Optimization on saponin extraction from Rhizoma paridis by inner ebullition

Authors

YUAN Zhihui, Key Laboratory of Comprehensive Utilization of Advantage Plants Resources in Hunan South, Yongzhou, Hunan 425199, China; College of Chemistry and Bioengineering, Hunan University of Science and Engineering, Yongzhou, Hunan 425199, China
JIANG Qiongfeng, Key Laboratory of Comprehensive Utilization of Advantage Plants Resources in Hunan South, Yongzhou, Hunan 425199, China; College of Chemistry and Bioengineering, Hunan University of Science and Engineering, Yongzhou, Hunan 425199, China

Abstract

In order to improve the unit yields of medicinal components, and provide a scientific basis for effective exploring of Rhizoma paridis, single factor experiments were combined with inner ebullition and response surface methods to extract the saponin from Rhizoma paridis. Based on the single factor experiments of ethanol concentration, temperature, liquid feed ratio, and extraction time, the best levels of all factors were established. The extraction conditions were optimized by the Box-Behnken design and the optimum conditions were as follows: 39% of ethanol concentration, 86 ℃ of extraction temperature, and 5.5 min of extraction time. The yield of saponin extracted under the optimal conditions reached 11.02%. The present strategy is simple, rapid and efficient.

Publication Date

8-28-2016

First Page

178

Last Page

181

DOI

10.13652/j.issn.1003-5788.2016.08.043

Recommended Citation

Zhihui, YUAN and Qiongfeng, JIANG (2016) "Optimization on saponin extraction from Rhizoma paridis by inner ebullition," Food and Machinery: Vol. 32: Iss. 8, Article 43.
DOI: 10.13652/j.issn.1003-5788.2016.08.043
Available at: https://www.ifoodmm.cn/journal/vol32/iss8/43

References

[1] JIN Ling-yu, LU Ting-xiang, QIN Xu-jie, et al. Two new highly oxygenated spirostanol saponins from Paris polyphylla var. stenophylla[J]. Natural Products and Bioprospecting, 2016, 6(4): 1-6.
[2] 王羽, 张彦军, 高文远, 等. 滇重楼的抗肿瘤活性成分研究[J]. 中国中药杂志, 2007, 32(14): 1 425-1 428.
[3] 袁晓, 袁萍, 严海燕, 等. 野生珍稀药用植物七叶一枝花的成分含量分析[J]. 武汉植物学研究, 2004, 22(6): 575-577.
[4] DENG Da-wei, LAUREN DR, COONEY JM. et al. Antifungal saponins from Paris polyphylla smith[J]. Planta Medica, 2008, 74(11): 1 397-1 402.
[5] MAN Shu-li, GAO Wen-yuan, ZHANG Yan-jun, et al. Antitumor and antimetastatic activities of Rhizoma Paridis saponins[J]. Steroids, 2009, 74(13/14): 1 051-1 056.
[6] 武姗姗, 高文远, 段宏泉, 等. 重楼化学成分和药理作用研究进展[J]. 中草药, 2004, 35(3): 344-347.
[7] 张娟, 路金才. 皂苷的提取方法及含量测定研究进展[J]. 中国现代中药, 2006, 8(3): 25-28.
[8] 李于善, 周强, 张玉针, 等. 三峡七叶一枝花薯蓣皂苷元的分离提取工艺研究[J]. 化学世界, 2009, 50(2): 86-89.
[9] 王丹辉, 李刚, 张静, 等. 七叶一枝花酊提取工艺研究[J]. 中国药师, 2013, 16(9): 1 364-1 366.
[10] 柳昌武. 辣椒内部沸腾提取及其缓释制剂工艺优化的研究[D]. 南宁: 广西大学, 2012: 19-21.
[11] 唐华丽, 熊汉国, 王玮. 响应面法优化葡萄籽多酚提取工艺[J]. 食品与机械, 2012, 28(6): 147-149, 163.
[12] 郑菲, 黄亮, 李安平, 等. 响应面法优化橡实壳中多酚提取工艺条件[J]. 食品与机械，2011, 27(1): 49-51.
[13] 喻祖文, 刘颖新, 李辉, 等. 微波法提取重楼总皂苷的工艺研究[J]. 时珍国医国药, 2013, 24(4): 833-834.
[14] 郑韵英, 童张法, 韦藤幼. 内部沸腾法提取银叶树叶总黄酮的研究[J]. 广西大学学报: 自然科学版, 2013, 38(3): 527-531.
[15] 余响华, 蒋琼凤, 邵金华. 响应曲面优化喜树生物碱醇提工艺研究[J]. 天然产物研究与开发, 2014, 26(11): 1 858-1 862.