Structural characterization and antioxiant, moisture-absording and moisture-retention properties of polysaccharides of Sophora japonica L.

Authors

LI Caixia, College of Agriculture and Biotechnology, Hexi University, Zhangye, Gansu 734000, China; Key Laboratory of Hexi Corridor Resources Utilization of Gansu Universities, Zhangye, Gansu 734000, China
ZHENG Xue, College of Agriculture and Biotechnology, Hexi University, Zhangye, Gansu 734000, China
GAO Haining, College of Agriculture and Biotechnology, Hexi University, Zhangye, Gansu 734000, China; Key Laboratory of Hexi Corridor Resources Utilization of Gansu Universities, Zhangye, Gansu 734000, China
ZHANG Yong, College of Agriculture and Biotechnology, Hexi University, Zhangye, Gansu 734000, China; Key Laboratory of Hexi Corridor Resources Utilization of Gansu Universities, Zhangye, Gansu 734000, China

Abstract

The polysaccharides from the seeds and peel of Sophora japonica L. were extracted respectively by double enzymes, The basic structure of polysaccharides were identified by using infrared spectroscopy,and antioxidant activity, moisture absorption and retention capacities of polysaccharides were evaluated. The results indicated that the polysaccharides extraction yield of Sophora japonica L. seeds and peel were 11.6% and 24.7% respectively. The IR spectrum indicated peel and seed polysaccharides had typical characteristic absorption peaks of polysaccharides and pyran ring structure, seed polysaccharides contained a-glycosidic bonds, peel polysaccharides contained α and β glycosidic bonds.The radical scavenging experiment showed that two different polysaccharides of Sophora japonica L. had a certain radical scavenging capacity. Within a certain concentration range, the radical scavenging activity of seeds polysaccharides was better than that of peel polysaccharides, and had the positive correlations with concentration of polysaccharides. Compared with the positive control vitamin C(V_C), the ABTS and DPPH radical scavenging ability of two polysaccharides were weak, but the scavenging effect of seeds polysaccharide on superoxide anion was better than that of V_C. Compared with the positive control EDTA, determination of two polysaccharides on ferrous ion chelating ability were weak. In the same conditions, moisture absorption capacities of two polysaccharides of sophora fructus were close to that of chitosan but lower than that of glycerin. Moisture retention capacities of two polysaccharides of sophora fructus were equivalent, lower than glycerin, but higher than chitosan. These results demonstrated that polysaccharides of Sophora japonica L. had good radical scavenging activity and moisture retention capacity which had the development and utilization value.

Publication Date

12-28-2017

First Page

17

Last Page

22

DOI

10.13652/j.issn.1003-5788.2017.12.004

Recommended Citation

Caixia, LI; Xue, ZHENG; Haining, GAO; and Yong, ZHANG (2017) "Structural characterization and antioxiant, moisture-absording and moisture-retention properties of polysaccharides of Sophora japonica L.," Food and Machinery: Vol. 33: Iss. 12, Article 4.
DOI: 10.13652/j.issn.1003-5788.2017.12.004
Available at: https://www.ifoodmm.cn/journal/vol33/iss12/4

References

[1] 国家药典委员会. 中华人民共和国药典: 第一部[M]. 北京: 中国医药科技出版社, 2015: 355.
[2] 陈明珠, 申艳艳, 赵越, 等. 槐角黄酮的体外抗氧化活性研究[J]. 食品工业科技, 2013, 34(4): 94-96.
[3] 刘景东, 刘惠敏, 王憬, 等. 槐角多糖含量测定方法的研究[J]. 白求恩军医学院学报, 2007, 5(3): 172-174.
[4] ERTAN A, SELIM K, ONDER P. Synergistic effect of the locust bean gum on the thermal phase transitions of k-carrageenan gels[J]. Food Hydrocolloids, 2009, 23(2): 451-459.
[5] 卞云霞, 付蕊, 刘英超. 槐豆多糖及其配合物的抑菌性研究[J]. 大连民族学院学报, 2009, 11(9): 70-71.
[6] 陈刚, 郭晓蕾, 宝丽. 银耳, 麦冬, 燕麦多糖的抗氧化活性及吸湿保湿性能研究[J]. 中华中医药学刊, 2013, 31(1): 212-214.
[7] 丁保金, 金丽琴, 吕建新. 多糖的生物活性研究进展[J]. 中国药学杂志, 2004, 39(8): 561-564.
[8] 赵琪, 赵利, 杨玉娈, 等. 河蚬多糖分离纯化及抗氧化、抗肿瘤活性研究[J]. 食品与机械, 2017, 33(4): 127-132, 138.
[9] 张斌, 张璐, 李沙沙, 等. 植物多糖与化妆品的联系[J]. 辽宁中医药大学学报, 2013, 15(1): 109-111.
[10] 宋慧, 苗敬芝, 董玉玮. 双酶法提取大豆多糖及其抗氧化性研究[J]. 中国食品添加剂, 2012(5): 89-93.
[11] 李彩霞, 李复兴, 李鹏, 等. 国槐叶黄酮的抗氧化活性研究[J]. 天然产物研究与开发, 2013, 25(5): 676-680.
[12] GHAVI P P. The extraction process optimization of antioxidant polysaccharides from Marshmallow (Althaea officinalis L.) roots[J]. International Journal of Biological Macromolecules, 2015, 75: 51-57.
[13] 李合生. 现代植物生理学[M]. 北京: 高等教育出版社, 2002: 32-34.
[14] 王凌, 孙利芹, 赵小惠. 一种微藻多糖的理化性质及抗氧化保湿活性[J]. 精细化工, 2012, 29(1): 20-25.
[15] 张华锋, 张杰, 刘炯, 等. 首乌藤多糖的分离纯化及初步结构解析[J]. 中国实验方剂学杂志, 2013, 19(18): 5-8.
[16] 于明, 朱文学, 郭菡, 等. 响应面分析法优化超声提取槐豆胶工艺[J]. 食品科学, 2013, 34(2): 114-118.
[17] 刘春兰, 徐斯凡. 少数民族地区药用植物多糖的化学与药理[M]. 北京: 中央民族大学出版社, 2008: 39-41.
[18] 张惟杰. 糖复合物生化研究技术[M]. 杭州: 浙江大学出版社, 1998: 196-198.
[19] 魏银花, 申迎宾, 王立, 等. 紫米多酚提取工艺及抗氧化活性研究[J]. 食品与机械, 2013, 29(3): 111-115.
[20] 范现丽, 王高, 王宏, 等. 香桃木叶片粗多酚的抗氧化活性的研究[J]. 植物研究, 2009, 29(3): 375-379.
[21] MANIAN R, ANUSUYA N, SIDDHURAJU P, et al. The antioxidant activity and free radical scavenging potential two different solvent extracts of Camellia sinensis(L)O.kuntz, Ficus bengalensis L. and Ficus racemosa L[J]. Food Chemistry, 2008, 107(3): 1 000-1 007.
[22] 方艳夕, 周丽丽, 叶强, 等. 槐角多糖及其水解物中单糖的检测[J]. 安徽科技学院学报, 2017, 31(1): 50-53.
[23] 肖锡湘, 上官新晨. 多糖的应用研究[J]. 中国食物与营养, 2006, 12(5): 21-22.
[24] 王阳, 霍秀文, 周翼虎, 等. 不同品种山药块茎多糖和黏液质多糖对自由基清除能力的研究[J]. 辽宁大学学报, 2012, 39(4): 354-360.
[25] ZHANG Ling-wen, JI Hong-fang, DU Ai-lin, et al. Characterization and antioxidant properties of polysaccharides from flowers of Sophora japonica L. (Huaihua) [J]. Journal of Medicinal Plants Research, 2013, 7(21): 1 543-1 549.