•  
  •  
 

Abstract

In order to clarify the flavonoids compositions of rape bee pollen and evaluate the assisted extraction effect for pulsed electric field (PEF), the ethanol extract of rape bee pollen and its acidolysis component were analyzed in detail by the method of TLC—DPPH and HPLC in this paper. The results showed that the quercetin and kaempferol in the ethanol extract of rape bee pollen could not be detected for their low contents. However, a large number of quercetin and kaempferol were generated after the acidolysis of ethanol extract, and this implied that the flavonoids compositions of rape bee pollen mainly existed in the form of flavonoid glycoside. In addition, the content of quercetin and kaempferol in the acidolysis of ethanol extract were 764.39 μg/g and 1 647.97 μg/g respectively when the acidolysis materials were not treated by PEF. Nevertheless, when the acidolysis of ethanol extract was pretreated by the PEF afterwards, the content of quercetin and kaempferol reached 867.93 μg/g and 1 924.03 μg/g, increasing by 13.55% and 16.75% respectively. These results fully proved that the PEF technology pretreatment was an effective assisted extraction method and could be conducive to break the walls for the rape bee pollen cells.

Publication Date

9-28-2016

First Page

144

Last Page

148

DOI

10.13652/j.issn.1003-5788.2016.09.036

References

[1] 王开发, 耿越. 花粉中黄酮类研究[J]. 养蜂科技, 1997(3): 8-12.
[2] 杨洁, 陈纯, 邢建军, 等. 油菜蜂花粉中黄酮类化合物的提取与鉴定[J]. 食品科学, 2010, 31(22): 273-278.
[3] 李军, 横田正实. 油菜蜂花粉的黄酮化学成分研究[J]. 浙江省医学科学院学报, 1990(3): 14-17.
[4] SERRA B J, SOLIVA T M, CENTELLES L E. Evaluation of polyphenolic and flavonoid compounds in honeybee-collected pollen produced in Spain.[J]. Journal of Agricultural & Food Chemistry, 2001, 49(4): 1 848-1 853.
[5] 杨必成, 刘海, 杨义芳, 等. 油菜花粉中黄酮类化合物的提取与分析[J]. 中草药, 2011, 42(12): 2 451-2 455.
[6] MONTEIRO M L G, MRSICO E T, LZARO C A, et al. Thin-layer chromatography applied to foods of animal origin: a tutorial review[J]. Journal of Analytical Chemistry, 2016, 71(5): 459-470.
[7] TANG Tie-xin, XU Xin-jun, WANG Dong-mei, et al. A rapid and green limit test method for five synthetic colorants in foods using polyamide thin-layer chromatography[J]. Food Analytical Methods, 2014, 8(2): 459-466.
[8] UKASZ Ciesla, KRYSZEN J, STOCHMAL A, et al. Approach to develop a standardized TLC-DPPH, test for assessing free radical scavenging properties of selected phenolic compounds[J]. Journal of Pharmaceutical & Biomedical Analysis, 2012, 70: 126-135.
[9] 杨佳林, 孙丽萍, 徐响, 等. 油菜蜂花粉黄酮醇的测定及其抗氧化活性研究[J]. 食品科学, 2010, 31(3): 79-82.
[10] 郭金凤, 张俊妹, 张芳, 等. TLC-生物自显影法筛选榆叶合叶子的抗氧化活性[J]. 中国民族民间医药, 2013, 22(24): 9-10.
[11] CHAAIB F, QUEIROZ E F, NDJOKO K, et al. Antifungal and antioxidant compounds from the root bark of Fagara zanthoxyloides.[J]. Planta Medica, 2003, 69(4): 316-320.
[12] 任向楠. 超声波辅助酶法破壁油菜花粉的研究[D]. 北京: 中国农业科学院, 2010: 64-90.
[13] 王芙蓉. 油菜花粉的破壁方法及其多糖、黄酮提取纯化工艺研究[D]. 武汉: 华中农业大学, 2007: 28-29.
[14] 阮征, 胡筱波, 赖富饶, 等. 油菜花粉中黄酮类物质提取工艺的优化研究[J]. 食品科学, 2007, 28(7): 133-137.
[15] 熊夏宇, 曾新安, 王满生, 等. 响应面法优化脉冲电场辅助提取油菜花粉中黄酮类物质工艺研究[J]. 中国农业科技导报, 2015(5): 88-93.
[16] 刘玲玲, 杨辉. HPLC法同时测定银杏叶提取物两种不同剂型中黄酮类化学成分的含量[J]. 青岛医药卫生, 2011, 43(6): 404-406.
[17] 谷丽华, 侴桂新, 王峥涛. 基于ABTS自由基的薄层色谱—生物自显影新方法的建立及其在中药抗氧化活性评价中的应用[J]. 上海中医药大学学报, 2013(3): 99-104.

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.