Enzyme-linked sensitization biosensor detection of bisphenol A in food matrix Based on magnetic nanomaterials

Authors

XU Zhou, School of Chemistry and Biological Engineering, Changsha University of Science & Technology, Changsha, Hunan 410114, China
TANG Yao, School of Chemistry and Biological Engineering, Changsha University of Science & Technology, Changsha, Hunan 410114, China
WANG Rong, School of Chemistry and Biological Engineering, Changsha University of Science & Technology, Changsha, Hunan 410114, China
CHEN Maolong, School of Chemistry and Biological Engineering, Changsha University of Science & Technology, Changsha, Hunan 410114, China
WEN Li, School of Chemistry and Biological Engineering, Changsha University of Science & Technology, Changsha, Hunan 410114, China
CHENG Yunhui, School of Chemistry and Biological Engineering, Changsha University of Science & Technology, Changsha, Hunan 410114, China

Abstract

A horseradish peroxidase enzyme-sensitized detection platform based on functionalized magnetic nanoparticles and gold nanoparticles was established with the nanomaterials as the carrier and the bisphenol A aptamer and the complementary strand as the biometric recognition unit. The method about rapid pretreatment and trace detection of bisphenol A in food matrix was established. The BPA aptamer binding to the magnetic nanomaterials could compete with the complementary strands of the aptamer and the horn root peroxidase (HRP) modified gold nanoparticles. The effect of HRP on the catalytic hydrolysis of the substrate resulted in the change of characteristic peaks at 450 nm, helping to quantitatively detect bisphenol A, which had the advantages of good selectivity, high sensitivity and good specificity. The results showed that this detection system had a linear relationship (R²=0.978 4) in the concentration range of 0～100 ng/mL, and the limit of detection was as low as 0.5 pg/mL. This work provideed strong technical support for detection of bisphenol A in future.

Publication Date

10-28-2017

First Page

47

Last Page

51

DOI

10.13652/j.issn.1003-5788.2017.10.010

Recommended Citation

Zhou, XU; Yao, TANG; Rong, WANG; Maolong, CHEN; Li, WEN; and Yunhui, CHENG (2017) "Enzyme-linked sensitization biosensor detection of bisphenol A in food matrix Based on magnetic nanomaterials," Food and Machinery: Vol. 33: Iss. 10, Article 10.
DOI: 10.13652/j.issn.1003-5788.2017.10.010
Available at: https://www.ifoodmm.cn/journal/vol33/iss10/10

References

[1] 代欢欢. 基于HPLC法对罐装食品中双酚A的测定方法研究[J]. 食品与机械, 2016, 32(3): 93-95.
[2] ALONSO-MAGDALENA P, ROPERO A B, SORIANO S, et al. Bisphenol-A: a new diabetogenic factor [J]. Hormones, 2010, 9(2): 118-126.
[3] 刘肖. 仪器分析技术在矿泉水及包装材料分析中的新进展[J]. 中国食品工业, 2011(12): 24-26.
[4] 张彦丽, 任佳丽, 李忠海, 等. 食品包装材料中双酚 A 的研究进展[J]. 食品与机械, 2011, 27(1): 155-157.
[5] SHARP L, BLACK R J, MUIR C S, et al. Will the Scottish Cancer Target for the year 2000 be met? The use of cancer registration and death records to predict future cancer incidence and mortality in Scotland[J]. British Journal of Cancer, 1996, 73(9): 1 115-1 121.
[6] MERCEA P. Physicochemical process involved in migration of bisphenol A from poly carbon[J]. Applied Polymer Science, 2009, 112(2): 579-593.
[7] HEGNEROV K, HOMOLA J. Surface plasmon resonance sensor for detection of bisphenol A in drinking water[J]. Sensors and Actuators B: Chemical, 2010, 151(1): 177-179.
[8] 刘银梅, 沈月平, 梁红, 等. 双酚A与复发性流产的相关性[J].中华预防医学杂志, 2011, 5(4): 344-349.
[9] TAKAO T, NANAMIYA W, NAGANO I, et al. Exposure with the environmental estrogen bisphenol a disrupts the male reproductive tract in young mice[J]. Life Sciences, 1999, 65(22): 2 351-2 357.
[10] 郭伟鹏, 吴清平, 梁达清, 等. 聚碳酸酯饮用水桶中双酚A迁移量的研究[J]. 食品与机械, 2014, 30(1): 78-81.
[11] 邓茂先, 吴德生, 陈祥贵, 等. 双酚A雄性生殖毒性的体内外实验研究[J]. 中华预防医学杂志, 2004, 38(6): 383-387.
[12] 刘蕾, 王彦军, 高虹, 等. 职业暴露妇女血清、尿液双酚A水平与乳腺癌关系的研究[J]. 中国公共卫生管理, 2012(6): 845-847.
[13] 杨淋清, 庄志雄. 低剂量双酚A与乳腺癌关系研究进展[J]. 中国公共卫生, 2010, 26(12): 1 557-1 559.
[14] ABDI Hamidreza, POURMALEK Farshad, ZARGAR Homayoun, et al. Multiparametric magnetic resonance imaging enhances detection of significant tumor in patients on active surveillance for prostate cancer[J]. Urology, 2015, 85(2): 423-429.
[15] ANA B G, SOLEDAD R, DOLORES P B. Analytical methods for the determination of bisphenol A in food.[J]. Journal of Chromatography A, 2009, 1 216(3): 449-469.
[16] STUART J D, CAPULONG C P, LAUNER K D, et al. Analyses of phenolic endocrine disrupting chemicals in marine samples by both gas and liquid chromatography-mass spectrometry[J]. Journal of Chromatography A, 2005, 1 079(s1/2): 136-145.
[17] BRAUNRATH R, CICHNA M. Sample preparation including sol-gel immunoaffinity chrom-atography for determination of bisphenol A in canned beverages, fruits and vegetables[J]. Journal of Chromatography A, 2005, 1 062(2): 189-198.
[18] 李丽莎, 杨湘霞, 汪莉. 高效液相色谱法检测桶装饮用水中双酚A[J]. 实用预防医学, 2006,13(2): 429-430.
[19] IMANAKA M, SASAKI K, NEMOTO S, et al. Determination of bisphenol A in foods using GC/MS[J]. Journal of the Food Hygienic Society of Japan, 2001, 42(2): 71-78.
[20] 陈啟荣, 魏岩, 郎爽, 等. 加速溶剂萃取/气相色谱-质谱法测定糖果包装材料中的双酚A[J]. 食品科学, 2010(6): 165-167.
[21] FLOOD H, KNAPP W J. Comparison of Elisa-and LC-MS-based methodologies for the exposure assessment of bisphenol A[J]. Toxicology Mechanisms & Methods, 2006, 16(8): 427-430.
[22] ZHENG Jie, ZHANG Kun, ZHAO Su-qing. Study on Spectral and immune identification of artificial antigen of bisphenol A[J]. Spectroscopy & Spectral Analysis, 2008, 28(7): 1 583-1 586.
[23] ZHAO Mei-ping, LI Yuan-zong, GUO Zhen-quan, et al. A new competitive enzyme-linked immunosorbent assay (ELISA) for determination of estrogenic bisphenols[J]. Talanta, 2002, 57(6): 1 205-1 210.
[24] ZHU Xiao-li, HAN Kun, LI Gen-xi. Magnetic nanoparticles applied in electrochemical detection of controllable DNA hybridization[J]. Analytical Chemistry, 2006, 7(4): 285-289.
[25] HUI W, ERKANG W. Fe₃O₄ magnetic nanoparticles as peroxidase mimetics and their applications in H₂O₂ and glucose detection.[J]. Analytical Chemistry, 2008, 80(6): 2 250-2 254.
[26] TSAI H Y, HSU C F, CHIU I W, et al. Detection of C-reactive protein based on immunoassay using antibody-conjugated magnetic nanoparticles[J]. Analytical Chemistry, 2007, 79(21): 8 416-8 419.
[27] ZHANG Jia-jia, DAI Pei-qing, LI Chao, et al. A symmetrically split g-quadruplex dnazymes biosensor based on magnetic nanoparticles for the rapid detection of Hg²⁺[J]. Acta Chimica Sinica, 2014, 72(9): 1 029-1 035.
[28] TANG Dian-ping, LIU Bing-qian, Niessner R, et al. Targetindu-ced displacement reaction accompanying cargo release from magnetic mesoporous silica nanocontainers for fluorescence immunoassay[J]. Analytical chemistry, 2013, 85(21): 10 589-10 596.
[29] YUNJ S, SUK H J, SUNG H Y, et al. Novel antibody/gold nanoparticle/magnetic nanoparticle nanocomposites for immunomagnetic separation and rapid colorimetric detection of Staphylococcus aureus in milk[J]. Biosensors & Bioelectronics, 2013, 43(1): 432-439.
[30] AMBROSI A, CASTAEDA M T, KILLARD A J, et al. Double-codified gold nanolabels for enhanced immunoanalysis[J]. Analytical Chemistry, 2007, 79(14): 5 232-5 240.