The extraction of finger millet polyphenols by using ultrasound-assisted aqueous two-phase and its antioxidant activity

Authors

WANG Shuanghui, Key Laboratory of Pesticide Hurmless Application, Hunan University of Humanities, Science and Technology, Loudi, Hunan 417000, China; Hunan Provincial Collaborative Innovation Center for Field Weeds Control, Loudi, Hunan 417000, China; College of Agriculture and Biotechnology, Hunan University of Humanities, Science and Technology, Loudi, Hunan 417000, China
ZHANG Li, College of Agriculture and Biotechnology, Hunan University of Humanities, Science and Technology, Loudi, Hunan 417000, China
ZHONG Yuanyuan, College of Agriculture and Biotechnology, Hunan University of Humanities, Science and Technology, Loudi, Hunan 417000, China
JIN Chenzhong, Key Laboratory of Pesticide Hurmless Application, Hunan University of Humanities, Science and Technology, Loudi, Hunan 417000, China; Hunan Provincial Collaborative Innovation Center for Field Weeds Control, Loudi, Hunan 417000, China; College of Agriculture and Biotechnology, Hunan University of Humanities, Science and Technology, Loudi, Hunan 417000, China

Abstract

The ultrasonic-assisted ethanol-ammonium sulfate aqueous two-phase system was adopted to extract polyphenols from finger millet. The extraction process was optimized by single factor test and response surface analysis, polyphenols yield as the index. The results showed that the optimum extraction process was determined as follows: ethanol volume fraction of 41%, ammonium sulfate concentration of 0.3 g/mL, ultrasonic temperature of 42 ℃, liquid-solid ratio of 60 mL/g, and treated for 29 min. By adopting the process, the extraction yield of polyphenols was 319.15 mg/100 g. Finger millet polyphenols had strong antioxidant activity in vitro, the total reducing capacity, DPPH radical scavenging activity, hydroxyl radical scavenging ability reached 91.84%, 96.79% and 89.03% of V_C at the same concentration, respectively.

Publication Date

11-28-2017

First Page

161

Last Page

166,183

DOI

10.13652/j.issn.1003-5788.2017.11.034

Recommended Citation

Shuanghui, WANG; Li, ZHANG; Yuanyuan, ZHONG; and Chenzhong, JIN (2017) "The extraction of finger millet polyphenols by using ultrasound-assisted aqueous two-phase and its antioxidant activity," Food and Machinery: Vol. 33: Iss. 11, Article 34.
DOI: 10.13652/j.issn.1003-5788.2017.11.034
Available at: https://www.ifoodmm.cn/journal/vol33/iss11/34

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