Optimization of purification technology for total flavonoids from Mentha haplocalyx by macroporous resin

Authors

LIU Xuehui, College of Horticulture and Landscape, Hunan agricultural university, Changsha, Hunan 410128, China; State Key Laboratory of Subhealth Intervention Technology, Changsha, Hunan 410128, China
ZHANG Si, College of Horticulture and Landscape, Hunan agricultural university, Changsha, Hunan 410128, China; State Key Laboratory of Subhealth Intervention Technology, Changsha, Hunan 410128, China
ZHANG Zhixu, College of Horticulture and Landscape, Hunan agricultural university, Changsha, Hunan 410128, China; State Key Laboratory of Subhealth Intervention Technology, Changsha, Hunan 410128, China
KANG Xincong, College of Horticulture and Landscape, Hunan agricultural university, Changsha, Hunan 410128, China; State Key Laboratory of Subhealth Intervention Technology, Changsha, Hunan 410128, China
LI Jian, State Key Laboratory of Subhealth Intervention Technology, Changsha, Hunan 410128, China
XIE Hongqi, College of Horticulture and Landscape, Hunan agricultural university, Changsha, Hunan 410128, China; State Key Laboratory of Subhealth Intervention Technology, Changsha, Hunan 410128, China; Hunan Provincial Key Laboratory of Crop Germplasm Innovation and
LIU Dongbo, College of Horticulture and Landscape, Hunan agricultural university, Changsha, Hunan 410128, China; State Key Laboratory of Subhealth Intervention Technology, Changsha, Hunan 410128, China; Hunan Provincial Key Laboratory of Crop Germplasm Innovation and

Abstract

Based on the adsorption-desorption rate and the content of total flavonoids, static and dynamic adsorption methods were adopted to evaluate the purification ability of 6 macroporous resins, including ADS-7, ADS-17, NKA-9, AB-8, D101, and HPD-100, for purifying total flavonoids from of Mentha haplocalyx. The results showed that AB-8 was adsorbed best among all the candidates. The optimum purification condition was as follows. The flow rate was 1 mL/min, and the loading volume of sample was 3 BV containing flavonoids 2.56 mg/mL in it, desorbed by 4 BV 30% ethanol. Finally, 90.35% total flavonoids from Mentha haplocalyx were obtained. The method above is characteristic of high separation efficiency, convenient operation and reliable stability. This study could provide theory support for the comprehensive utilization of Mentha haplocalyx.

Publication Date

8-28-2016

First Page

156

Last Page

158,177

DOI

10.13652/j.issn.1003-5788.2016.08.038

Recommended Citation

Xuehui, LIU; Si, ZHANG; Zhixu, ZHANG; Xincong, KANG; Jian, LI; Hongqi, XIE; and Dongbo, LIU (2016) "Optimization of purification technology for total flavonoids from Mentha haplocalyx by macroporous resin," Food and Machinery: Vol. 32: Iss. 8, Article 38.
DOI: 10.13652/j.issn.1003-5788.2016.08.038
Available at: https://www.ifoodmm.cn/journal/vol32/iss8/38

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