Effect of anti-aging on human epidermal keratinocyte by UVB induced for green tea crude water extract

Authors

WANG Zhen, Key Lab of Tea Science of China Ministry of Education, Hunan Agricultural University, Changsha, Hunan 410128, China
LIU Zhonghua, Key Lab of Tea Science of China Ministry of Education, Hunan Agricultural University, Changsha, Hunan 410128, China; National Research Center of Engineering & Technology for Utilization of Botanical Functional Ingredients, Changsha, Hunan 410128, China; C
CAI Shuxian, Key Lab of Tea Science of China Ministry of Education, Hunan Agricultural University, Changsha, Hunan 410128, China; National Research Center of Engineering & Technology for Utilization of Botanical Functional Ingredients, Changsha, Hunan 410128, China; C
LIU An, Key Lab of Tea Science of China Ministry of Education, Hunan Agricultural University, Changsha, Hunan 410128, China
WEN Yi, Key Lab of Tea Science of China Ministry of Education, Hunan Agricultural University, Changsha, Hunan 410128, China

Abstract

In this study, anti-aging effects of green tea crude water extract were studied using human epidermal keratinocyte as model which induced by ultraviolet B (UVB). The green tea extract of 0～20 μg/mL was added to DMEM cell culture medium, then cells were induced by radiation of 60 mJ/cm² UVB. The anti-aging effects were studied by cellular morphology, apoptosis level, cell viability, intracellular antioxidant enzyme activity, intracellular active oxygen cluster (ROS) content, malonaldehyde（MDA）content, cell mitochondrial membrane potential and Na⁺,K⁺-ATP enzyme activities. The results showed that the crude extract of green tea could obviously improve cell volume, cell shape, and nuclear volume of induced cells. Apoptosis of cells was inhibited, and the survival rate was increased by (30.52±8.69)%. The activity of antioxidant enzyme in cells was obviously improved. ROS content was decreased by 17.16%. The content of MDA was reduced by (50.69±6.81)%. The average light density of red green fluorescence in JC-1 dyeing was increased by 13.75%. Besides, the activity of Na⁺,K⁺-ATP enzyme was increased by (103.95±3.64)%. The experimental results show that green tea extracts have anti-UVB induced aging effect on human epidermal cell, and this study may provide scientific basis to develop anti-aging products of green tea.

Publication Date

12-28-2017

First Page

129

Last Page

134

DOI

10.13652/j.issn.1003-5788.2017.12.026

Recommended Citation

Zhen, WANG; Zhonghua, LIU; Shuxian, CAI; An, LIU; and Yi, WEN (2017) "Effect of anti-aging on human epidermal keratinocyte by UVB induced for green tea crude water extract," Food and Machinery: Vol. 33: Iss. 12, Article 26.
DOI: 10.13652/j.issn.1003-5788.2017.12.026
Available at: https://www.ifoodmm.cn/journal/vol33/iss12/26

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