•  
  •  
 

Abstract

A headspace-solid phase microextraction/gas chromatography-mass spectrometry (HS—SPME/GC—MS) was used to analyze the volatiles of rice contaminated with Aspergillus flavus and their ion chromatograms were obtained. Combined TurboMass and NIST Search software, 26 volatile metabolites were identified in rice contaminated with Aspergillus flavus, including five kinds of aldehydes, six kinds of alcohols, one kind of ketone, nine kinds of hydrocarbons, one kind of ester, one kind heterocyclic, one kind of organic acid, one kind of amine. Based on aflatoxin catabolism and substance transformation in the rice, 2-hydroxy inferred-propionamide and Heptaldehyde are typically volatile component group, which would be used as typical markers to identify rice contaminated by Aspergillus flavus, but also provide a theoretical basis for development of early and rapid detection techniques for identification of contamination rice.

Publication Date

8-28-2016

First Page

49

Last Page

54

DOI

10.13652/j.issn.1003-5788.2016.08.012

References

[1] 付鹏程, 李荣涛, 谢刚, 等. 稻谷真菌毒素污染调查与分析[J]. 粮食贮藏, 2004, 33(4): 49-51.
[2] 吴丹. 黄曲霉毒素在粮食和食品中的危害及防治[J]. 粮食加工, 2007, 32(3): 91-94.
[3] 王建辉, 王秀, 陈奇, 等. 亚油酸氧化分解过程中组成成分及挥发性物质的变化[J]. 食品与机械, 2016, 32(5): 5-10.
[4] 潘丽红. 固相微萃取技术及其应用[J]. 金陵科技学院学报, 2013, 29(2): 41-45.
[5] 兰欣, 汪东风, 张莉, 等. HS—SPME法结合GC—MS分析崂山绿茶的香气成分[J]. 食品与机械, 2012, 28(5): 96-101.
[6] 周显青, 张玉荣, 赵秋红, 等. 稻谷新陈度的研究(四): 稻谷贮藏过程中挥发性物质的变化及其与新陈度的关系[J]. 粮食与饲料工业, 2005(2): 1-3.
[7] 宋伟, 张明, 张婷筠. 基于GC/MS 的贮藏粳稻谷挥发物质变化研究[J]. 中国粮油学报, 2013, 28(11): 97-102.
[8] 凌家煜, 夏奇志, 蒋美英. 粮食中挥发性羰基化合物(VVC)的组成分析与含量测定[J]. 粮食贮藏, 1988, 17(1): 20-25.
[9] 刘敬科, 郑理, 赵思明, 等. 蒸煮方法对米饭挥发性成分的影响[J]. 中国粮油学报, 2007, 22(5): 12-15.
[10] Sirisoontaralak P, Noomhorm A. Changes to physicochemical properties and aroma of irradiated rice[J]. Journal of Stored Products Research, 2006, 42(3): 264-276.
[11] Bryant R J, McClung A M. Volatile profiles of aromatic and non-aromatic rice cultivars using SPME/GC—MS[J]. Food Chemistry, 2011, 124(2): 501-513.
[12] 刘源, 周光宏, 徐幸莲. 固相微萃取及其在食品分析中的应用[J]. 食品与发酵工业, 2003, 29(7): 83-87.
[13] 王镜岩. 生物化学[M]. 5版. 北京: 高等教育出版社, 2002.

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.