甘草残渣中多糖含量[J]. 天津化工, 2011, 25(5): 45-46.
[10] 何玲玲, 王新, 刘彬, 等. 板栗多糖的分离纯化及抗氧化活性研究[J]. 食品与机械, 2010, 26(2): 72-75.
[11] 涂宗财, 寇玉, 王辉, 等. 荷叶多糖的超声波辅助提取和抗氧化活性[J]. 食品科学, 2013, 34(16): 108-112.
[12] 廖月霞, 王笑娜, 孔桂美, 等. 半枝莲乙醇提取物体外抗氧化活性研究[J]. 时珍国医国药, 2012, 23(3): 520-522.
[13] BENZIE I F, STRAIN J J. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay[J]. Analytical Biochemistry, 1996, 239(1): 70-76.
[14] 胡园园, 方淑玲, 杨虎清, 等. 微波辅助提取山茱萸多糖工艺优化[J]. 食品与机械, 2011, 27(5): 93-95.
[15] 申伟崧, 刘毅, 陈效毅. 响应面分析法优化微波辅助提取银杏外种皮多糖的工艺研究[J]. 中国野生植物资源, 2014(5): 19-22.
[16] 陈玉霞, 刘建华, 林峰, 等. DPPH和FRAP法测定41种中草药抗氧化活性[J]. 实验室研究与探索, 2011(6): 11-14.

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