Effect of different extraction methods on the properties and biological activity of polysaccharides from Radix Glehniae

Authors

JING Yongshuai, College of Chemistry and Pharmaceutical Engineering, Hebei University of Science and Technology, Shijiazhuang, Hebei 050018, China
ZHANG Danshen, College of Chemistry and Pharmaceutical Engineering, Hebei University of Science and Technology, Shijiazhuang, Hebei 050018, China
ZHANG Ruijuan, College of Chemistry and Pharmaceutical Engineering, Hebei University of Science and Technology, Shijiazhuang, Hebei 050018, China
SU Lei, College of Chemistry and Pharmaceutical Engineering, Hebei University of Science and Technology, Shijiazhuang, Hebei 050018, China
PAN Mengshu, College of Chemistry and Pharmaceutical Engineering, Hebei University of Science and Technology, Shijiazhuang, Hebei 050018, China
SUN Shuaishuai, College of Chemistry and Pharmaceutical Engineering, Hebei University of Science and Technology, Shijiazhuang, Hebei 050018, China
WU Lanfang, College of Pharmacology, Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050200, China
ZHENG Yuguang, College of Pharmacology, Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050200, China

Abstract

The polysaccharides were extracted from Radix Glehniae, by using hot water extraction, ultrasonic extraction, microwave extraction, lye extraction, acid extraction and cellulase extraction, and the corresponding polysaccharide was GLP-W, GLP-U, GLP-M, GLP-L, GLP-A and GLP-E. The extraction rate, soluble sugar content, viscosity, structural characteristics, solid morphology, antioxidant activity and hypoglycemic activity of each polysaccharide were measured. The results showed: the extraction rates of polysacchar-ides were GLP-A(39.62%)＞GLP-E(38.33%)＞GLP-L(24.80%)＞GLP-W(23.93%)＞GLP-M(18.92%)＞GLP-U(12.68%); The viscosity of the polysaccharide solution by different extraction methods were increased with the increase of polysaccharide solution concentration, and at the same concentration, GLP-L had the largest viscosity. The content of soluble sugar was GLP-A(74.05%)＞GLP-L(67.57%)＞GLP-W(65.41%)＞GLP-U(63.25%)＞GLP-E(33.03%)＞GLP-M(28.71%). The antioxidant activity and the inhibitory activity against α-glucosidase showed that, with the increase of polysaccha-ride concentration, the activity gradually increased. However, when the concentration reached a certain range, the activity tended to be stable. Ultrasonic extraction of R. Glehniae polysaccharide scavenging of DPPH and hydroxyl radicals was the highest, and the IC₅₀ were 3.091 mg/mL and 1.999 mg/mL, respectively; The strongest inhibition of α-glucosidase was obtained by alkali extraction, the IC₅₀ was 1.437 mg/mL.

Publication Date

10-28-2017

First Page

149

Last Page

153

DOI

10.13652/j.issn.1003-5788.2017.10.033

Recommended Citation

Yongshuai, JING; Danshen, ZHANG; Ruijuan, ZHANG; Lei, SU; Mengshu, PAN; Shuaishuai, SUN; Lanfang, WU; and Yuguang, ZHENG (2017) "Effect of different extraction methods on the properties and biological activity of polysaccharides from Radix Glehniae," Food and Machinery: Vol. 33: Iss. 10, Article 33.
DOI: 10.13652/j.issn.1003-5788.2017.10.033
Available at: https://www.ifoodmm.cn/journal/vol33/iss10/33

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