The analyses on chemical components in the leaf volatile oils of Zephyranthes candida（Lindl.）Herb. and their antimicrobial activities in the fresh milk

Authors

WEI Qiang, Pharmacy School, Anhui Xinhua University, Hefei, Anhui 230088, China
SHI Xuefeng, Pharmacy School, Anhui Xinhua University, Hefei, Anhui 230088, China

Abstract

Two kinds of volatile oil were extracted by steam distillation extraction (SDE) and supercritical fluid extraction of CO2 (SFE-CO2), and then they were identified by gas chromatography-mass spectrometry (GC—MS). The antimicroble effects of the extract on different rot funguses in fresh milk was evaluated in 4 ℃ within 10 days. There were 39 different components in the two kinds of volatile oil, including methyl cyclohexane, octacosane, 1,3-dimethyl-benzene, and dibutyl phthalate mainly found in the SDE volatile oil, and 4-(4-ethylcyclohexyl)-1-pentyl-cyclohexene, toluene, 1,2-benzenedicar-boxylic acid, diisooctyl ester, 1-ethoxy-pentane, 2-methoxy-4-vinylphenol, and 2,4-bis(1,1-dimethylethyl)-phenol mostly presented in the SFE-CO2 volatile oil. The differences among the contents of 20 common components in two oils was great. The SFE-CO2 volatile oil showed more effective antimicrobial activity with the MIC range of 0.031～0.125 mg/mL. Moreover, those from SFE-CO2 volatile oil coulld also provide better exotic perfume and stronger antiseptic functions.

Publication Date

8-28-2016

First Page

21

Last Page

24,90

DOI

10.13652/j.issn.1003-5788.2016.08.006

Recommended Citation

Qiang, WEI and Xuefeng, SHI (2016) "The analyses on chemical components in the leaf volatile oils of Zephyranthes candida（Lindl.）Herb. and their antimicrobial activities in the fresh milk," Food and Machinery: Vol. 32: Iss. 8, Article 6.
DOI: 10.13652/j.issn.1003-5788.2016.08.006
Available at: https://www.ifoodmm.cn/journal/vol32/iss8/6

References

[1] 王孝荣, 潘年龙, 罗佳丽. 牛奶保鲜技术的研究进展[J]. 饮料工业, 2012, 15(8): 2-5.
[2] 张晓梅. 鲜牛奶中细菌的分离纯化及其药物敏感性分析[J]. 饲料工业, 2012, 33(5): 26-30.
[3] 王琳, 刘聚祥, 马亚斌, 等. 牛奶防腐剂的研究进展[J]. 中国乳品工业, 2015, 43(5): 43-45.
[4] 吕娜, 沈明浩. 食品防腐剂苯甲酸钠的蓄积毒性及精子毒性研究[J]. 毒理学杂志, 2011, 25(3): 241-242.
[5] 高增兵, 余冰, 郑萍, 等. 苯甲酸对仔猪肠道微生物及代谢产物的影响[J]. 动物营养学报, 2014, 26(4): 1 044-1 054.
[6] SONI M G, CARABIN I G, BURDOCK G A. Safety assessment of esters of p-hydroxybenzoic acid(parabens)[J]. Food Chem Toxicol, 2005, 43(1): 985-1015.
[7] 王步江, 刘金福, 樊秀花, 等. 肉桂精油抑菌活性研究[J]. 食品与机械, 2011, 27(6): 166-167, 182.
[8] 李巧巧, 雷激, 唐洁, 等. 商品柑橘精油的抑菌性及其应用研究[J]. 食品与机械, 2011, 27(6): 160-162.
[9] 邓祖丽颖, 袁秀云. 葱兰的离体培养及再生体系研究[J]. 中国农学通报, 2013, 29(13): 140-144.
[10] 董博, 刘雪, 付春. 葱兰和韭兰花色色素种类和质量分数的研究[J]. 西南大学学报: 自然科学版, 2014, 36(3): 34-41.
[11] 杨简赛, 冯煦, 陈雨, 等. 葱莲鳞茎化学成分研究[J]. 中药材, 2010, 33(11): 1 730-1 732.
[12] NAKAYAMA M, HORIE T, TSUKAYAMA M, et al. Isolation of kaempferol-3-O-rhamnoglucoside, a flavonoid glycoside from Zephyranthes Candida[J]. Zeitschrift Für Naturforschung C, 2014, 33(7/8): 587-588.
[13] 吕辉. 玉帘凝集素的分离纯化、部分性质研究及分子克隆[D]. 成都: 四川大学, 2003: 9-21.
[14] 毕淑峰, 张铃杰, 徐峤, 等. 葱兰花挥发油的化学成分及对自由基的清除作用研究[J]. 北京联合大学学报, 2015, 29(1): 14-18.
[15] MUTSUGA M, KOJIMA K, NOSE M, et al. Cytotoxic activities of alkaloids from Zephyranthes candida[J]. Natural Medicine, 2001, 55(4): 201-204
[16] 卫强, 李前荣, 尹浩, 等. 超临界CO₂萃取法与水蒸气蒸馏法提取垂丝海棠叶挥发油成分及其抗氧化活性的比较研究[J]. 中成药, 2015, 37(11): 2 550-2 554.
[17] 卫强, 纪小影. 红叶李的叶、茎挥发油成分GC—MS分析及体外抗菌、抗病毒活性研究[J]. 中药新药与临床药理, 2016, 27(2): 263-268.
[18] 刘劲芸, 阴耕云, 张虹娟, 等. 超临界CO₂萃取与同时蒸馏萃取法提取澳洲坚果花挥发性成分研究[J]. 云南大学学报: 自然科学版, 2013, 35(5): 678-684.
[19] 徐敬宜, 徐永平, 刘姝, 等. 溶菌酶及其在食品工业中的应用[J]. 食品与机械, 2006, 22(1): 90-92.
[20] 吕世明, 陈杖榴, 陈建新, 等. 丁香酚体外抑菌作用研究[J]. 食品科学, 2008, 29(9): 122-124.
[21] 唐小辉. 肉桂醛、丁香酚及其结构类似物的抑菌活性与化学结构的关系[D]. 湘潭: 湘潭大学, 2013: 35-53.