Abstract
Lactic acid bacteria (LAB) with ability of acid and bile salt resistance were isolated from the feces of the longevity population in Rugao by using selective medium, artificial gastric juice and bile salt medium, and the ability of cholesterol reducing and manner were studied. The results showed that the survival rate of all isolated 60 strains of LAB in the pH 3.0 artificial gastric juice was more than 30%, the LAB from the middle-aged group, elderly group and longevity group was significantly higher than that in the youth group (P＜0.05); the survival rate of LAB in the 0.3% and 0.5% bile salt culture medium was more than 20%, of which the LAB in elderly group and longevity group was significantly higher than that of youth group and middle-aged group (P＜0.05). The ability of cholesterol lowering of the LAB from the longevity group was significantly higher than that of middle-aged group (P＜0.05), the ability of cholesterol reducing of lactobacillus spp. and Weissella spp. was significantly higher than that of Enterococcus spp. (P＜0.05), the main manners of cholesterol lowering of LAB were cell absorption and sedimentation. The LAB come from longevity population have the strong ability of acid and bile salt resistance and cholesterol lowering, and the longevity group are higher than others from other age groups.
Publication Date
10-28-2017
First Page
1
Last Page
5
DOI
10.13652/j.issn.1003-5788.2017.10.001
Recommended Citation
Dawei, CHEN; Ruixia, GU; Maolin, LU; Yujun, HUANG; Chenchen, ZHANG; Chengran, GUAN; Yulu, ZHANG; and Chenyu, FAN
(2017)
"Ability of acid and bile salt resistance of lactic acid bacteria and its cholesterol lowering effect from human origin,"
Food and Machinery: Vol. 33:
Iss.
10, Article 1.
DOI: 10.13652/j.issn.1003-5788.2017.10.001
Available at:
https://www.ifoodmm.cn/journal/vol33/iss10/1
References
[1] WANG D Q. Regulation of intestinal cholesterol absorption[J]. Annual Review of Physiology, 2007, 69: 221-248.
[2] LEE C M Y, BARZI F, WOODWARD M, et al. Adult height and the risks of cardiovascular disease and major causes of death in the Asia-Pacific region: 21 000 deaths in 510 000 men and women[J]. International Journal of Epidemiology, 2009, 38(4): 1 060-1 071.
[3] BOGERS R P, BEMELMANS W J, HOOGENVEEN R T, et al. Association of overweight with increased risk of coronary heart disease partly independent of blood pressure and cholesterol levels: a meta-analysis of 21 cohort studies including more than 300 000 persons[J]. Archives Internal Medicine, 2007, 167(16): 1 720-1 728.
[4] JONES M L, MARTONI C J, PARENT M, et al. Cholesterol-lowering efficacy of a microencapsulated bile salt hydrolase-active Lactobacillus reuteri NCIMB 30242 yoghurt formulation in hypercholesterolaemic adults[J]. British Journal of Nutrtion, 2012, 107(10): 1 505-1 513.
[5] 高权新, 吴天星, 王进波. 肠道微生物与寄主的共生关系研究进展[J]. 动物营养学报, 2010, 22(3): 519-526.
[6] MARIA S, GUNNAR M, RANGNE F, et al. Probiotic bacteria: safety, functional and technological properties[J]. Journal of Biotechnology, 2001, 84(3): 197-215.
[7] LYE H S, RAHMAT-ALI G R, LIONG M T. Mechanisms of cholesterol removal by lactobacilli under conditions that mimic the human gastrointestinal tract[J]. International Dairy Journal, 2010, 20(3): 169-175.
[8] LYE H S, RUSUL G, LIONG M T. Removal of cholesterol by lactobacilli via incorporation and conversion to coprostanol[J]. Journal of Dairy Science, 2010, 93(4): 1 383-1 392.
[9] 麦热姆妮萨·艾麦尔. 长寿老人肠道益生菌的分离鉴定及长寿机理的研究[D]. 乌鲁木齐: 新疆大学, 2012: 22-23.
[10] 王俊国. 降胆固醇益生乳杆菌的筛选及其降胆固醇作用的研究[D]. 呼和浩特: 内蒙古农业大学, 2008.
[11] 刘丽莉, 夏延斌, 唐青春. 降胆固醇的乳酸菌筛选研究[J]. 食品科学, 2004, 25(7): 59-62.
[12] 王友湘, 陈庆森, 阎亚丽. 用于乳酸菌分离鉴定的几种培养基的筛选及应用[J]. 食品科学, 2007, 28(9): 374-377.
[13] 吕源玲. 耐酸耐胆盐益生乳酸菌的筛选与鉴定[J]. 食品与机械, 2017, 33(6): 42-45.
[14] 王一鸣, 范小兵, 杨虹, 等. 体外去除胆固醇菌株的筛选及其作用机理研究[J]. 微生物学通报, 2006, 33(6): 43-47.
[15] 布坎南 R E, 吉布斯 N E. 伯杰氏细菌鉴定手册[M]. 8版. 中国科学院微生物研究所, 译. 北京: 科学出版社, 1984: 660-721.
[16] HUNGER W, PEITERSEN N. New technical aspects of the preparation of starter culture[J]. Bulletin of the International Dairy Federation, 1992, 277: 17-21.
[17] 李晓华, 李方儒. 老年人空腹胃液pH值和胃蛋白酶观察[J]. 武警医学院学报, 2002, 12(1): 52-54.
[18] KOBAYASHI H. A proton-translocating ATPase regulates pH of the bacterial cytoplasm[J]. The Journal of Biological Chemistry, 1985, 260(1): 72-76.
[19] KOBAYASHI H, SUZUKI T, UNEMOTO T. Streptococcal cytoplasmic pH is regulated by changes in amount and activity of a proton-ranslocating ATPase[J]. The Journal of Biological Chemistry, 1986, 261(2): 627-630.
[20] 柯元南, 张红. 胆固醇的功过是非[J]. 中老年保健, 2017(2): 10-11.
[21] ECKBURG P B, BIK E M, BERNSTEIN C N, et al. Diversity of the human intestinal microbial flora[J]. Science, 2005, 308(5 728): 1 635-1 638.