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Abstract

In order to evaluate the content of polyphenol and flavonoid, as well as antioxidant activity in different parts of Akebia trifoliata., the ultrasonic-assisted and reflux method were used. The content of polyphenol and flavonoids, and antioxidant capacity in different parts were compared. The results showed that the ultrasonic extraction method was more efficient than the reflux extraction method, among the all extracts, the highest content of polyphenol and flavonoid, and strongest antioxidant capacity was found in cane extract by ultrasonic-assited (CSJ). The contents of the total polyphenol, flavonoid in CSJ as (63.033±0.482) mg/g, (26.395±0.731) mg/g, respectively, and the DPPH, ABTS, FRAP antioxidant activity value in CSJ as (380.860±5.462) μmol Trolox/g, (337.181±1.586) μmol Trolox/g and (387.406±1.773) μmol Trolox/g, respectively. The polyphenol, flavonoids content and antioxidant activity for all extracts were showing the following tendency: cane > leaf > peel > seed > sarcocarp. Spearman correlation study shows that the content of polyphenol, flavonoids and strongly related to the antioxidant activity. The research results provide experimental basis for deep processing of Akebia Trifoliata..

Publication Date

8-28-2016

First Page

132

Last Page

135,223

DOI

10.13652/j.issn.1003-5788.2016.08.032

References

[1] 班小重, 万明长, 张朝君, 等. 野生果树八月瓜的资源收集与利用评价[J]. 贵州农业科学, 2008, 36(4): 17-19.
[2] 张晓蓉, 杨朝霞, 刘世彪, 等. 湘西地区木通果实微量元素的测定[J]. 中国野生植物资源, 2003, 22(1): 44-46.
[3] 万明长, 刘学武, 班小重, 等. 三叶木通栽培条件下果实性状及营养成分分析[J]. 贵州农业科学, 2008, 36(3): 121-122.
[4] 谢小霞, 葛发欢. 三叶木通药学研究现状与展望[J]. 现代医药卫生, 2009, 25(14): 2 165-2 167.
[5] 吴永朋, 原雅玲, 肖娅萍. 三叶木通的研究进展[J]. 陕西林业科技, 2013(1): 31-34.
[6] MIMAKI Y, DOI S, KURODA M, et al. Triterpene glycosides from the stems of Akebia quinata[J]. Chemical & Pharmaceutical Bulletin, 2007, 55(9): 1 319-1 324.
[7] MIMAKI Y, KURODA M, YOKOSUKA A, et al. Triterpenes and Triterpene Saponins from the Stems of Akebia trifoliata[J]. Chemical & Pharmaceutical Bulletin, 2004, 35(3): 960-965.
[8] JIANG Dan, SHI She-po, CAO Ji-juan, et al. Triterpene saponins from the fruits of Akebia quinata[J]. Biochemical Systematics & Ecology, 2008, 36(2): 138-141.
[9] 关树光, 於文博, 潘文君, 等. 三叶木通中酚醇和酚醇苷的生物活性研究[J]. 中国民族民间医药, 2015(23): 12-13.
[10] 张铮, 冯航, 王喆之. 三叶木通藤茎中三萜皂苷类成分积累动态的研究[J]. 中药材, 2014, 37(7): 1 130-1 133.
[11] 邵显会, 肖娅萍, 张珂, 等. 不同产地三叶木通中总黄酮含量的测定[J]. 光谱实验室, 2012, 29(3): 1 345-1 350.
[12] 张孟琴. 三叶木通果皮中果胶和黄酮的提取分离研究[D]. 长沙: 湖南农业大学, 2007.
[13] 邵显会. 三叶木通总黄酮和多糖的提取纯化及生物活性研究[D]. 西安: 陕西师范大学, 2012.
[14] 周燕芳, 丁利君. 超声波辅助提取艾叶黄酮的工艺研究[J]. 食品与机械, 2006, 22(4): 39-41.
[15] 成晓霞, 孙蓉蓉, 欧阳浩楠, 等. 三叶木通中常春藤皂苷的提取工艺[J]. 安徽农业科学, 2008, 36(16): 6 792-6 794.
[16] ZOU Yan-ping, CHANG S K C, GU Yan, et al. Antioxidant activity and phenolic compositions of lentil (Lens culinaris var. Morton) extract and its fractions[J]. Journal of Agricultural & Food Chemistry, 2011, 59(6): 2 268-2 276.
[17] ZIELINSKI A A F, HAMINIUK C W I, ALBERTI A, et al. A comparative study of the phenolic compounds and the in vitro, antioxidant activity of different Brazilian teas using multivariate statistical techniques[J]. Food Research International, 2014, 60(6):246-254.
[18] GORJANOVIC S, KOMES D, PASTOR F T, et al. Antioxidant capacity of teas and herbal infusions: polarographic assessment.[J]. Journal of Agricultural & Food Chemistry, 2012, 60(38):9 573-9 580.
[19] OH J, JO H, CHO A R, et al. Antioxidant and antimicrobial activities of various leafy herbal teas[J]. Food Control, 2013, 31(2): 403- 409.
[20] LU Xiao-nan, ROSS C F, POWERS J R, et al. Determination of total phenolic content and antioxidant activity of garlic (Allium sativum) and elephant garlic (Allium ampeloprasum) by Attenuated total reflectance-fourier transformed infrared spectroscopy[J]. Journal of Agricultural & Food Chemistry, 2011, 59(10): 5 215-5 221.
[21] THI N D, HWANG E S. Effects of drying methods on contents of bioactive compounds and antioxidant activities of black chokeberries ( Aronia melanocarpa )[J]. Food Science & Biotechnology, 2016, 25(1): 55-61.
[22] WANG Da-wei, WANG Jin, LIU Yun-tao, et al. Roles of Chinese herbal medicines in ischemic heart diseases (IHD) by regulating oxidative stress[J]. International Journal of Cardiology, 2016, 220: 314-319.
[23] 周佳, 阮征, 江波, 等. 蔬菜抗氧化能力及与酚酸和总黄酮相关性研究[J]. 食品与机械, 2012, 28(3): 139-143.

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