The antioxidant activities and protection on DNA oxidative damage of flavonoid from the flowers of Abelmoschus manihot

Authors

LI Wang, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi, Jiangsu 214122, China;The Key Laboratory of Food Colloids and Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, China
SUN Peidong, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi, Jiangsu 214122, China;The Key Laboratory of Food Colloids and Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, China
JI Xiaotong, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi, Jiangsu 214122, China;The Key Laboratory of Food Colloids and Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, China
LU Shuai, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi, Jiangsu 214122, China;The Key Laboratory of Food Colloids and Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, China

Abstract

The ethanol extracts of flowers from Abelmoschus manihot was extracted by petroleum ether、ethyl acetate and n-butyl alcohol, and four different polar portions were obtained. The scavenging effect of different phases on DPPH·, ·OH and H₂O₂ were studied to research the antioxidant activities of each phase. Moreover, the protection of n-butyl alcohol phase on DNA oxidative damage was also studied, and the flavonoids component was identified by HPLC-MS. The results showed that the ethyl acetate phase had the best scavenging effect on DPPH·, with IC₅₀ value 0.038 6 mg/mL. However, the n-butyl alcohol phase had scavenge ·OH and H₂O₂ best, and the IC₅₀ values were 0.225 0 and 0.575 7 mg/mL, respectively. The n-butyl alcohol phase was proved to show protective effect in some degree on DNA oxidative damage., and totally 11 kinds of flavonoids were found in the n-butyl alcohol phase.

Publication Date

2-28-2017

First Page

135

Last Page

139

DOI

10.13652/j.issn.1003-5788.2017.02.029

Recommended Citation

Wang, LI; Peidong, SUN; Xiaotong, JI; and Shuai, LU (2017) "The antioxidant activities and protection on DNA oxidative damage of flavonoid from the flowers of Abelmoschus manihot," Food and Machinery: Vol. 33: Iss. 2, Article 29.
DOI: 10.13652/j.issn.1003-5788.2017.02.029
Available at: https://www.ifoodmm.cn/journal/vol33/iss2/29

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