Abstract
To detect infant complementary food supplements of folic acid, methds of microbiological method, solid-phase-extraction-HPLC and direct-ammonia-extraction-HPLC were utilized respectively. Analyses among the three methods were compared and the most accurate and rapid one was determined. The results showed the direct-ammonia-extraction-HPLC analysis by using the CH3OH-0.05 mol/L KH2PO4 (volum ratio,5∶95; pH 6.3) as mobile phase and controlling the peak time at 6.5 min, and the standard curve coefficient was 0.999 8 with detection limit 0.019 0 mg/L. Compared with the other two methods, this method was simple, rapid, sensitive and reproducible characteristics, and provided an effective theoretical basis to establish method of folic acid detections in infant complementary food supplements.
Publication Date
9-28-2016
First Page
34
Last Page
38
DOI
10.13652/j.issn.1003-5788.2016.09.008
Recommended Citation
Minting, LUO; Kegang, WU; and Weiqian, HUANG
(2016)
"Methods of detecting the content of folic acid in infant complementary food supplements,"
Food and Machinery: Vol. 32:
Iss.
9, Article 8.
DOI: 10.13652/j.issn.1003-5788.2016.09.008
Available at:
https://www.ifoodmm.cn/journal/vol32/iss9/8
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