Study on separation and purification process of fisetin from Cotinus coggygria leaves with polyamide

Authors

XU Na, School of Chemistry and Biology Engineering, Changsha University of Science＆ Technology, Changsha, Hunan 410114, China
XU Kaiyang, School of Chemistry and Biology Engineering, Changsha University of Science＆ Technology, Changsha, Hunan 410114, China
FANG Fang, School of Chemistry and Biology Engineering, Changsha University of Science＆ Technology, Changsha, Hunan 410114, China
SHU Xiaoshun, School of Chemistry and Biology Engineering, Changsha University of Science＆ Technology, Changsha, Hunan 410114, China
LI Chiling, School of Chemistry and Biology Engineering, Changsha University of Science＆ Technology, Changsha, Hunan 410114, China

Abstract

To study the separation and purification of fisetin from Cotinus coggygria leaves with polyamide by using the adsorption rate and elution rate of fisetin as index. The effects of sample flow rate, sample concentration, concentration of eluent and dosage on purification process were investigated from static and dynamic adsorption. The optimum process conditions were as followed: the sample flow rate was 1 mL/min, the sample concentration was 0.535 8 mg/mL, the sample volume was 2 BV, the eluent was 70% ethanol, and the eluent dosage was 4 BV. Conclusion: The crude extract of Cotinus coggygria leaves was refined three times under optimum conditions, which increased from 10.30% to 79.82%, and the purity increased 6.75 times.

Publication Date

12-28-2017

First Page

160

Last Page

163

DOI

10.13652/j.issn.1003-5788.2017.12.032

Recommended Citation

Na, XU; Kaiyang, XU; Fang, FANG; Xiaoshun, SHU; and Chiling, LI (2017) "Study on separation and purification process of fisetin from Cotinus coggygria leaves with polyamide," Food and Machinery: Vol. 33: Iss. 12, Article 32.
DOI: 10.13652/j.issn.1003-5788.2017.12.032
Available at: https://www.ifoodmm.cn/journal/vol33/iss12/32

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