Abstract
The aim of this work was to establish a highly sensitive quantitative colloidal gold immunochromatographic assay with a test strip reader for the detection of aflatoxin B1 using colloidal gold as label. When the labeling pH was 6.0, the concentration of antigen on T-line was 1.5 mg/mL, the concentration of antibody labeled on the colloidal gold was 4 μg/mL, and the dosage of gold-labeled antibody was 1.2 μL, the sensitivity of the assay was 5.7 ng/L. The results of accelerated aging experiment indicated that the strips shelf time was more than a year. It was proved that this method can meet the needs of the rapid detection of food when the strip was applied to detect cereals and soybean sauce.
Publication Date
2-28-2017
First Page
51
Last Page
55
DOI
10.13652/j.issn.1003-5788.2017.02.011
Recommended Citation
Jing, LIU; Shuying, WANG; Daofeng, LIU; and Weihua, LAI
(2017)
"Development of Quantitative Sensitive Immunochromatographic Assays for Detection of Aflatoxin B1,"
Food and Machinery: Vol. 33:
Iss.
2, Article 11.
DOI: 10.13652/j.issn.1003-5788.2017.02.011
Available at:
https://www.ifoodmm.cn/journal/vol33/iss2/11
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