Study on acivity and structure analysis of ectracts from Chinese chestnut shell by ethyl acetate

Authors

SHI Lingling, College of Biological Science and Biotechnology, Beijing Forestry University, Beijing 100083, China
WANG Jianzhong, College of Biological Science and Biotechnology, Beijing Forestry University, Beijing 100083, China
LIU Yujun, College of Biological Science and Biotechnology, Beijing Forestry University, Beijing 100083, China
MA Chao, College of Biological Science and Biotechnology, Beijing Forestry University, Beijing 100083, China
LI Yijing, College of Biological Science and Biotechnology, Beijing Forestry University, Beijing 100083, China
DAI Jianrong, College of Biological Science and Biotechnology, Beijing Forestry University, Beijing 100083, China
CAO Yang, College of Biological Science and Biotechnology, Beijing Forestry University, Beijing 100083, China
TENG Wanzhao, College of Biological Science and Biotechnology, Beijing Forestry University, Beijing 100083, China

Abstract

Investigated and analyzed the activity of ethyl acetate extract of chestnut shell and the components by UPLC—MS/MS. Antioxidant activities were carried out by DPPH radical scavenging assays and ABTS radical scavenging assays. The inhibitory activity of alpha glycosidase enzymes was measured by PNPG method. Qualitative analysis was performed by UPLC—MS/MS for analysis of major active components in ethyl acetate extracts. It determined that ethyl acetate extracts of Chestnut shell possessed remarkable DPPH radical scavenging activity, ABTS radical scavenging activity and high inhibitory activity of alpha glycosidase enzymes owing to the fat-soluble flavonoids are the main active components.

Publication Date

2-28-2017

First Page

130

Last Page

134

DOI

10.13652/j.issn.1003-5788.2017.02.028

Recommended Citation

Lingling, SHI; Jianzhong, WANG; Yujun, LIU; Chao, MA; Yijing, LI; Jianrong, DAI; Yang, CAO; and Wanzhao, TENG (2017) "Study on acivity and structure analysis of ectracts from Chinese chestnut shell by ethyl acetate," Food and Machinery: Vol. 33: Iss. 2, Article 28.
DOI: 10.13652/j.issn.1003-5788.2017.02.028
Available at: https://www.ifoodmm.cn/journal/vol33/iss2/28

References

［1］ 周丹, 洪珊茹, 赵巧娇, 等. 板栗栗蓉的制作研究［J］. 食品科技, 2014, 39(3): 61-64.
［2］ YAO Zeng-yu, QI Jian-hua, WANG Li-hua. Equilibrium, kinetic and thermodynamic studies on the biosorption of Cu(II) onto chestnut shell［J］. Journal of Hazardous Materials, 2010, 174(1/3): 137-143.
［3］ DONG Ya-chen, DAI Yi-ning, XU Teng-yang, et al. Biodegradation of chestnut shell and lignin-modifying enzymes production by the white-rot fungi Dichomitus squalens, Phlebia radiata［J］. Bioprocess and Biosystems Engineering, 2014, 37(5): 755-764.
［4］ PIRRA A, LUCAS M S, PERES J A. Aerobic Biological Treatment of Chestnut Processing Wastewater［J］. Water, Air, & Soil Pollution, 2012, 223(7): 3 721-3 728.
［5］ 赵德义, 高文海, 花成文, 等. 板栗壳化学成分的初步研究［J］. 陕西林业科技, 2003(2): 1-3.
［6］ 郑佳欣, 李怡婧, 汪晨阳, 等. 板栗壳鞣质提取及其对DPPH自由基清除活性的研究［J］. 食品工业科技, 2016, 37(3): 211-215.
［7］ NOH J R, KIM Y H, GANG G T, et al. Hepatoprotective effects of chestnut (Castanea crenata) inner shell extract against chronic ethanol-induced oxidative stress in C57BL/6 mice［J］. Food & Chemical Toxicology An International Journal Published for the British Industrial Biological Research Association, 2011, 49(7): 1 537-1 543.
［8］ VZQUEZ G, FONTENLA E, SANTOS J, et al. Antioxidant activity and phenolic content of chestnut (Castanea sativa) shell and eucalyptus (Eucalyptus globulus) bark extracts［J］. Industrial Crops & Products, 2008, 28(3): 279-285.
［9］ 李莉, 顾欣, 崔洁, 等. 板栗壳棕色素抗氧化性研究与红外光谱分析［J］. 食品与发酵工业, 2011, 37(3): 41-45.
［10］ 王慧. 板栗壳色素提取工艺研究［J］. 食品研究与开发, 2014, 35(2): 47-50.
［11］ VZQUEZ G, FREIRE M S, SANTOS J, et al. Optimisation of polyphenols extraction from chestnut shell by response surface methodology［J］. Waste and Biomass Valorization, 2010, 1(2): 219-225.
［12］ YOU Ting-ting, ZHOU Su-kun, WEN Jia-long, et al. Chemical composition, properties, and antimicrobial activity of the water-soluble pigments from Castanea mollissima shells［J］. Journal of Agricultural & Food Chemistry, 2014, 62(8): 1 936-1 944.
［13］ 刘莉, 唐新玥, 张欣珂, 等. 板栗壳中多酚的提取纯化及其抑制α-葡萄糖苷酶活性的研究［J］. 食品工业科技, 2015, 36(6): 265-268.
［14］ LUO Jian-guang, LI Lu, KONG Ling-yi. Preparative separation of phenylpropenoid glycerides from the bulbs of Lilium lancifolium, by high-speed counter-current chromatography and evaluation of their antioxidant activities［J］. Food Chemistry, 2012, 131(3): 1 056-1 062.
［15］ CHAPDELAINE P, TREMBLAY R R, DUB J Y. P-Nitrophenol-alpha-D-glucopyranoside as substrate for measurement of maltase activity in human semen［J］. Clinical Chemistry, 1978, 24(2): 208-211.
［16］ 焦中高, 刘杰超, 周红平, 等. 枣果中酚类物质的高效液相色谱分析［J］. 食品与发酵工业, 2008, 34(3): 133-136.
［17］ 钱瑾, 黎明, 吴嘉瑞, 等. 消渴丸中药成分对α-葡萄糖苷酶抑制作用的研究［J］. 中国实验方剂学杂志, 2012, 18(7): 173-176.
［18］ 单鸣秋, 钱雯, 高静, 等. UPLC—MS分析侧柏叶中黄酮类化合物［J］. 中国中药杂志, 2011, 36(12): 1 626-1 629.
［19］ 李亭亭, 徐新房, 王子健, 等. 牵牛子生品、炒品酚酸类成分的HPLC—MS分析［J］. 中医药学报, 2016, 44(1): 11-14.
［20］ 梁艳, 于世锋, 陈卫军, 等. 山奈酚的电喷雾质谱裂解途径［J］. 应用化学, 2009, 26(10): 1 250-1 252.
［21］ 朱慧明. 基于液质联用技术的芪苈强心胶囊中多组分分析与药代动力学研究［D］. 石家庄: 河北医科大学, 2014: 126-128.
［22］ 师仁丽, 翟龙飞, 于文龙, 等. 利用DAD—HPLC和LC—MS法检测金丝小枣中黄酮类化合物［J］. 食品科学, 2016, 37(16): 123-127.
［23］ 李宇航, 戴海学, 汪明明, 等. 槲皮素的电喷雾离子阱质谱分析［J］. 质谱学报, 2009, 30(6): 374-378.
［24］ 朱瑞芝, 王凯, 吴新华, 等. 超高效液相色谱—质谱法同时快速测定烟叶中的几种芳香酸和酚酸［J］. 分析试验室, 2009, 28(10): 108-112.
［25］ 罗益远, 刘娟秀, 刘廷, 等. UPLC—MS/MS法同时测定何首乌中二苯乙烯、蒽醌、黄酮及酚酸类成分［J］. 质谱学报, 2016, 37(4): 327-335.
［26］ 方玲玲, 肖作兵, 牛云蔚, 等. 超高效液相色谱串联质谱法测定樱桃酒中的5种酚酸［J］. 食品与发酵工业, 2011, 37(6): 172-176.