Ultrasonic-assisted extraction and physicochemical characteristics of collagen from rabbit-skin

Authors

ZHANG Xiaojie, College of Food Science, Southwest University, Chongqing 400715, China
ZHANG Yuhao, College of Food Science, Southwest University, Chongqing 400715, China
MA Liang, National Food Science and Engineering Experimental Teaching Center, Southwest University, Chongqing 400715, China
MA Mingsi, National Food Science and Engineering Experimental Teaching Center, Southwest University, Chongqing 400715, China
ZHENG Hong, National Food Science and Engineering Experimental Teaching Center, Southwest University, Chongqing 400715, China
SUN Yi, National Food Science and Engineering Experimental Teaching Center, Southwest University, Chongqing 400715, China

Abstract

The application of ultrasonic irradiation to extract pepsin-soluble collagen from the skins of rabbit was investigated in this study. The results showed that the extraction rate of collagen using the ultrasonic irradiation was (83.69±0.51) % and the yield increased by 25% in comparison with the conventional pepsin isolation method. The result of polyacrylamide gel electrophoresis confirmed that the subunits structure of collagen using the ultrasonic irradiation was mainly of α1, α2 and β, without subunit degradation. Moreover, the ultraviolet spectrum exhibited a typical absorption peak at 217 nm, indicating that it greatly maintained the characteristics of type I collagen. Additionally, the Fourier transform infrared spectroscopy revealed that hydrogen bond was partially destroyed, but the triple helix structure of collagen remained intact even after the ultrasonic irradiation. However, the result of Scanning Differential Calorimeter analyses showed that the thermal denaturation temperature and enthalpy value of collagen using the ultrasonic irradiation were slightly lower than that of the traditional rabbit collagen, and it still had a relatively higher thermal stability.

Publication Date

1-28-2017

First Page

167

Last Page

171

DOI

10.13652/j.issn.1003-5788.2017.01.038

Recommended Citation

Xiaojie, ZHANG; Yuhao, ZHANG; Liang, MA; Mingsi, MA; Hong, ZHENG; and Yi, SUN (2017) "Ultrasonic-assisted extraction and physicochemical characteristics of collagen from rabbit-skin," Food and Machinery: Vol. 33: Iss. 1, Article 38.
DOI: 10.13652/j.issn.1003-5788.2017.01.038
Available at: https://www.ifoodmm.cn/journal/vol33/iss1/38

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