Optimization of technological conditions for enzymatic hydr olysis of sweet corn pulp

Authors

LU Manxia, Faculty of Environmental and Biological Engineering, Guangdong University of Petrochemical Technology, Maoming, Guangdong 525000, China)
ZHANG Ling, Faculty of Environmental and Biological Engineering, Guangdong University of Petrochemical Technology, Maoming, Guangdong 525000, China)
LIN Zhisang, Faculty of Environmental and Biological Engineering, Guangdong University of Petrochemical Technology, Maoming, Guangdong 525000, China)
WANG Zeqi, Faculty of Environmental and Biological Engineering, Guangdong University of Petrochemical Technology, Maoming, Guangdong 525000, China)
HUANG Xiaocui, Faculty of Environmental and Biological Engineering, Guangdong University of Petrochemical Technology, Maoming, Guangdong 525000, China)
XIE Huxi, Faculty of Environmental and Biological Engineering, Guangdong University of Petrochemical Technology, Maoming, Guangdong 525000, China)

Abstract

The sweet corn juice was prepared by using double enzymes method, i.e. catalyzed by medium temperature α-amylase and saccharifying enzyme. The soluble solid matter content was analyzed as the basis of evaluation. The liquefaction process was investigated in solid-liquid ratio, medium temperature α-amylase dosage, enzymatic hydrolysis temperature and time on the amylolysis effect, and then the effects of the amount of saccharifying enzyme, saccharification temperature and time on the enzyme solution were also studied. On the basis of single factor experiments, the optimum technological conditions of double enzyme hydrolysis for sweet corn were optimized by orthogonal experiment. The results showed that the sweet corn pulp could be produced best, using the solid-liquid ratio of 14 (g/mL) and liquefied at 55 ℃for 25 min with 0.35% of medium temperature α-amylase, then saccharified for 20 min with 0.15% of saccharifying enzyme. The soluble solid matter content of the sweet corn juice prepared under the best condition was 3.90%. This method showed the advantages of simple operation, consuming short time, and effective juice extraction, etc., and it could be popularized and applied in the processing of sweet corn beverage.

Publication Date

1-28-2017

First Page

188

Last Page

192

DOI

10.13652/j.issn.1003-5788.2017.01.042

Recommended Citation

Manxia, LU; Ling, ZHANG; Zhisang, LIN; Zeqi, WANG; Xiaocui, HUANG; and Huxi, XIE (2017) "Optimization of technological conditions for enzymatic hydr olysis of sweet corn pulp," Food and Machinery: Vol. 33: Iss. 1, Article 42.
DOI: 10.13652/j.issn.1003-5788.2017.01.042
Available at: https://www.ifoodmm.cn/journal/vol33/iss1/42

References

[1] 王孝华, 曹祖波. 我国玉米生产现状及发展策略[J]. 农业科技通讯, 2015(5): 5-6.
[2] 杨婧曦, 于海峰. 酶法制备白果甜玉米澄清饮料的研究[J]. 食品科技, 2013(9): 76-78.
[3] 刘晓涛. 甜玉米的营养价值及其加工现状的研究[J]. 技术装备, 2009(3): 47-48.
[4] 刘蔚楠, 万忠, 甘阳英, 等. 2015年广东甜玉米产业发展形势与对策建议[J]. 广东农业科学, 2016(3): 12-16.
[5] 诚夫. 世界饮料发展趋势[J]. 江西食品工业, 2002(3): 48.
[6] 2012年全国饮料总产量为13 024.01万吨[J]. 饮料工业, 2013, 16(2): 10.
[7] 2012年1-12月全国饮料分月总产量及与上年同比[J]. 饮料工业, 2013, 16(3): 50-52.
[8] 谢红涛, 余瑞婷, 赵瑞娟, 等. 果蔬汁加工技术进展[J]. 农产品加工学刊, 2010, 1(1): 76-80.
[9] 徐玉娟, 肖更生, 李升锋, 等. 甜玉米汁酶解工艺条件的研究[J]. 广东农业科学, 2006(11): 48-50.
[10] 李次力, 缪铭. 酶解甜玉米糖化液开发营养性饮料的研究[J]. 食品科学, 2005, 26(5): 175-178.
[11] 邓开野, 麦伟浩. 甜玉米饮料的加工工艺研究[J]. 广西轻工业, 2009(2): 1-2.
[12] 陶兴无. α-淀粉酶在甜玉米饮料生产中的应用研究[J]. 湖北工业大学学报, 2006, 21(2): 17-20.
[13] 罗映英, 宋小峰, 王凤仙, 等. 甜玉米饮料的工艺配方优化[J]. 农产品工程, 2015(7): 55-57.