Wide-scope screening of multiple and illegal additives in foods by high performance liquid chromatography-high resolution mass spectrometry

Authors

ZHANG Fan, Changsha Environmental Protection College, Changsha, Hunan 410004, China; Hunan Academy of Science and Technology for Inspection and Quarantine, Changsha, Hunan 410004, China
YAN Hongfei, Hunan Academy of Science and Technology for Inspection and Quarantine, Changsha, Hunan 410004, China; Hunan Entry-Exit Inspection and Quarantine Bureau, Changsha, Hunan 410004, China
DAI Jieyun, Hunan Entry-Exit Inspection and Quarantine Bureau, Changsha, Hunan 410004, China
WU Bo, Hunan Entry-Exit Inspection and Quarantine Bureau, Changsha, Hunan 410004, China
HUANG Zhiqiang, Hunan Academy of Science and Technology for Inspection and Quarantine, Changsha, Hunan 410004, China; Hunan Entry-Exit Inspection and Quarantine Bureau, Changsha, Hunan 410004, China
ZHANG Ying, Hunan Academy of Science and Technology for Inspection and Quarantine, Changsha, Hunan 410004, China; Hunan Entry-Exit Inspection and Quarantine Bureau, Changsha, Hunan 410004, China
WANG Meiling, Hunan Academy of Science and Technology for Inspection and Quarantine, Changsha, Hunan 410004, China; Hunan Entry-Exit Inspection and Quarantine Bureau, Changsha, Hunan 410004, China

Abstract

A method was developed screening, confirming and quantifying multiple additives and banned dyes in wine, juice and other beverages by liquid chromatography-ion trap-time of flight tandem mass spectrometry (LC-MS-IT-TOF). The accurate and multiple-stage mass spectrometry database of 41compounds was established. The analytes in samples were diluted with water-methanol (v/v). In the chromatographic analysis, target compounds were separated on a C₁₈ column (150 mm × 4.6 mm, 5 μm) with the gradient elution using the mobile phases of methanol and 2 mM ammonium acetate. The results showed that the limits of detection were 0.005～1.0 mg/kg. The method validation was carried out at three spiking levels, and the recoveries were 41.2%～114.1%, with the relative standard deviations (RSDs) of 4.3%～16.4%. The screening of analytes was performed by precision mass matching and library searching. The retention time, isotopic abundance and multiple-stage ion mass spectral was employed in this confirmation. This method is simple, fast, credible and highly sensitive and can be applied to screening both multiple and illegal additives in foods without reference standards, reducing the analytical time and improving detection efficiency.

Publication Date

8-28-2016

First Page

42

Last Page

48,62

DOI

10.13652/j.issn.1003-5788.2016.08.011

Recommended Citation

Fan, ZHANG; Hongfei, YAN; Jieyun, DAI; Bo, WU; Zhiqiang, HUANG; Ying, ZHANG; and Meiling, WANG (2016) "Wide-scope screening of multiple and illegal additives in foods by high performance liquid chromatography-high resolution mass spectrometry," Food and Machinery: Vol. 32: Iss. 8, Article 11.
DOI: 10.13652/j.issn.1003-5788.2016.08.011
Available at: https://www.ifoodmm.cn/journal/vol32/iss8/11

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